Amino acid dipepetide frequency for Perkinsela sp. CCAP 1560/4

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.026AlaAla: 5.026 ± 0.066	1.556AlaCys: 1.556 ± 0.031	3.87AlaAsp: 3.87 ± 0.052	4.298AlaGlu: 4.298 ± 0.056	3.134AlaPhe: 3.134 ± 0.052	3.352AlaGly: 3.352 ± 0.055	2.045AlaHis: 2.045 ± 0.039	3.835AlaIle: 3.835 ± 0.048	3.916AlaLys: 3.916 ± 0.056	7.877AlaLeu: 7.877 ± 0.072	1.795AlaMet: 1.795 ± 0.034	2.732AlaAsn: 2.732 ± 0.045	2.666AlaPro: 2.666 ± 0.04	2.648AlaGln: 2.648 ± 0.045	3.981AlaArg: 3.981 ± 0.053	5.662AlaSer: 5.662 ± 0.053	3.483AlaThr: 3.483 ± 0.051	4.42AlaVal: 4.42 ± 0.054	0.69AlaTrp: 0.69 ± 0.017	1.876AlaTyr: 1.876 ± 0.033	0.001AlaXaa: 0.001 ± 0.001
Cys	1.734CysAla: 1.734 ± 0.034	0.437CysCys: 0.437 ± 0.018	0.992CysAsp: 0.992 ± 0.027	1.232CysGlu: 1.232 ± 0.026	1.066CysPhe: 1.066 ± 0.025	1.602CysGly: 1.602 ± 0.033	0.669CysHis: 0.669 ± 0.019	1.305CysIle: 1.305 ± 0.029	0.859CysLys: 0.859 ± 0.025	2.16CysLeu: 2.16 ± 0.039	0.532CysMet: 0.532 ± 0.015	0.75CysAsn: 0.75 ± 0.021	1.011CysPro: 1.011 ± 0.031	0.704CysGln: 0.704 ± 0.022	1.183CysArg: 1.183 ± 0.027	1.695CysSer: 1.695 ± 0.035	1.269CysThr: 1.269 ± 0.031	1.511CysVal: 1.511 ± 0.033	0.221CysTrp: 0.221 ± 0.012	0.516CysTyr: 0.516 ± 0.015	0.001CysXaa: 0.001 ± 0.001
Asp	4.108AspAla: 4.108 ± 0.054	0.97AspCys: 0.97 ± 0.026	2.862AspAsp: 2.862 ± 0.05	3.689AspGlu: 3.689 ± 0.048	2.494AspPhe: 2.494 ± 0.034	3.348AspGly: 3.348 ± 0.051	1.16AspHis: 1.16 ± 0.021	3.322AspIle: 3.322 ± 0.053	2.239AspLys: 2.239 ± 0.039	5.18AspLeu: 5.18 ± 0.066	1.204AspMet: 1.204 ± 0.025	1.729AspAsn: 1.729 ± 0.043	2.4AspPro: 2.4 ± 0.04	1.408AspGln: 1.408 ± 0.03	2.85AspArg: 2.85 ± 0.049	4.011AspSer: 4.011 ± 0.054	2.63AspThr: 2.63 ± 0.041	3.433AspVal: 3.433 ± 0.048	0.648AspTrp: 0.648 ± 0.029	1.395AspTyr: 1.395 ± 0.029	0.001AspXaa: 0.001 ± 0.001
Glu	4.271GluAla: 4.271 ± 0.054	1.258GluCys: 1.258 ± 0.03	3.075GluAsp: 3.075 ± 0.055	4.52GluGlu: 4.52 ± 0.074	2.458GluPhe: 2.458 ± 0.036	3.096GluGly: 3.096 ± 0.036	1.542GluHis: 1.542 ± 0.027	4.275GluIle: 4.275 ± 0.05	5.068GluLys: 5.068 ± 0.076	5.767GluLeu: 5.767 ± 0.066	1.938GluMet: 1.938 ± 0.035	3.63GluAsn: 3.63 ± 0.056	1.941GluPro: 1.941 ± 0.034	1.891GluGln: 1.891 ± 0.034	3.879GluArg: 3.879 ± 0.059	5.266GluSer: 5.266 ± 0.065	3.318GluThr: 3.318 ± 0.052	3.766GluVal: 3.766 ± 0.047	1.071GluTrp: 1.071 ± 0.026	1.884GluTyr: 1.884 ± 0.039	0.001GluXaa: 0.001 ± 0.001
Phe	2.839PheAla: 2.839 ± 0.044	0.968PheCys: 0.968 ± 0.023	2.641PheAsp: 2.641 ± 0.036	2.598PheGlu: 2.598 ± 0.04	2.07PhePhe: 2.07 ± 0.04	2.524PheGly: 2.524 ± 0.041	1.594PheHis: 1.594 ± 0.028	2.228PheIle: 2.228 ± 0.034	1.469PheLys: 1.469 ± 0.032	4.824PheLeu: 4.824 ± 0.069	0.869PheMet: 0.869 ± 0.023	1.429PheAsn: 1.429 ± 0.037	2.225PhePro: 2.225 ± 0.041	1.817PheGln: 1.817 ± 0.032	2.595PheArg: 2.595 ± 0.043	4.112PheSer: 4.112 ± 0.056	2.746PheThr: 2.746 ± 0.047	2.66PheVal: 2.66 ± 0.037	0.469PheTrp: 0.469 ± 0.014	1.449PheTyr: 1.449 ± 0.045	0.003PheXaa: 0.003 ± 0.001
Gly	3.112GlyAla: 3.112 ± 0.049	1.004GlyCys: 1.004 ± 0.026	2.773GlyAsp: 2.773 ± 0.041	3.607GlyGlu: 3.607 ± 0.057	2.254GlyPhe: 2.254 ± 0.033	3.227GlyGly: 3.227 ± 0.063	1.424GlyHis: 1.424 ± 0.033	3.516GlyIle: 3.516 ± 0.051	3.923GlyLys: 3.923 ± 0.104	4.523GlyLeu: 4.523 ± 0.052	1.4GlyMet: 1.4 ± 0.027	2.459GlyAsn: 2.459 ± 0.043	1.751GlyPro: 1.751 ± 0.033	1.705GlyGln: 1.705 ± 0.042	3.359GlyArg: 3.359 ± 0.062	4.947GlySer: 4.947 ± 0.07	3.587GlyThr: 3.587 ± 0.051	3.57GlyVal: 3.57 ± 0.05	0.623GlyTrp: 0.623 ± 0.021	1.481GlyTyr: 1.481 ± 0.031	0.002GlyXaa: 0.002 ± 0.001
His	2.093HisAla: 2.093 ± 0.037	0.731HisCys: 0.731 ± 0.021	1.281HisAsp: 1.281 ± 0.027	1.637HisGlu: 1.637 ± 0.032	1.48HisPhe: 1.48 ± 0.027	1.785HisGly: 1.785 ± 0.035	0.907HisHis: 0.907 ± 0.027	1.722HisIle: 1.722 ± 0.03	1.266HisLys: 1.266 ± 0.03	3.156HisLeu: 3.156 ± 0.047	0.69HisMet: 0.69 ± 0.019	1.014HisAsn: 1.014 ± 0.023	1.496HisPro: 1.496 ± 0.032	1.109HisGln: 1.109 ± 0.029	2.136HisArg: 2.136 ± 0.039	2.448HisSer: 2.448 ± 0.045	1.661HisThr: 1.661 ± 0.038	1.762HisVal: 1.762 ± 0.032	0.377HisTrp: 0.377 ± 0.013	0.791HisTyr: 0.791 ± 0.023	0.002HisXaa: 0.002 ± 0.001
Ile	4.19IleAla: 4.19 ± 0.061	1.565IleCys: 1.565 ± 0.033	3.673IleAsp: 3.673 ± 0.051	3.896IleGlu: 3.896 ± 0.055	2.565IlePhe: 2.565 ± 0.04	3.07IleGly: 3.07 ± 0.046	1.847IleHis: 1.847 ± 0.033	2.761IleIle: 2.761 ± 0.04	2.369IleLys: 2.369 ± 0.032	5.32IleLeu: 5.32 ± 0.07	0.958IleMet: 0.958 ± 0.025	1.834IleAsn: 1.834 ± 0.03	2.849IlePro: 2.849 ± 0.041	2.437IleGln: 2.437 ± 0.039	3.662IleArg: 3.662 ± 0.048	4.977IleSer: 4.977 ± 0.059	2.761IleThr: 2.761 ± 0.038	3.36IleVal: 3.36 ± 0.041	0.534IleTrp: 0.534 ± 0.018	1.381IleTyr: 1.381 ± 0.029	0.001IleXaa: 0.001 ± 0.001
Lys	3.802LysAla: 3.802 ± 0.058	1.213LysCys: 1.213 ± 0.032	2.582LysAsp: 2.582 ± 0.036	3.863LysGlu: 3.863 ± 0.079	2.157LysPhe: 2.157 ± 0.032	2.953LysGly: 2.953 ± 0.043	1.635LysHis: 1.635 ± 0.031	3.232LysIle: 3.232 ± 0.048	4.09LysLys: 4.09 ± 0.071	5.512LysLeu: 5.512 ± 0.058	1.383LysMet: 1.383 ± 0.028	2.686LysAsn: 2.686 ± 0.043	2.266LysPro: 2.266 ± 0.041	2.198LysGln: 2.198 ± 0.053	3.713LysArg: 3.713 ± 0.049	4.538LysSer: 4.538 ± 0.06	3.048LysThr: 3.048 ± 0.05	3.243LysVal: 3.243 ± 0.04	0.638LysTrp: 0.638 ± 0.017	1.795LysTyr: 1.795 ± 0.037	0.002LysXaa: 0.002 ± 0.001
Leu	6.32LeuAla: 6.32 ± 0.064	2.331LeuCys: 2.331 ± 0.038	5.359LeuAsp: 5.359 ± 0.064	5.945LeuGlu: 5.945 ± 0.068	4.321LeuPhe: 4.321 ± 0.057	4.702LeuGly: 4.702 ± 0.053	3.437LeuHis: 3.437 ± 0.041	4.321LeuIle: 4.321 ± 0.06	4.938LeuLys: 4.938 ± 0.069	9.612LeuLeu: 9.612 ± 0.103	2.211LeuMet: 2.211 ± 0.034	3.841LeuAsn: 3.841 ± 0.051	6.844LeuPro: 6.844 ± 0.096	4.429LeuGln: 4.429 ± 0.057	6.856LeuArg: 6.856 ± 0.072	9.553LeuSer: 9.553 ± 0.087	5.621LeuThr: 5.621 ± 0.075	5.33LeuVal: 5.33 ± 0.045	1.141LeuTrp: 1.141 ± 0.031	2.74LeuTyr: 2.74 ± 0.044	0.002LeuXaa: 0.002 ± 0.001
Met	1.398MetAla: 1.398 ± 0.026	0.537MetCys: 0.537 ± 0.015	1.203MetAsp: 1.203 ± 0.026	2.061MetGlu: 2.061 ± 0.038	0.953MetPhe: 0.953 ± 0.023	1.14MetGly: 1.14 ± 0.023	0.836MetHis: 0.836 ± 0.023	1.124MetIle: 1.124 ± 0.024	1.771MetLys: 1.771 ± 0.032	2.323MetLeu: 2.323 ± 0.038	0.533MetMet: 0.533 ± 0.019	1.175MetAsn: 1.175 ± 0.026	0.966MetPro: 0.966 ± 0.024	1.172MetGln: 1.172 ± 0.031	1.684MetArg: 1.684 ± 0.033	1.807MetSer: 1.807 ± 0.034	1.232MetThr: 1.232 ± 0.027	1.227MetVal: 1.227 ± 0.025	0.274MetTrp: 0.274 ± 0.013	0.68MetTyr: 0.68 ± 0.024	0.001MetXaa: 0.001 ± 0.001
Asn	3.348AsnAla: 3.348 ± 0.046	0.782AsnCys: 0.782 ± 0.02	1.924AsnAsp: 1.924 ± 0.043	2.644AsnGlu: 2.644 ± 0.044	1.882AsnPhe: 1.882 ± 0.035	2.03AsnGly: 2.03 ± 0.037	1.083AsnHis: 1.083 ± 0.024	2.469AsnIle: 2.469 ± 0.041	2.071AsnLys: 2.071 ± 0.035	4.098AsnLeu: 4.098 ± 0.059	0.92AsnMet: 0.92 ± 0.022	1.666AsnAsn: 1.666 ± 0.041	1.925AsnPro: 1.925 ± 0.037	1.864AsnGln: 1.864 ± 0.05	2.551AsnArg: 2.551 ± 0.047	3.046AsnSer: 3.046 ± 0.049	2.37AsnThr: 2.37 ± 0.037	2.601AsnVal: 2.601 ± 0.036	0.403AsnTrp: 0.403 ± 0.014	1.049AsnTyr: 1.049 ± 0.024	0.001AsnXaa: 0.001 ± 0.001
Pro	3.223ProAla: 3.223 ± 0.076	0.816ProCys: 0.816 ± 0.022	2.177ProAsp: 2.177 ± 0.036	3.022ProGlu: 3.022 ± 0.049	1.965ProPhe: 1.965 ± 0.043	2.374ProGly: 2.374 ± 0.051	1.381ProHis: 1.381 ± 0.026	2.344ProIle: 2.344 ± 0.035	2.681ProLys: 2.681 ± 0.037	3.963ProLeu: 3.963 ± 0.05	1.044ProMet: 1.044 ± 0.024	1.716ProAsn: 1.716 ± 0.032	2.733ProPro: 2.733 ± 0.055	1.978ProGln: 1.978 ± 0.038	2.729ProArg: 2.729 ± 0.043	4.606ProSer: 4.606 ± 0.058	2.748ProThr: 2.748 ± 0.051	2.691ProVal: 2.691 ± 0.041	0.546ProTrp: 0.546 ± 0.023	1.312ProTyr: 1.312 ± 0.032	0.002ProXaa: 0.002 ± 0.001
Gln	2.743GlnAla: 2.743 ± 0.051	0.855GlnCys: 0.855 ± 0.023	1.494GlnAsp: 1.494 ± 0.032	2.336GlnGlu: 2.336 ± 0.04	1.658GlnPhe: 1.658 ± 0.03	1.791GlnGly: 1.791 ± 0.029	1.06GlnHis: 1.06 ± 0.025	2.016GlnIle: 2.016 ± 0.032	2.658GlnLys: 2.658 ± 0.046	4.06GlnLeu: 4.06 ± 0.053	0.98GlnMet: 0.98 ± 0.023	1.783GlnAsn: 1.783 ± 0.032	1.556GlnPro: 1.556 ± 0.039	1.778GlnGln: 1.778 ± 0.034	2.809GlnArg: 2.809 ± 0.059	3.448GlnSer: 3.448 ± 0.062	2.18GlnThr: 2.18 ± 0.044	2.034GlnVal: 2.034 ± 0.032	0.623GlnTrp: 0.623 ± 0.021	1.102GlnTyr: 1.102 ± 0.024	0.001GlnXaa: 0.001 ± 0.001
Arg	4.068ArgAla: 4.068 ± 0.055	1.353ArgCys: 1.353 ± 0.031	2.897ArgAsp: 2.897 ± 0.042	3.92ArgGlu: 3.92 ± 0.054	2.731ArgPhe: 2.731 ± 0.054	3.329ArgGly: 3.329 ± 0.08	1.884ArgHis: 1.884 ± 0.034	3.779ArgIle: 3.779 ± 0.044	4.326ArgLys: 4.326 ± 0.056	6.239ArgLeu: 6.239 ± 0.062	1.705ArgMet: 1.705 ± 0.029	2.707ArgAsn: 2.707 ± 0.037	2.441ArgPro: 2.441 ± 0.051	2.404ArgGln: 2.404 ± 0.048	4.446ArgArg: 4.446 ± 0.063	4.954ArgSer: 4.954 ± 0.054	3.337ArgThr: 3.337 ± 0.053	3.879ArgVal: 3.879 ± 0.046	0.848ArgTrp: 0.848 ± 0.023	1.74ArgTyr: 1.74 ± 0.032	0.001ArgXaa: 0.001 ± 0.0
Ser	6.019SerAla: 6.019 ± 0.056	1.543SerCys: 1.543 ± 0.027	3.798SerAsp: 3.798 ± 0.052	4.787SerGlu: 4.787 ± 0.059	3.83SerPhe: 3.83 ± 0.054	5.086SerGly: 5.086 ± 0.08	2.329SerHis: 2.329 ± 0.039	5.081SerIle: 5.081 ± 0.058	4.44SerLys: 4.44 ± 0.058	9.59SerLeu: 9.59 ± 0.095	2.421SerMet: 2.421 ± 0.037	3.307SerAsn: 3.307 ± 0.054	3.793SerPro: 3.793 ± 0.054	3.441SerGln: 3.441 ± 0.049	4.723SerArg: 4.723 ± 0.056	7.604SerSer: 7.604 ± 0.084	5.164SerThr: 5.164 ± 0.054	5.625SerVal: 5.625 ± 0.068	0.9SerTrp: 0.9 ± 0.023	2.171SerTyr: 2.171 ± 0.033	0.002SerXaa: 0.002 ± 0.001
Thr	3.835ThrAla: 3.835 ± 0.052	1.123ThrCys: 1.123 ± 0.029	2.617ThrAsp: 2.617 ± 0.039	3.345ThrGlu: 3.345 ± 0.044	2.299ThrPhe: 2.299 ± 0.036	3.547ThrGly: 3.547 ± 0.08	1.659ThrHis: 1.659 ± 0.039	3.249ThrIle: 3.249 ± 0.042	3.059ThrLys: 3.059 ± 0.046	5.708ThrLeu: 5.708 ± 0.052	1.201ThrMet: 1.201 ± 0.027	2.253ThrAsn: 2.253 ± 0.035	2.777ThrPro: 2.777 ± 0.043	2.171ThrGln: 2.171 ± 0.04	3.216ThrArg: 3.216 ± 0.04	4.824ThrSer: 4.824 ± 0.059	3.016ThrThr: 3.016 ± 0.046	3.611ThrVal: 3.611 ± 0.046	0.527ThrTrp: 0.527 ± 0.019	1.287ThrTyr: 1.287 ± 0.028	0.002ThrXaa: 0.002 ± 0.001
Val	3.985ValAla: 3.985 ± 0.054	1.512ValCys: 1.512 ± 0.03	3.897ValAsp: 3.897 ± 0.051	4.197ValGlu: 4.197 ± 0.058	2.862ValPhe: 2.862 ± 0.039	3.099ValGly: 3.099 ± 0.038	1.901ValHis: 1.901 ± 0.034	3.168ValIle: 3.168 ± 0.04	3.176ValLys: 3.176 ± 0.043	5.919ValLeu: 5.919 ± 0.067	1.329ValMet: 1.329 ± 0.027	2.391ValAsn: 2.391 ± 0.041	2.834ValPro: 2.834 ± 0.037	2.409ValGln: 2.409 ± 0.043	3.96ValArg: 3.96 ± 0.05	5.056ValSer: 5.056 ± 0.057	2.921ValThr: 2.921 ± 0.04	4.119ValVal: 4.119 ± 0.057	0.604ValTrp: 0.604 ± 0.019	1.833ValTyr: 1.833 ± 0.031	0.001ValXaa: 0.001 ± 0.001
Trp	0.579TrpAla: 0.579 ± 0.019	0.256TrpCys: 0.256 ± 0.013	0.572TrpAsp: 0.572 ± 0.02	0.738TrpGlu: 0.738 ± 0.023	0.41TrpPhe: 0.41 ± 0.017	0.61TrpGly: 0.61 ± 0.022	0.313TrpHis: 0.313 ± 0.014	0.702TrpIle: 0.702 ± 0.022	1.048TrpLys: 1.048 ± 0.025	1.064TrpLeu: 1.064 ± 0.024	0.361TrpMet: 0.361 ± 0.016	0.627TrpAsn: 0.627 ± 0.017	0.352TrpPro: 0.352 ± 0.013	0.424TrpGln: 0.424 ± 0.015	0.847TrpArg: 0.847 ± 0.024	0.905TrpSer: 0.905 ± 0.021	0.683TrpThr: 0.683 ± 0.022	0.618TrpVal: 0.618 ± 0.02	0.21TrpTrp: 0.21 ± 0.013	0.352TrpTyr: 0.352 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.165TyrAla: 2.165 ± 0.034	0.638TyrCys: 0.638 ± 0.016	1.393TyrAsp: 1.393 ± 0.03	1.576TyrGlu: 1.576 ± 0.028	1.538TyrPhe: 1.538 ± 0.031	1.58TyrGly: 1.58 ± 0.027	0.836TyrHis: 0.836 ± 0.022	1.558TyrIle: 1.558 ± 0.031	1.183TyrLys: 1.183 ± 0.029	2.957TyrLeu: 2.957 ± 0.043	0.62TyrMet: 0.62 ± 0.02	0.998TyrAsn: 0.998 ± 0.025	1.224TyrPro: 1.224 ± 0.026	0.977TyrGln: 0.977 ± 0.027	1.806TyrArg: 1.806 ± 0.049	2.193TyrSer: 2.193 ± 0.036	1.551TyrThr: 1.551 ± 0.031	1.714TyrVal: 1.714 ± 0.029	0.326TyrTrp: 0.326 ± 0.015	0.844TyrTyr: 0.844 ± 0.026	0.001TyrXaa: 0.001 ± 0.001
Xaa	0.002XaaAla: 0.002 ± 0.001	0.001XaaCys: 0.001 ± 0.001	0.001XaaAsp: 0.001 ± 0.001	0.001XaaGlu: 0.001 ± 0.001	0.002XaaPhe: 0.002 ± 0.001	0.001XaaGly: 0.001 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.001	0.001XaaLys: 0.001 ± 0.001	0.003XaaLeu: 0.003 ± 0.001	0.001XaaMet: 0.001 ± 0.001	0.001XaaAsn: 0.001 ± 0.001	0.002XaaPro: 0.002 ± 0.001	0.002XaaGln: 0.002 ± 0.001	0.002XaaArg: 0.002 ± 0.001	0.002XaaSer: 0.002 ± 0.001	0.001XaaThr: 0.001 ± 0.001	0.001XaaVal: 0.001 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.001XaaTyr: 0.001 ± 0.001	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4883 proteins (1753898 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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