Fimbriimonas ginsengisoli Gsoil 348
Average proteome isoelectric point is 6.57
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4817 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A068NQ48|A0A068NQ48_9BACT Uncharacterized protein OS=Fimbriimonas ginsengisoli Gsoil 348 OX=661478 GN=OP10G_2310 PE=4 SV=1
MM1 pKa = 7.64 FSTLIDD7 pKa = 3.78 GAEE10 pKa = 4.24 FACGLQLQTGLDD22 pKa = 3.79 EE23 pKa = 5.3 PDD25 pKa = 4.25 DD26 pKa = 5.2 GIVCAEE32 pKa = 4.08 VVSACEE38 pKa = 4.16 SMDD41 pKa = 3.84 FRR43 pKa = 11.84 LDD45 pKa = 3.39 VQGDD49 pKa = 3.86 IEE51 pKa = 4.67 SHH53 pKa = 6.4 CNRR56 pKa = 11.84 SPQPFF61 pKa = 3.33
Molecular weight: 6.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.775
IPC2_protein 3.706
IPC_protein 3.605
Toseland 3.414
ProMoST 3.808
Dawson 3.617
Bjellqvist 3.783
Wikipedia 3.592
Rodwell 3.452
Grimsley 3.338
Solomon 3.579
Lehninger 3.541
Nozaki 3.77
DTASelect 3.948
Thurlkill 3.503
EMBOSS 3.592
Sillero 3.745
Patrickios 0.604
IPC_peptide 3.579
IPC2_peptide 3.706
IPC2.peptide.svr19 3.708
Protein with the highest isoelectric point:
>tr|A0A068NP97|A0A068NP97_9BACT Alpha/beta fold superfamily hydrolase OS=Fimbriimonas ginsengisoli Gsoil 348 OX=661478 GN=OP10G_1903 PE=4 SV=1
MM1 pKa = 7.57 GSKK4 pKa = 10.21 KK5 pKa = 10.32 LPRR8 pKa = 11.84 INHH11 pKa = 5.35 GMRR14 pKa = 11.84 AGLVALLIALPLLALGASMASHH36 pKa = 7.64 PASGHH41 pKa = 4.37 GTAPGALAAMAFGAVSMFGRR61 pKa = 11.84 RR62 pKa = 11.84 RR63 pKa = 11.84 TT64 pKa = 3.53
Molecular weight: 6.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.399
IPC2_protein 10.847
IPC_protein 12.442
Toseland 12.603
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.281
Grimsley 12.647
Solomon 13.1
Lehninger 13.013
Nozaki 12.603
DTASelect 12.603
Thurlkill 12.603
EMBOSS 13.1
Sillero 12.603
Patrickios 12.047
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.093
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4817
0
4817
1529550
37
2429
317.5
34.79
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.357 ± 0.043
0.863 ± 0.01
5.592 ± 0.025
5.947 ± 0.04
3.83 ± 0.022
8.585 ± 0.031
2.062 ± 0.016
4.821 ± 0.022
3.9 ± 0.029
9.619 ± 0.039
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.339 ± 0.018
3.044 ± 0.023
5.522 ± 0.026
3.241 ± 0.019
6.817 ± 0.039
6.013 ± 0.033
5.65 ± 0.042
7.514 ± 0.033
1.566 ± 0.018
2.717 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here