Perkinsela sp. CCAP 1560/4

Taxonomy: cellular organisms; Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Prokinetoplastina; Ichthyobodonidae; Perkinsela; unclassified Perkinsela

Average proteome isoelectric point is 7.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4883 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0L1KSU0|A0A0L1KSU0_9EUGL Developmentally regulated GTP-binding protein OS=Perkinsela sp. CCAP 1560/4 OX=1314962 GN=XU18_2431 PE=4 SV=1
MM1 pKa = 7.38YY2 pKa = 10.36SIDD5 pKa = 3.62PDD7 pKa = 4.23GIARR11 pKa = 11.84VEE13 pKa = 4.04PSVMDD18 pKa = 3.95DD19 pKa = 3.27QTLLEE24 pKa = 4.18LVVVGFDD31 pKa = 3.33GVEE34 pKa = 4.05VFQSSNGDD42 pKa = 3.21FLDD45 pKa = 3.05IHH47 pKa = 6.58RR48 pKa = 11.84WDD50 pKa = 3.63GVTVDD55 pKa = 3.58SAQNIHH61 pKa = 6.87SITFCGTEE69 pKa = 3.84DD70 pKa = 4.24RR71 pKa = 11.84LLDD74 pKa = 4.45SYY76 pKa = 12.07DD77 pKa = 3.9DD78 pKa = 4.58DD79 pKa = 4.96YY80 pKa = 11.81EE81 pKa = 5.34GNVEE85 pKa = 4.12PGGTIHH91 pKa = 7.69LDD93 pKa = 3.07WLPTHH98 pKa = 7.07IAA100 pKa = 3.41

Molecular weight: 11.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0L1KX96|A0A0L1KX96_9EUGL Uncharacterized protein (Fragment) OS=Perkinsela sp. CCAP 1560/4 OX=1314962 GN=XU18_0878 PE=4 SV=1
MM1 pKa = 8.0IEE3 pKa = 4.0VNEE6 pKa = 4.24TRR8 pKa = 11.84LKK10 pKa = 10.0VHH12 pKa = 6.34LNCRR16 pKa = 11.84IFSTGGGHH24 pKa = 5.72STTAINEE31 pKa = 4.04PPRR34 pKa = 11.84QIMEE38 pKa = 4.21EE39 pKa = 3.96LLQVLLHH46 pKa = 5.34SARR49 pKa = 11.84LRR51 pKa = 11.84FSGLLRR57 pKa = 11.84SRR59 pKa = 11.84QPANFVNPRR68 pKa = 11.84KK69 pKa = 10.4YY70 pKa = 10.31IVWCQFTQQGANPLRR85 pKa = 11.84RR86 pKa = 11.84GFHH89 pKa = 6.64DD90 pKa = 3.03RR91 pKa = 11.84WNPAEE96 pKa = 4.12LNRR99 pKa = 11.84LSWHH103 pKa = 6.04VRR105 pKa = 11.84SQHH108 pKa = 6.96LILKK112 pKa = 7.24YY113 pKa = 9.98SCIYY117 pKa = 10.65SLLEE121 pKa = 3.75HH122 pKa = 6.9AMFAAKK128 pKa = 10.2AGNAKK133 pKa = 10.26

Molecular weight: 15.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4883

0

4883

1753898

38

4342

359.2

40.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.886 ± 0.033

2.145 ± 0.02

5.108 ± 0.022

6.391 ± 0.033

4.413 ± 0.024

5.425 ± 0.031

2.954 ± 0.016

5.601 ± 0.027

5.655 ± 0.032

9.985 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.41 ± 0.014

4.073 ± 0.022

4.605 ± 0.027

3.896 ± 0.021

6.299 ± 0.032

8.565 ± 0.034

5.533 ± 0.021

6.117 ± 0.019

1.167 ± 0.011

2.768 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski