BK polyomavirus (BKPyV) (Human polyomavirus 1)

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cossaviricota; Papovaviricetes; Sepolyvirales; Polyomaviridae; Betapolyomavirus

Average proteome isoelectric point is 7.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q0PCM6|Q0PCM6_POVBK Minor capsid protein OS=BK polyomavirus OX=1891762 PE=3 SV=1
MM1 pKa = 7.71APTKK5 pKa = 10.54RR6 pKa = 11.84KK7 pKa = 10.25GEE9 pKa = 4.26CPGAAPKK16 pKa = 10.27KK17 pKa = 9.07PKK19 pKa = 10.55EE20 pKa = 3.96PVQVPKK26 pKa = 10.86LLIKK30 pKa = 10.81GGVEE34 pKa = 3.79VLEE37 pKa = 4.53VKK39 pKa = 9.99TGVDD43 pKa = 3.63AITEE47 pKa = 4.29VEE49 pKa = 4.37CFLNPEE55 pKa = 4.38MGDD58 pKa = 3.29PDD60 pKa = 3.91NDD62 pKa = 3.24LRR64 pKa = 11.84GYY66 pKa = 8.45SLRR69 pKa = 11.84LTAEE73 pKa = 3.95TAFDD77 pKa = 3.71SDD79 pKa = 3.96SPDD82 pKa = 3.09RR83 pKa = 11.84KK84 pKa = 9.47MLPCYY89 pKa = 9.18STARR93 pKa = 11.84IPLPNLNEE101 pKa = 4.72DD102 pKa = 3.98LTCGNLLMWEE112 pKa = 4.08AVTVKK117 pKa = 10.14TEE119 pKa = 4.19VIGITSMLNLHH130 pKa = 6.71AGSQKK135 pKa = 8.81VHH137 pKa = 6.45EE138 pKa = 4.91NGGGKK143 pKa = 9.33PIQGSNFHH151 pKa = 6.63FFAVGGDD158 pKa = 3.83PLEE161 pKa = 4.33MQGVLMNYY169 pKa = 6.15RR170 pKa = 11.84TKK172 pKa = 10.72YY173 pKa = 10.14PEE175 pKa = 4.09GTVTPKK181 pKa = 10.87NPTAQSQVMNTDD193 pKa = 2.82HH194 pKa = 7.79KK195 pKa = 11.53AYY197 pKa = 10.42LDD199 pKa = 3.64KK200 pKa = 11.64NNAYY204 pKa = 9.1PVEE207 pKa = 4.23CWIPDD212 pKa = 3.47PSRR215 pKa = 11.84NEE217 pKa = 3.45NTRR220 pKa = 11.84YY221 pKa = 9.81FGTYY225 pKa = 8.7TGGEE229 pKa = 4.01NVPPVLHH236 pKa = 5.52VTNTATTVLLDD247 pKa = 3.84EE248 pKa = 4.66QGVGPLCKK256 pKa = 9.76ADD258 pKa = 3.67SLYY261 pKa = 11.29VSAADD266 pKa = 3.09ICGLFTNSSGTQQWRR281 pKa = 11.84GLPRR285 pKa = 11.84YY286 pKa = 9.33FKK288 pKa = 10.22IRR290 pKa = 11.84LRR292 pKa = 11.84KK293 pKa = 9.4RR294 pKa = 11.84SVKK297 pKa = 10.18NPYY300 pKa = 9.28PISFLLSDD308 pKa = 4.93LINRR312 pKa = 11.84RR313 pKa = 11.84TQRR316 pKa = 11.84VDD318 pKa = 3.18GQPMYY323 pKa = 11.3GMEE326 pKa = 4.2SQVEE330 pKa = 4.21EE331 pKa = 4.01VRR333 pKa = 11.84VFDD336 pKa = 4.45GTEE339 pKa = 3.84QLPGDD344 pKa = 4.33PDD346 pKa = 3.84MIRR349 pKa = 11.84YY350 pKa = 8.96IDD352 pKa = 3.87RR353 pKa = 11.84QGQLQTKK360 pKa = 7.5MVV362 pKa = 3.43

Molecular weight: 40.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q3C215|Q3C215_POVBK Small t antigen OS=BK polyomavirus OX=1891762 PE=4 SV=1
MM1 pKa = 7.53VLRR4 pKa = 11.84QLSRR8 pKa = 11.84QASVKK13 pKa = 10.14VSKK16 pKa = 8.91TWTGTKK22 pKa = 9.78KK23 pKa = 9.72RR24 pKa = 11.84AQRR27 pKa = 11.84ILIFILEE34 pKa = 4.41LLLEE38 pKa = 4.51FCRR41 pKa = 11.84GEE43 pKa = 4.32DD44 pKa = 4.5SVDD47 pKa = 3.39GKK49 pKa = 10.79NKK51 pKa = 9.27STTALPAVKK60 pKa = 10.32DD61 pKa = 3.9SVKK64 pKa = 10.92DD65 pKa = 3.45SS66 pKa = 3.5

Molecular weight: 7.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1183

66

362

236.6

26.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.27 ± 2.022

1.606 ± 1.086

5.325 ± 0.624

6.255 ± 0.291

3.635 ± 0.294

6.593 ± 0.805

1.522 ± 0.191

4.818 ± 0.752

5.241 ± 1.292

8.791 ± 0.972

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.536 ± 0.596

4.48 ± 0.709

5.748 ± 0.914

4.818 ± 0.356

6.678 ± 0.601

6.34 ± 0.959

6.932 ± 0.597

6.34 ± 0.618

1.691 ± 0.53

3.381 ± 0.401
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski