Algibacter alginicilyticus
Average proteome isoelectric point is 6.62
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3236 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0P0DD31|A0A0P0DD31_9FLAO Altronate hydrolase OS=Algibacter alginicilyticus OX=1736674 GN=APS56_12635 PE=3 SV=1
MM1 pKa = 7.18 ITKK4 pKa = 10.01 QSLRR8 pKa = 11.84 IFLSTVFFSFFIFLSCDD25 pKa = 3.39 DD26 pKa = 4.72 DD27 pKa = 5.85 NEE29 pKa = 4.77 INDD32 pKa = 3.63 ATNPNFSNLITSISSAPGLEE52 pKa = 4.73 FIFEE56 pKa = 4.29 GTISDD61 pKa = 4.15 DD62 pKa = 4.12 NGIEE66 pKa = 4.23 NININYY72 pKa = 9.23 DD73 pKa = 2.64 NWYY76 pKa = 8.56 VDD78 pKa = 3.4 KK79 pKa = 11.17 NITFDD84 pKa = 4.03 EE85 pKa = 4.31 PLKK88 pKa = 10.65 EE89 pKa = 3.91 YY90 pKa = 10.72 NLNYY94 pKa = 10.04 KK95 pKa = 10.18 FLVPEE100 pKa = 4.13 EE101 pKa = 4.44 EE102 pKa = 4.74 EE103 pKa = 4.49 PNSSHH108 pKa = 6.47 TIKK111 pKa = 10.62 ISATDD116 pKa = 3.17 IAGNITTYY124 pKa = 11.21 DD125 pKa = 3.63 VIVSLDD131 pKa = 3.61 YY132 pKa = 10.04 DD133 pKa = 3.75 TTLPQVAFISPISGSSNTVGDD154 pKa = 4.04 LVEE157 pKa = 4.56 LNIAFSDD164 pKa = 3.75 NKK166 pKa = 10.19 VLDD169 pKa = 4.14 SIIVKK174 pKa = 9.14 TEE176 pKa = 3.7 SLDD179 pKa = 3.81 YY180 pKa = 10.39 EE181 pKa = 5.03 VKK183 pKa = 10.85 LKK185 pKa = 10.69 MPDD188 pKa = 2.81 NTLNYY193 pKa = 10.18 NFTDD197 pKa = 3.46 SVEE200 pKa = 4.1 IPLTGISGAIEE211 pKa = 3.96 FTATGIDD218 pKa = 3.33 KK219 pKa = 10.14 TGNTTTVYY227 pKa = 9.54 STILVGEE234 pKa = 4.09 KK235 pKa = 10.75 DD236 pKa = 4.1 EE237 pKa = 4.43 IFNMYY242 pKa = 10.62 AVGTSTWYY250 pKa = 10.07 EE251 pKa = 3.82 WDD253 pKa = 3.52 PSKK256 pKa = 10.75 ATEE259 pKa = 3.77 MWKK262 pKa = 10.49 NPDD265 pKa = 3.37 NNDD268 pKa = 2.78 WFVLEE273 pKa = 5.26 FYY275 pKa = 10.08 YY276 pKa = 11.09 TMGNGVKK283 pKa = 10.49 FIGQLDD289 pKa = 3.85 WEE291 pKa = 4.63 PNNWGTDD298 pKa = 3.59 PNDD301 pKa = 3.06 SSKK304 pKa = 10.7 IINSQDD310 pKa = 2.79 SGTIEE315 pKa = 4.19 FPEE318 pKa = 3.95 EE319 pKa = 4.1 GYY321 pKa = 10.8 YY322 pKa = 10.3 HH323 pKa = 7.47 VEE325 pKa = 3.93 FNPYY329 pKa = 7.75 TLEE332 pKa = 3.81 YY333 pKa = 8.93 TYY335 pKa = 11.31 EE336 pKa = 4.15 KK337 pKa = 10.43 MEE339 pKa = 3.83 VDD341 pKa = 3.32 VDD343 pKa = 4.07 VKK345 pKa = 11.35 EE346 pKa = 4.14 NMYY349 pKa = 11.46 LMGNGFAGYY358 pKa = 10.53 DD359 pKa = 4.0 LDD361 pKa = 4.49 WNPADD366 pKa = 5.25 AIPMEE371 pKa = 5.0 KK372 pKa = 10.26 DD373 pKa = 3.41 SNGNPYY379 pKa = 10.29 VFTINVEE386 pKa = 3.96 ITEE389 pKa = 4.14 DD390 pKa = 3.36 TSLKK394 pKa = 10.79 FIGQTDD400 pKa = 3.21 GWSPFDD406 pKa = 4.04 CGFEE410 pKa = 4.26 VGGEE414 pKa = 4.16 TILPVNYY421 pKa = 9.45 IKK423 pKa = 10.94 CKK425 pKa = 10.18 TGDD428 pKa = 3.26 GSQDD432 pKa = 3.25 LKK434 pKa = 11.21 FKK436 pKa = 10.48 NQAGTYY442 pKa = 8.5 TITFDD447 pKa = 3.76 YY448 pKa = 10.62 FLLRR452 pKa = 11.84 ATIHH456 pKa = 5.91 QYY458 pKa = 10.0 NN459 pKa = 3.49
Molecular weight: 51.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.718
IPC2_protein 3.872
IPC_protein 3.884
Toseland 3.668
ProMoST 4.024
Dawson 3.859
Bjellqvist 4.012
Wikipedia 3.77
Rodwell 3.706
Grimsley 3.579
Solomon 3.859
Lehninger 3.808
Nozaki 3.961
DTASelect 4.19
Thurlkill 3.706
EMBOSS 3.783
Sillero 3.999
Patrickios 1.151
IPC_peptide 3.859
IPC2_peptide 3.986
IPC2.peptide.svr19 3.884
Protein with the highest isoelectric point:
>tr|A0A0P0CQ50|A0A0P0CQ50_9FLAO Uncharacterized protein OS=Algibacter alginicilyticus OX=1736674 GN=APS56_07705 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.01 RR4 pKa = 11.84 TFQPSKK10 pKa = 9.13 RR11 pKa = 11.84 KK12 pKa = 9.48 RR13 pKa = 11.84 RR14 pKa = 11.84 NKK16 pKa = 9.49 HH17 pKa = 3.94 GFRR20 pKa = 11.84 EE21 pKa = 4.27 RR22 pKa = 11.84 MASANGRR29 pKa = 11.84 KK30 pKa = 9.04 VLARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 10.09 GRR40 pKa = 11.84 KK41 pKa = 7.97 KK42 pKa = 10.62 LSVSSEE48 pKa = 4.03 TRR50 pKa = 11.84 HH51 pKa = 5.96 KK52 pKa = 10.68 KK53 pKa = 9.8
Molecular weight: 6.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.352
IPC2_protein 10.76
IPC_protein 12.325
Toseland 12.501
ProMoST 12.983
Dawson 12.501
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.296
Grimsley 12.53
Solomon 12.983
Lehninger 12.881
Nozaki 12.486
DTASelect 12.486
Thurlkill 12.486
EMBOSS 12.983
Sillero 12.486
Patrickios 12.018
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.066
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3236
0
3236
1177855
50
3879
364.0
41.13
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.972 ± 0.04
0.735 ± 0.014
5.593 ± 0.03
6.346 ± 0.035
5.223 ± 0.033
6.243 ± 0.045
1.81 ± 0.021
8.363 ± 0.045
7.953 ± 0.055
9.152 ± 0.043
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.065 ± 0.022
6.886 ± 0.043
3.328 ± 0.022
3.243 ± 0.024
3.137 ± 0.025
6.641 ± 0.035
5.955 ± 0.043
6.025 ± 0.032
1.08 ± 0.016
4.249 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here