Streptomyces chattanoogensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7423 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0N1JW88|A0A0N1JW88_9ACTN Ferredoxin reductase OS=Streptomyces chattanoogensis OX=66876 GN=ADL29_33045 PE=4 SV=1
MM1 pKa = 7.22TRR3 pKa = 11.84FDD5 pKa = 3.34GALIEE10 pKa = 4.56FCQEE14 pKa = 3.21QWSGDD19 pKa = 3.73GTNGLALTVRR29 pKa = 11.84GRR31 pKa = 11.84TAEE34 pKa = 3.79GRR36 pKa = 11.84LLAGADD42 pKa = 3.99AQCSLAGLEE51 pKa = 5.25DD52 pKa = 3.72IDD54 pKa = 5.97LLVEE58 pKa = 4.0NCGCPFVSEE67 pKa = 5.94DD68 pKa = 3.4GMQDD72 pKa = 4.17AEE74 pKa = 4.95DD75 pKa = 4.03LLATAFPGADD85 pKa = 2.93VRR87 pKa = 11.84VYY89 pKa = 9.4EE90 pKa = 4.3TDD92 pKa = 3.72EE93 pKa = 4.48EE94 pKa = 4.65PGPPPGATRR103 pKa = 11.84ASPLWEE109 pKa = 4.24YY110 pKa = 10.76EE111 pKa = 4.57VTWEE115 pKa = 4.15TCEE118 pKa = 4.12SCSEE122 pKa = 4.19VV123 pKa = 3.24

Molecular weight: 13.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0N0XYE6|A0A0N0XYE6_9ACTN Molybdopterin dehydrogenase OS=Streptomyces chattanoogensis OX=66876 GN=ADL29_13295 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILASRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.36GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7423

0

7423

2369380

29

7326

319.2

34.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.874 ± 0.043

0.842 ± 0.008

5.895 ± 0.023

5.601 ± 0.026

2.744 ± 0.014

9.495 ± 0.025

2.432 ± 0.014

3.265 ± 0.016

2.206 ± 0.023

10.456 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.763 ± 0.013

1.717 ± 0.013

6.259 ± 0.023

2.84 ± 0.019

8.14 ± 0.027

4.876 ± 0.02

5.985 ± 0.022

8.062 ± 0.027

1.513 ± 0.012

2.036 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski