Amino acid dipepetide frequency for Ichthyophthirius multifiliis (strain G5) (White spot disease agent) (Ich)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	1.479AlaAla: 1.479 ± 0.033	0.693AlaCys: 0.693 ± 0.018	1.544AlaAsp: 1.544 ± 0.023	1.872AlaGlu: 1.872 ± 0.026	2.001AlaPhe: 2.001 ± 0.021	1.587AlaGly: 1.587 ± 0.031	0.594AlaHis: 0.594 ± 0.015	2.974AlaIle: 2.974 ± 0.036	2.811AlaLys: 2.811 ± 0.037	3.476AlaLeu: 3.476 ± 0.047	0.619AlaMet: 0.619 ± 0.019	2.137AlaAsn: 2.137 ± 0.028	0.909AlaPro: 0.909 ± 0.019	2.194AlaGln: 2.194 ± 0.03	1.053AlaArg: 1.053 ± 0.019	1.977AlaSer: 1.977 ± 0.026	1.362AlaThr: 1.362 ± 0.027	1.646AlaVal: 1.646 ± 0.026	0.244AlaTrp: 0.244 ± 0.009	1.399AlaTyr: 1.399 ± 0.022	0.001AlaXaa: 0.001 ± 0.0
Cys	0.611CysAla: 0.611 ± 0.015	0.274CysCys: 0.274 ± 0.011	0.969CysAsp: 0.969 ± 0.028	1.034CysGlu: 1.034 ± 0.026	1.118CysPhe: 1.118 ± 0.021	0.939CysGly: 0.939 ± 0.021	0.313CysHis: 0.313 ± 0.015	1.707CysIle: 1.707 ± 0.037	1.438CysLys: 1.438 ± 0.04	1.874CysLeu: 1.874 ± 0.04	0.336CysMet: 0.336 ± 0.01	1.047CysAsn: 1.047 ± 0.033	0.735CysPro: 0.735 ± 0.03	1.208CysGln: 1.208 ± 0.032	0.481CysArg: 0.481 ± 0.013	1.247CysSer: 1.247 ± 0.038	0.853CysThr: 0.853 ± 0.044	0.94CysVal: 0.94 ± 0.024	0.132CysTrp: 0.132 ± 0.006	0.699CysTyr: 0.699 ± 0.017	0.0CysXaa: 0.0 ± 0.0
Asp	1.403AspAla: 1.403 ± 0.026	0.83AspCys: 0.83 ± 0.02	2.743AspAsp: 2.743 ± 0.05	3.717AspGlu: 3.717 ± 0.05	3.209AspPhe: 3.209 ± 0.033	2.182AspGly: 2.182 ± 0.041	0.616AspHis: 0.616 ± 0.017	4.781AspIle: 4.781 ± 0.046	4.151AspLys: 4.151 ± 0.038	4.727AspLeu: 4.727 ± 0.046	0.898AspMet: 0.898 ± 0.018	3.531AspAsn: 3.531 ± 0.041	1.417AspPro: 1.417 ± 0.024	3.523AspGln: 3.523 ± 0.04	1.225AspArg: 1.225 ± 0.022	2.704AspSer: 2.704 ± 0.032	1.685AspThr: 1.685 ± 0.027	2.143AspVal: 2.143 ± 0.027	0.458AspTrp: 0.458 ± 0.013	2.335AspTyr: 2.335 ± 0.029	0.001AspXaa: 0.001 ± 0.0
Glu	2.109GluAla: 2.109 ± 0.036	1.126GluCys: 1.126 ± 0.033	3.589GluAsp: 3.589 ± 0.044	6.143GluGlu: 6.143 ± 0.105	3.153GluPhe: 3.153 ± 0.036	2.621GluGly: 2.621 ± 0.037	0.816GluHis: 0.816 ± 0.017	6.481GluIle: 6.481 ± 0.052	7.403GluLys: 7.403 ± 0.074	5.224GluLeu: 5.224 ± 0.052	1.271GluMet: 1.271 ± 0.022	6.531GluAsn: 6.531 ± 0.056	1.016GluPro: 1.016 ± 0.023	4.918GluGln: 4.918 ± 0.077	1.914GluArg: 1.914 ± 0.031	3.056GluSer: 3.056 ± 0.033	2.283GluThr: 2.283 ± 0.025	2.669GluVal: 2.669 ± 0.031	0.565GluTrp: 0.565 ± 0.016	2.916GluTyr: 2.916 ± 0.028	0.001GluXaa: 0.001 ± 0.0
Phe	1.831PheAla: 1.831 ± 0.026	1.09PheCys: 1.09 ± 0.022	2.789PheAsp: 2.789 ± 0.038	3.272PheGlu: 3.272 ± 0.032	4.015PhePhe: 4.015 ± 0.059	2.667PheGly: 2.667 ± 0.036	0.859PheHis: 0.859 ± 0.017	5.407PheIle: 5.407 ± 0.057	4.733PheLys: 4.733 ± 0.043	6.038PheLeu: 6.038 ± 0.067	1.125PheMet: 1.125 ± 0.018	3.796PheAsn: 3.796 ± 0.035	1.503PhePro: 1.503 ± 0.026	4.54PheGln: 4.54 ± 0.044	1.498PheArg: 1.498 ± 0.024	3.834PheSer: 3.834 ± 0.039	2.21PheThr: 2.21 ± 0.026	2.408PheVal: 2.408 ± 0.03	0.524PheTrp: 0.524 ± 0.014	3.059PheTyr: 3.059 ± 0.034	0.002PheXaa: 0.002 ± 0.001
Gly	1.567GlyAla: 1.567 ± 0.029	0.817GlyCys: 0.817 ± 0.019	2.083GlyAsp: 2.083 ± 0.033	2.511GlyGlu: 2.511 ± 0.037	2.538GlyPhe: 2.538 ± 0.029	2.384GlyGly: 2.384 ± 0.049	0.781GlyHis: 0.781 ± 0.018	3.898GlyIle: 3.898 ± 0.036	3.956GlyLys: 3.956 ± 0.048	3.611GlyLeu: 3.611 ± 0.038	0.822GlyMet: 0.822 ± 0.02	2.857GlyAsn: 2.857 ± 0.034	0.955GlyPro: 0.955 ± 0.022	2.64GlyGln: 2.64 ± 0.034	1.314GlyArg: 1.314 ± 0.026	2.456GlySer: 2.456 ± 0.037	1.9GlyThr: 1.9 ± 0.032	2.153GlyVal: 2.153 ± 0.028	0.396GlyTrp: 0.396 ± 0.014	1.999GlyTyr: 1.999 ± 0.029	0.0GlyXaa: 0.0 ± 0.0
His	0.489HisAla: 0.489 ± 0.012	0.24HisCys: 0.24 ± 0.008	0.597HisAsp: 0.597 ± 0.014	0.867HisGlu: 0.867 ± 0.02	0.956HisPhe: 0.956 ± 0.019	0.742HisGly: 0.742 ± 0.019	0.306HisHis: 0.306 ± 0.013	1.497HisIle: 1.497 ± 0.02	1.323HisLys: 1.323 ± 0.029	1.618HisLeu: 1.618 ± 0.027	0.303HisMet: 0.303 ± 0.01	1.005HisAsn: 1.005 ± 0.018	0.635HisPro: 0.635 ± 0.014	1.101HisGln: 1.101 ± 0.019	0.544HisArg: 0.544 ± 0.013	0.956HisSer: 0.956 ± 0.016	0.616HisThr: 0.616 ± 0.014	0.663HisVal: 0.663 ± 0.014	0.139HisTrp: 0.139 ± 0.006	0.709HisTyr: 0.709 ± 0.014	0.0HisXaa: 0.0 ± 0.0
Ile	2.935IleAla: 2.935 ± 0.034	1.79IleCys: 1.79 ± 0.032	4.54IleAsp: 4.54 ± 0.041	5.731IleGlu: 5.731 ± 0.051	6.045IlePhe: 6.045 ± 0.066	3.536IleGly: 3.536 ± 0.035	1.565IleHis: 1.565 ± 0.02	10.099IleIle: 10.099 ± 0.094	9.486IleLys: 9.486 ± 0.075	9.565IleLeu: 9.565 ± 0.068	1.921IleMet: 1.921 ± 0.029	7.714IleAsn: 7.714 ± 0.056	2.77IlePro: 2.77 ± 0.031	8.316IleGln: 8.316 ± 0.058	2.791IleArg: 2.791 ± 0.042	5.917IleSer: 5.917 ± 0.046	3.637IleThr: 3.637 ± 0.039	4.114IleVal: 4.114 ± 0.065	0.828IleTrp: 0.828 ± 0.015	5.254IleTyr: 5.254 ± 0.065	0.004IleXaa: 0.004 ± 0.001
Lys	2.996LysAla: 2.996 ± 0.041	1.738LysCys: 1.738 ± 0.058	4.758LysAsp: 4.758 ± 0.054	6.82LysGlu: 6.82 ± 0.06	4.231LysPhe: 4.231 ± 0.037	3.604LysGly: 3.604 ± 0.037	1.386LysHis: 1.386 ± 0.031	10.155LysIle: 10.155 ± 0.078	11.548LysLys: 11.548 ± 0.119	7.951LysLeu: 7.951 ± 0.057	2.248LysMet: 2.248 ± 0.029	9.131LysAsn: 9.131 ± 0.073	2.122LysPro: 2.122 ± 0.036	8.345LysGln: 8.345 ± 0.074	2.913LysArg: 2.913 ± 0.037	4.986LysSer: 4.986 ± 0.036	4.0LysThr: 4.0 ± 0.037	3.994LysVal: 3.994 ± 0.045	0.726LysTrp: 0.726 ± 0.018	4.81LysTyr: 4.81 ± 0.046	0.001LysXaa: 0.001 ± 0.001
Leu	3.286LeuAla: 3.286 ± 0.041	1.542LeuCys: 1.542 ± 0.024	4.394LeuAsp: 4.394 ± 0.041	6.013LeuGlu: 6.013 ± 0.057	5.334LeuPhe: 5.334 ± 0.058	3.761LeuGly: 3.761 ± 0.037	1.53LeuHis: 1.53 ± 0.022	8.796LeuIle: 8.796 ± 0.067	9.75LeuLys: 9.75 ± 0.072	8.72LeuLeu: 8.72 ± 0.082	1.9LeuMet: 1.9 ± 0.031	7.419LeuAsn: 7.419 ± 0.059	2.385LeuPro: 2.385 ± 0.037	7.133LeuGln: 7.133 ± 0.052	2.96LeuArg: 2.96 ± 0.034	5.438LeuSer: 5.438 ± 0.049	3.665LeuThr: 3.665 ± 0.038	4.08LeuVal: 4.08 ± 0.049	0.702LeuTrp: 0.702 ± 0.017	4.061LeuTyr: 4.061 ± 0.035	0.002LeuXaa: 0.002 ± 0.001
Met	0.812MetAla: 0.812 ± 0.017	0.327MetCys: 0.327 ± 0.011	0.987MetAsp: 0.987 ± 0.019	1.428MetGlu: 1.428 ± 0.021	0.959MetPhe: 0.959 ± 0.016	0.868MetGly: 0.868 ± 0.019	0.404MetHis: 0.404 ± 0.011	1.869MetIle: 1.869 ± 0.028	2.139MetLys: 2.139 ± 0.029	1.684MetLeu: 1.684 ± 0.021	0.471MetMet: 0.471 ± 0.013	1.589MetAsn: 1.589 ± 0.022	0.593MetPro: 0.593 ± 0.015	1.385MetGln: 1.385 ± 0.024	0.663MetArg: 0.663 ± 0.016	1.169MetSer: 1.169 ± 0.018	0.787MetThr: 0.787 ± 0.02	0.9MetVal: 0.9 ± 0.018	0.154MetTrp: 0.154 ± 0.005	0.722MetTyr: 0.722 ± 0.015	0.0MetXaa: 0.0 ± 0.0
Asn	2.245AsnAla: 2.245 ± 0.029	1.304AsnCys: 1.304 ± 0.036	3.586AsnAsp: 3.586 ± 0.045	5.105AsnGlu: 5.105 ± 0.045	4.278AsnPhe: 4.278 ± 0.038	2.893AsnGly: 2.893 ± 0.046	1.116AsnHis: 1.116 ± 0.02	8.506AsnIle: 8.506 ± 0.066	7.723AsnLys: 7.723 ± 0.068	7.426AsnLeu: 7.426 ± 0.052	1.644AsnMet: 1.644 ± 0.023	8.06AsnAsn: 8.06 ± 0.117	2.283AsnPro: 2.283 ± 0.033	7.292AsnGln: 7.292 ± 0.063	1.968AsnArg: 1.968 ± 0.027	4.706AsnSer: 4.706 ± 0.04	3.274AsnThr: 3.274 ± 0.037	3.085AsnVal: 3.085 ± 0.03	0.55AsnTrp: 0.55 ± 0.013	3.811AsnTyr: 3.811 ± 0.036	0.001AsnXaa: 0.001 ± 0.001
Pro	0.829ProAla: 0.829 ± 0.019	0.403ProCys: 0.403 ± 0.013	1.33ProAsp: 1.33 ± 0.021	2.009ProGlu: 2.009 ± 0.032	1.577ProPhe: 1.577 ± 0.024	1.111ProGly: 1.111 ± 0.028	0.447ProHis: 0.447 ± 0.013	2.217ProIle: 2.217 ± 0.028	2.277ProLys: 2.277 ± 0.035	2.481ProLeu: 2.481 ± 0.035	0.35ProMet: 0.35 ± 0.011	1.938ProAsn: 1.938 ± 0.029	1.047ProPro: 1.047 ± 0.038	2.093ProGln: 2.093 ± 0.03	0.647ProArg: 0.647 ± 0.016	1.631ProSer: 1.631 ± 0.028	1.12ProThr: 1.12 ± 0.033	1.194ProVal: 1.194 ± 0.023	0.225ProTrp: 0.225 ± 0.007	1.194ProTyr: 1.194 ± 0.022	0.0ProXaa: 0.0 ± 0.0
Gln	2.207GlnAla: 2.207 ± 0.029	1.323GlnCys: 1.323 ± 0.069	3.387GlnAsp: 3.387 ± 0.036	5.644GlnGlu: 5.644 ± 0.081	3.834GlnPhe: 3.834 ± 0.033	2.709GlnGly: 2.709 ± 0.035	0.931GlnHis: 0.931 ± 0.015	8.359GlnIle: 8.359 ± 0.064	9.404GlnLys: 9.404 ± 0.08	6.304GlnLeu: 6.304 ± 0.047	1.691GlnMet: 1.691 ± 0.022	7.887GlnAsn: 7.887 ± 0.063	1.434GlnPro: 1.434 ± 0.03	8.679GlnGln: 8.679 ± 0.152	2.111GlnArg: 2.111 ± 0.027	3.867GlnSer: 3.867 ± 0.036	2.974GlnThr: 2.974 ± 0.033	2.942GlnVal: 2.942 ± 0.035	0.558GlnTrp: 0.558 ± 0.016	3.511GlnTyr: 3.511 ± 0.036	0.001GlnXaa: 0.001 ± 0.001
Arg	1.064ArgAla: 1.064 ± 0.02	0.48ArgCys: 0.48 ± 0.013	1.484ArgAsp: 1.484 ± 0.022	1.922ArgGlu: 1.922 ± 0.027	1.524ArgPhe: 1.524 ± 0.025	1.162ArgGly: 1.162 ± 0.022	0.493ArgHis: 0.493 ± 0.014	2.985ArgIle: 2.985 ± 0.041	3.05ArgLys: 3.05 ± 0.04	2.595ArgLeu: 2.595 ± 0.029	0.615ArgMet: 0.615 ± 0.013	2.195ArgAsn: 2.195 ± 0.029	0.785ArgPro: 0.785 ± 0.017	1.887ArgGln: 1.887 ± 0.025	1.14ArgArg: 1.14 ± 0.024	1.497ArgSer: 1.497 ± 0.023	1.2ArgThr: 1.2 ± 0.02	1.448ArgVal: 1.448 ± 0.024	0.234ArgTrp: 0.234 ± 0.009	1.2ArgTyr: 1.2 ± 0.02	0.0ArgXaa: 0.0 ± 0.0
Ser	1.896SerAla: 1.896 ± 0.026	1.186SerCys: 1.186 ± 0.043	2.707SerAsp: 2.707 ± 0.031	3.427SerGlu: 3.427 ± 0.039	3.716SerPhe: 3.716 ± 0.039	2.512SerGly: 2.512 ± 0.035	0.957SerHis: 0.957 ± 0.018	5.421SerIle: 5.421 ± 0.04	5.121SerLys: 5.121 ± 0.039	5.868SerLeu: 5.868 ± 0.05	0.991SerMet: 0.991 ± 0.019	4.214SerAsn: 4.214 ± 0.039	1.566SerPro: 1.566 ± 0.024	4.496SerGln: 4.496 ± 0.043	1.695SerArg: 1.695 ± 0.027	3.939SerSer: 3.939 ± 0.043	2.443SerThr: 2.443 ± 0.029	2.603SerVal: 2.603 ± 0.03	0.436SerTrp: 0.436 ± 0.013	2.524SerTyr: 2.524 ± 0.03	0.001SerXaa: 0.001 ± 0.0
Thr	1.458ThrAla: 1.458 ± 0.026	0.966ThrCys: 0.966 ± 0.042	1.763ThrAsp: 1.763 ± 0.023	2.28ThrGlu: 2.28 ± 0.03	2.287ThrPhe: 2.287 ± 0.029	2.078ThrGly: 2.078 ± 0.038	0.68ThrHis: 0.68 ± 0.015	3.705ThrIle: 3.705 ± 0.041	3.449ThrLys: 3.449 ± 0.031	3.883ThrLeu: 3.883 ± 0.038	0.686ThrMet: 0.686 ± 0.018	2.824ThrAsn: 2.824 ± 0.038	1.313ThrPro: 1.313 ± 0.02	2.942ThrGln: 2.942 ± 0.032	1.094ThrArg: 1.094 ± 0.02	2.42ThrSer: 2.42 ± 0.029	1.662ThrThr: 1.662 ± 0.026	1.843ThrVal: 1.843 ± 0.027	0.314ThrTrp: 0.314 ± 0.01	1.76ThrTyr: 1.76 ± 0.023	0.001ThrXaa: 0.001 ± 0.0
Val	1.579ValAla: 1.579 ± 0.027	0.863ValCys: 0.863 ± 0.021	2.242ValAsp: 2.242 ± 0.03	2.556ValGlu: 2.556 ± 0.035	2.763ValPhe: 2.763 ± 0.031	1.981ValGly: 1.981 ± 0.032	0.725ValHis: 0.725 ± 0.015	3.857ValIle: 3.857 ± 0.057	3.835ValLys: 3.835 ± 0.037	4.389ValLeu: 4.389 ± 0.042	0.84ValMet: 0.84 ± 0.018	3.023ValAsn: 3.023 ± 0.032	1.298ValPro: 1.298 ± 0.024	3.1ValGln: 3.1 ± 0.036	1.353ValArg: 1.353 ± 0.024	2.656ValSer: 2.656 ± 0.024	1.589ValThr: 1.589 ± 0.026	2.072ValVal: 2.072 ± 0.027	0.456ValTrp: 0.456 ± 0.015	2.081ValTyr: 2.081 ± 0.026	0.0ValXaa: 0.0 ± 0.0
Trp	0.353TrpAla: 0.353 ± 0.012	0.128TrpCys: 0.128 ± 0.007	0.531TrpAsp: 0.531 ± 0.016	0.556TrpGlu: 0.556 ± 0.014	0.355TrpPhe: 0.355 ± 0.01	0.381TrpGly: 0.381 ± 0.014	0.126TrpHis: 0.126 ± 0.006	0.772TrpIle: 0.772 ± 0.018	0.841TrpLys: 0.841 ± 0.018	0.608TrpLeu: 0.608 ± 0.014	0.166TrpMet: 0.166 ± 0.007	0.642TrpAsn: 0.642 ± 0.015	0.219TrpPro: 0.219 ± 0.008	0.419TrpGln: 0.419 ± 0.012	0.299TrpArg: 0.299 ± 0.011	0.566TrpSer: 0.566 ± 0.014	0.334TrpThr: 0.334 ± 0.011	0.444TrpVal: 0.444 ± 0.013	0.079TrpTrp: 0.079 ± 0.005	0.283TrpTyr: 0.283 ± 0.009	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.421TyrAla: 1.421 ± 0.025	0.833TyrCys: 0.833 ± 0.017	2.259TyrAsp: 2.259 ± 0.027	2.897TyrGlu: 2.897 ± 0.031	3.304TyrPhe: 3.304 ± 0.046	1.92TyrGly: 1.92 ± 0.026	0.662TyrHis: 0.662 ± 0.017	5.066TyrIle: 5.066 ± 0.061	4.026TyrLys: 4.026 ± 0.037	4.827TyrLeu: 4.827 ± 0.042	1.016TyrMet: 1.016 ± 0.019	3.316TyrAsn: 3.316 ± 0.038	1.134TyrPro: 1.134 ± 0.02	3.543TyrGln: 3.543 ± 0.034	1.295TyrArg: 1.295 ± 0.021	2.697TyrSer: 2.697 ± 0.03	1.811TyrThr: 1.811 ± 0.026	1.917TyrVal: 1.917 ± 0.021	0.383TyrTrp: 0.383 ± 0.012	2.416TyrTyr: 2.416 ± 0.037	0.002TyrXaa: 0.002 ± 0.001
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.002XaaPhe: 0.002 ± 0.001	0.001XaaGly: 0.001 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.004XaaIle: 0.004 ± 0.001	0.001XaaLys: 0.001 ± 0.001	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.001XaaAsn: 0.001 ± 0.001	0.0XaaPro: 0.0 ± 0.0	0.001XaaGln: 0.001 ± 0.0	0.001XaaArg: 0.001 ± 0.001	0.001XaaSer: 0.001 ± 0.001	0.001XaaThr: 0.001 ± 0.001	0.001XaaVal: 0.001 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.001XaaTyr: 0.001 ± 0.001	0.51XaaXaa: 0.51 ± 0.129

Statistics based on 8049 proteins (3335658 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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