Tolypothrix campylonemoides VB511288
Average proteome isoelectric point is 6.54
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6864 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0C2PPN7|A0A0C2PPN7_9CYAN Short-chain dehydrogenase OS=Tolypothrix campylonemoides VB511288 OX=1245935 GN=SD81_28825 PE=3 SV=1
MM1 pKa = 7.39 QIQFRR6 pKa = 11.84 EE7 pKa = 4.21 INPFDD12 pKa = 3.26 LWIWLEE18 pKa = 3.86 FSTIPSEE25 pKa = 4.14 QEE27 pKa = 3.28 KK28 pKa = 10.76 KK29 pKa = 10.33 YY30 pKa = 10.85 VEE32 pKa = 4.18 EE33 pKa = 4.59 VFNSWFYY40 pKa = 11.05 LGKK43 pKa = 10.59 LGAFNAEE50 pKa = 3.81 NLQVQEE56 pKa = 4.22 TGLDD60 pKa = 3.91 LSYY63 pKa = 10.52 MDD65 pKa = 5.37 YY66 pKa = 11.24 DD67 pKa = 3.56 PEE69 pKa = 6.75 GYY71 pKa = 10.18 DD72 pKa = 3.44 KK73 pKa = 11.36 SLLALMHH80 pKa = 6.49 NMGQFEE86 pKa = 4.9 YY87 pKa = 9.84 EE88 pKa = 4.39 GTWARR93 pKa = 11.84 CWFDD97 pKa = 4.77 LGTSDD102 pKa = 6.2 AIALDD107 pKa = 3.43 ILINALKK114 pKa = 10.18 QLNQEE119 pKa = 4.25 YY120 pKa = 8.11 VTIEE124 pKa = 3.51 QLYY127 pKa = 10.33 IGGEE131 pKa = 3.99 NEE133 pKa = 3.95 DD134 pKa = 3.51 WSVEE138 pKa = 3.82 DD139 pKa = 4.09 SEE141 pKa = 4.69 NGRR144 pKa = 11.84 SSFIYY149 pKa = 10.68 DD150 pKa = 3.23 NN151 pKa = 3.84
Molecular weight: 17.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.75
IPC2_protein 3.91
IPC_protein 3.859
Toseland 3.668
ProMoST 3.986
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.706
Rodwell 3.694
Grimsley 3.579
Solomon 3.808
Lehninger 3.757
Nozaki 3.935
DTASelect 4.088
Thurlkill 3.706
EMBOSS 3.719
Sillero 3.961
Patrickios 0.985
IPC_peptide 3.808
IPC2_peptide 3.948
IPC2.peptide.svr19 3.865
Protein with the highest isoelectric point:
>tr|A0A0C2L8C7|A0A0C2L8C7_9CYAN Enoyl-CoA hydratase OS=Tolypothrix campylonemoides VB511288 OX=1245935 GN=SD81_43775 PE=4 SV=1
MM1 pKa = 7.0 ATTWQMHH8 pKa = 5.66 RR9 pKa = 11.84 ARR11 pKa = 11.84 PVKK14 pKa = 10.16 VHH16 pKa = 6.56 GGAASPAARR25 pKa = 11.84 EE26 pKa = 3.81 GHH28 pKa = 5.15 RR29 pKa = 11.84 RR30 pKa = 11.84 GRR32 pKa = 11.84 GIHH35 pKa = 6.41 AFAGMTAGTRR45 pKa = 11.84 QRR47 pKa = 11.84 GRR49 pKa = 11.84 ARR51 pKa = 11.84 SRR53 pKa = 11.84 PRR55 pKa = 11.84 RR56 pKa = 11.84 TGAAAARR63 pKa = 11.84 SRR65 pKa = 11.84 RR66 pKa = 11.84 IGRR69 pKa = 11.84 KK70 pKa = 8.1 RR71 pKa = 11.84 LAWLRR76 pKa = 11.84 IPHH79 pKa = 6.44 HH80 pKa = 6.71 LGPP83 pKa = 4.72
Molecular weight: 9.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.509
IPC2_protein 11.155
IPC_protein 12.764
Toseland 12.925
ProMoST 13.437
Dawson 12.925
Bjellqvist 12.925
Wikipedia 13.408
Rodwell 12.471
Grimsley 12.969
Solomon 13.437
Lehninger 13.334
Nozaki 12.925
DTASelect 12.925
Thurlkill 12.925
EMBOSS 13.437
Sillero 12.925
Patrickios 12.193
IPC_peptide 13.437
IPC2_peptide 12.427
IPC2.peptide.svr19 9.187
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6863
1
6864
2116098
30
3801
308.3
34.32
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.782 ± 0.049
0.981 ± 0.01
4.876 ± 0.023
6.348 ± 0.031
3.796 ± 0.021
6.892 ± 0.035
2.078 ± 0.021
6.071 ± 0.031
4.657 ± 0.03
10.753 ± 0.036
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.784 ± 0.014
3.94 ± 0.026
4.757 ± 0.023
5.367 ± 0.03
6.03 ± 0.054
6.09 ± 0.03
5.396 ± 0.028
7.154 ± 0.025
1.37 ± 0.013
2.876 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here