Tomato bright yellow mottle virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 7.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R9SAJ4|R9SAJ4_9GEMI Capsid protein OS=Tomato bright yellow mottle virus OX=858512 PE=3 SV=1
MM1 pKa = 7.47KK2 pKa = 10.2FVFLNSPLYY11 pKa = 9.19QTTAQNCQEE20 pKa = 4.05EE21 pKa = 4.66EE22 pKa = 4.56GNQEE26 pKa = 3.84TTNFSLVRR34 pKa = 11.84DD35 pKa = 4.02AQSTCTFTAEE45 pKa = 4.94DD46 pKa = 3.56MDD48 pKa = 3.96SRR50 pKa = 11.84TGEE53 pKa = 4.16RR54 pKa = 11.84ITRR57 pKa = 11.84RR58 pKa = 11.84QAEE61 pKa = 4.19NGVYY65 pKa = 8.35TWEE68 pKa = 4.29VQNPLYY74 pKa = 10.66FKK76 pKa = 9.41ITNVEE81 pKa = 3.97ILLHH85 pKa = 6.56ANTTVYY91 pKa = 10.37HH92 pKa = 4.87IQIRR96 pKa = 11.84FNHH99 pKa = 5.66NLRR102 pKa = 11.84RR103 pKa = 11.84ALDD106 pKa = 3.39LHH108 pKa = 6.17KK109 pKa = 10.62AYY111 pKa = 11.06LNFQVWTTSLTASGTTYY128 pKa = 10.5FYY130 pKa = 11.52LFLFLVNMYY139 pKa = 9.41FLKK142 pKa = 10.81FGVISII148 pKa = 4.54

Molecular weight: 17.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R9SAJ4|R9SAJ4_9GEMI Capsid protein OS=Tomato bright yellow mottle virus OX=858512 PE=3 SV=1
MM1 pKa = 7.86PKK3 pKa = 9.94RR4 pKa = 11.84YY5 pKa = 9.11PPFRR9 pKa = 11.84TTAGISKK16 pKa = 10.14ISRR19 pKa = 11.84SSNMSPRR26 pKa = 11.84GGIRR30 pKa = 11.84PKK32 pKa = 10.2FDD34 pKa = 4.27KK35 pKa = 10.87AAEE38 pKa = 4.12WVNRR42 pKa = 11.84PMYY45 pKa = 9.79RR46 pKa = 11.84KK47 pKa = 8.95PRR49 pKa = 11.84IYY51 pKa = 9.54RR52 pKa = 11.84TLRR55 pKa = 11.84SPDD58 pKa = 3.03IPRR61 pKa = 11.84GCEE64 pKa = 4.19GPCKK68 pKa = 10.21VQSFEE73 pKa = 3.83QRR75 pKa = 11.84HH76 pKa = 5.53DD77 pKa = 3.55VSHH80 pKa = 6.05VGKK83 pKa = 9.89VLCISDD89 pKa = 3.56VTRR92 pKa = 11.84GNGITHH98 pKa = 6.78RR99 pKa = 11.84VGKK102 pKa = 9.44RR103 pKa = 11.84FCVKK107 pKa = 9.92SVYY110 pKa = 10.24ILGKK114 pKa = 10.11VWMDD118 pKa = 4.51DD119 pKa = 3.58NIKK122 pKa = 10.64LKK124 pKa = 10.77NHH126 pKa = 5.66TNSVLFWLVRR136 pKa = 11.84DD137 pKa = 3.69RR138 pKa = 11.84RR139 pKa = 11.84PYY141 pKa = 8.05GTPMDD146 pKa = 4.6FGQVFNMYY154 pKa = 10.66DD155 pKa = 3.86NEE157 pKa = 4.34PSTATVKK164 pKa = 10.82NDD166 pKa = 2.81LRR168 pKa = 11.84DD169 pKa = 4.23RR170 pKa = 11.84IQVMHH175 pKa = 7.06RR176 pKa = 11.84FSAKK180 pKa = 8.35VTGGQYY186 pKa = 11.02ASNEE190 pKa = 3.93QAVVRR195 pKa = 11.84RR196 pKa = 11.84FWKK199 pKa = 9.79VYY201 pKa = 8.04NHH203 pKa = 5.68VVYY206 pKa = 10.73NHH208 pKa = 5.8QEE210 pKa = 3.51AAKK213 pKa = 10.69YY214 pKa = 9.17EE215 pKa = 4.1IHH217 pKa = 6.56TEE219 pKa = 3.92NALLLYY225 pKa = 7.29MACTHH230 pKa = 7.07ASNPVYY236 pKa = 9.86ATLKK240 pKa = 9.47IRR242 pKa = 11.84IYY244 pKa = 10.6FYY246 pKa = 11.36DD247 pKa = 4.19SILNN251 pKa = 3.75

Molecular weight: 29.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

934

146

389

233.5

26.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.46 ± 0.541

1.713 ± 0.425

4.283 ± 0.818

4.283 ± 0.947

4.818 ± 0.671

5.139 ± 0.358

3.533 ± 0.297

6.103 ± 0.341

5.139 ± 0.824

7.173 ± 0.87

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.141 ± 0.35

6.21 ± 0.594

4.925 ± 0.75

4.069 ± 0.514

7.923 ± 1.364

7.709 ± 0.941

6.959 ± 1.521

5.675 ± 1.07

1.927 ± 0.182

4.711 ± 0.409
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski