Paraburkholderia sp. BL10I2N1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Paraburkholderia; unclassified Paraburkholderia

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7997 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4V3BQB3|A0A4V3BQB3_9BURK Phasin family protein OS=Paraburkholderia sp. BL10I2N1 OX=1938796 GN=B0G77_4912 PE=4 SV=1
MM1 pKa = 7.18EE2 pKa = 5.36TLTQPASATPGVMPGLLEE20 pKa = 3.99FTPQAAAKK28 pKa = 8.83VASLIEE34 pKa = 4.23EE35 pKa = 4.21EE36 pKa = 4.73GNPNLKK42 pKa = 10.22LRR44 pKa = 11.84LYY46 pKa = 10.67VSGGGCSGLQYY57 pKa = 11.24GFAFDD62 pKa = 5.18DD63 pKa = 3.81QVADD67 pKa = 5.01DD68 pKa = 5.31DD69 pKa = 4.42MQTVTDD75 pKa = 4.79GVTLLVDD82 pKa = 4.04SMSQQYY88 pKa = 10.77LPGARR93 pKa = 11.84VDD95 pKa = 3.98YY96 pKa = 11.02EE97 pKa = 4.51DD98 pKa = 4.21GLEE101 pKa = 3.97GSRR104 pKa = 11.84FVIHH108 pKa = 6.52NPNAQSTCGCGSSFSVV124 pKa = 3.54

Molecular weight: 13.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R6F216|A0A4R6F216_9BURK Methionine biosynthesis protein MetW OS=Paraburkholderia sp. BL10I2N1 OX=1938796 GN=B0G77_1481 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 10.06QPSVTRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.0RR14 pKa = 11.84THH16 pKa = 5.76GFRR19 pKa = 11.84VRR21 pKa = 11.84MKK23 pKa = 8.74TAGGRR28 pKa = 11.84KK29 pKa = 9.04VINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.65GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LAII44 pKa = 4.0

Molecular weight: 5.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7997

0

7997

2448043

29

3160

306.1

33.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.297 ± 0.04

1.003 ± 0.009

5.402 ± 0.021

5.186 ± 0.027

3.716 ± 0.017

8.109 ± 0.026

2.373 ± 0.013

4.755 ± 0.018

3.117 ± 0.023

10.131 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.401 ± 0.011

2.825 ± 0.021

5.123 ± 0.022

3.606 ± 0.015

7.078 ± 0.031

5.765 ± 0.022

5.508 ± 0.022

7.777 ± 0.021

1.388 ± 0.01

2.44 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski