Ichthyophthirius multifiliis (strain G5) (White spot disease agent) (Ich)

Taxonomy: cellular organisms; Eukaryota; Sar; Alveolata; Ciliophora; Intramacronucleata; Oligohymenophorea; Hymenostomatida; Ophryoglenina; Ichthyophthirius; Ichthyophthirius multifiliis

Average proteome isoelectric point is 7.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8049 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G0R2J4|G0R2J4_ICHMG Uncharacterized protein OS=Ichthyophthirius multifiliis (strain G5) OX=857967 GN=IMG5_178860 PE=4 SV=1
MM1 pKa = 6.81EE2 pKa = 4.6TEE4 pKa = 3.97INLNQIQQQEE14 pKa = 4.37EE15 pKa = 4.15EE16 pKa = 4.6GFYY19 pKa = 10.96ASYY22 pKa = 11.2EE23 pKa = 4.23EE24 pKa = 5.11YY25 pKa = 10.71FLDD28 pKa = 3.74CCRR31 pKa = 11.84FNDD34 pKa = 3.41IEE36 pKa = 4.24EE37 pKa = 4.29TKK39 pKa = 10.53FCIEE43 pKa = 4.21NGCDD47 pKa = 3.06ITWFDD52 pKa = 3.57NQINNGLHH60 pKa = 6.2WATINNQKK68 pKa = 10.6EE69 pKa = 4.11IVSYY73 pKa = 10.78LIEE76 pKa = 4.15TLKK79 pKa = 10.84VDD81 pKa = 4.73ANIQNSLGKK90 pKa = 8.33TAFDD94 pKa = 3.56EE95 pKa = 4.67AAQLNYY101 pKa = 10.96NEE103 pKa = 4.64IAQILAKK110 pKa = 8.83VTKK113 pKa = 9.59EE114 pKa = 4.32DD115 pKa = 3.25MSIYY119 pKa = 10.24QGLDD123 pKa = 2.99DD124 pKa = 4.67VQASDD129 pKa = 4.38EE130 pKa = 4.13NNEE133 pKa = 4.4DD134 pKa = 3.59KK135 pKa = 11.3EE136 pKa = 4.41EE137 pKa = 4.36TISNSS142 pKa = 3.0

Molecular weight: 16.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G0QSR6|G0QSR6_ICHMG Uncharacterized protein OS=Ichthyophthirius multifiliis (strain G5) OX=857967 GN=IMG5_103090 PE=4 SV=1
MM1 pKa = 7.62KK2 pKa = 10.27LFKK5 pKa = 10.55KK6 pKa = 10.3IIKK9 pKa = 6.55QQRR12 pKa = 11.84MYY14 pKa = 10.73LIKK17 pKa = 10.93VNNKK21 pKa = 9.3HH22 pKa = 6.58IKK24 pKa = 10.13IKK26 pKa = 10.73DD27 pKa = 3.39KK28 pKa = 11.45LKK30 pKa = 10.66IQINKK35 pKa = 9.48NKK37 pKa = 9.89FYY39 pKa = 10.8KK40 pKa = 10.06INKK43 pKa = 8.63NKK45 pKa = 9.93KK46 pKa = 7.2QSKK49 pKa = 6.71TRR51 pKa = 11.84LRR53 pKa = 11.84IRR55 pKa = 11.84IRR57 pKa = 11.84IRR59 pKa = 11.84IRR61 pKa = 11.84IRR63 pKa = 11.84IRR65 pKa = 11.84LRR67 pKa = 11.84IRR69 pKa = 11.84IRR71 pKa = 11.84IRR73 pKa = 11.84IRR75 pKa = 11.84IRR77 pKa = 11.84IRR79 pKa = 11.84IRR81 pKa = 11.84IRR83 pKa = 11.84IRR85 pKa = 11.84IRR87 pKa = 11.84IRR89 pKa = 11.84IRR91 pKa = 11.84IRR93 pKa = 11.84IRR95 pKa = 11.84IRR97 pKa = 11.84IRR99 pKa = 11.84IRR101 pKa = 11.84IRR103 pKa = 11.84IRR105 pKa = 11.84IRR107 pKa = 11.84IRR109 pKa = 11.84IRR111 pKa = 11.84IRR113 pKa = 11.84IRR115 pKa = 11.84IRR117 pKa = 11.84IRR119 pKa = 11.84IRR121 pKa = 11.84IRR123 pKa = 11.84IRR125 pKa = 11.84IRR127 pKa = 11.84IRR129 pKa = 11.84IRR131 pKa = 11.84IRR133 pKa = 11.84IRR135 pKa = 11.84IRR137 pKa = 11.84IRR139 pKa = 11.84IRR141 pKa = 11.84IRR143 pKa = 11.84LRR145 pKa = 11.84IRR147 pKa = 11.84IRR149 pKa = 11.84IRR151 pKa = 11.84IRR153 pKa = 11.84IRR155 pKa = 11.84IRR157 pKa = 11.84LRR159 pKa = 11.84RR160 pKa = 11.84RR161 pKa = 11.84IRR163 pKa = 11.84IRR165 pKa = 11.84IKK167 pKa = 8.84IRR169 pKa = 11.84IRR171 pKa = 11.84IRR173 pKa = 11.84IRR175 pKa = 11.84IRR177 pKa = 11.84IRR179 pKa = 11.84IKK181 pKa = 9.0IRR183 pKa = 11.84IRR185 pKa = 11.84IRR187 pKa = 11.84IRR189 pKa = 11.84IRR191 pKa = 11.84IRR193 pKa = 11.84IKK195 pKa = 9.0IRR197 pKa = 11.84IRR199 pKa = 11.84IRR201 pKa = 11.84IKK203 pKa = 10.6QKK205 pKa = 11.01LKK207 pKa = 11.08LKK209 pKa = 10.81LKK211 pKa = 10.52

Molecular weight: 27.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8049

0

8049

3335658

46

4916

414.4

48.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.257 ± 0.021

1.795 ± 0.035

4.828 ± 0.017

6.581 ± 0.036

5.72 ± 0.024

4.164 ± 0.023

1.531 ± 0.01

9.856 ± 0.035

9.846 ± 0.036

9.287 ± 0.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.991 ± 0.012

8.086 ± 0.033

2.612 ± 0.016

7.976 ± 0.038

2.896 ± 0.016

5.772 ± 0.02

3.941 ± 0.02

4.326 ± 0.02

0.81 ± 0.007

4.674 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski