Labrenzia alba
Average proteome isoelectric point is 6.06
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6265 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0M6ZV23|A0A0M6ZV23_9RHOB Bacterial regulatory proteins tetR family OS=Labrenzia alba OX=311410 GN=LA5096_00961 PE=4 SV=1
MM1 pKa = 7.52 TKK3 pKa = 10.35 PSFDD7 pKa = 3.74 PAFLSVDD14 pKa = 3.16 EE15 pKa = 4.69 TMPDD19 pKa = 3.43 PSTSTEE25 pKa = 3.83 SHH27 pKa = 6.86 AEE29 pKa = 3.96 NLDD32 pKa = 3.64 QASLPFFSLDD42 pKa = 3.68 EE43 pKa = 4.43 IADD46 pKa = 3.92 QLTDD50 pKa = 4.41 GYY52 pKa = 9.61 WLNEE56 pKa = 3.73 GGLRR60 pKa = 11.84 RR61 pKa = 11.84 AFDD64 pKa = 3.73 TSSSNQVSVNIDD76 pKa = 3.38 GLSEE80 pKa = 4.18 EE81 pKa = 4.65 GQSLAQRR88 pKa = 11.84 ALTEE92 pKa = 4.08 WSNVADD98 pKa = 3.97 LEE100 pKa = 4.32 FVYY103 pKa = 11.05 VSGAAQITFNDD114 pKa = 3.63 EE115 pKa = 3.7 NTNASATSQTSGGMILSSEE134 pKa = 4.28 VNIPLSYY141 pKa = 11.11 LEE143 pKa = 4.02 THH145 pKa = 5.92 GTGVPGFAYY154 pKa = 10.29 FMYY157 pKa = 10.39 LRR159 pKa = 11.84 EE160 pKa = 4.27 IGQALGLGHH169 pKa = 7.49 AGNYY173 pKa = 8.67 EE174 pKa = 3.81 DD175 pKa = 3.87 TATYY179 pKa = 10.46 GVDD182 pKa = 2.75 NHH184 pKa = 6.22 YY185 pKa = 11.57 ANDD188 pKa = 3.42 SWQSSVMSAFSQTDD202 pKa = 3.2 NTSIDD207 pKa = 3.01 ASFAYY212 pKa = 9.4 PVTPMIADD220 pKa = 3.84 ILAVQNLYY228 pKa = 11.09 GEE230 pKa = 4.58 PDD232 pKa = 4.08 YY233 pKa = 10.9 IYY235 pKa = 9.9 PDD237 pKa = 3.5 YY238 pKa = 10.91 RR239 pKa = 11.84 IFGANVYY246 pKa = 10.7 GDD248 pKa = 3.53 YY249 pKa = 10.94 NEE251 pKa = 4.42 RR252 pKa = 11.84 GFYY255 pKa = 10.16 EE256 pKa = 4.19 ISPDD260 pKa = 3.11 ATATIFDD267 pKa = 4.33 SGGEE271 pKa = 3.87 DD272 pKa = 4.22 LIRR275 pKa = 11.84 FLGYY279 pKa = 10.53 SGDD282 pKa = 3.62 QRR284 pKa = 11.84 IDD286 pKa = 3.5 LNQEE290 pKa = 3.61 ATSDD294 pKa = 3.75 FAGGKK299 pKa = 9.52 GNLVIARR306 pKa = 11.84 GAVIEE311 pKa = 4.17 RR312 pKa = 11.84 AYY314 pKa = 10.56 GGNGNDD320 pKa = 3.68 EE321 pKa = 4.77 IIGNSSDD328 pKa = 3.7 NLLVGYY334 pKa = 9.97 DD335 pKa = 4.03 GNDD338 pKa = 3.28 TLIGGEE344 pKa = 4.21 GDD346 pKa = 4.25 DD347 pKa = 6.22 FLSGWNGVDD356 pKa = 4.81 LMFGGPGDD364 pKa = 4.07 DD365 pKa = 4.01 TIMATNEE372 pKa = 3.76 DD373 pKa = 4.28 VFFKK377 pKa = 10.86 DD378 pKa = 4.06 GAPIDD383 pKa = 4.14 LGGPGYY389 pKa = 10.73 DD390 pKa = 2.97 RR391 pKa = 11.84 LIISLEE397 pKa = 4.36 SYY399 pKa = 10.98 FEE401 pKa = 4.16 TDD403 pKa = 2.76 SLSQFGIEE411 pKa = 4.47 HH412 pKa = 6.54 FWGGGGDD419 pKa = 3.8 DD420 pKa = 4.45 RR421 pKa = 11.84 AIGDD425 pKa = 4.88 DD426 pKa = 3.7 GTVDD430 pKa = 3.67 YY431 pKa = 10.58 ILEE434 pKa = 4.16 GRR436 pKa = 11.84 YY437 pKa = 10.65 GNDD440 pKa = 2.58 ILTGNAGNDD449 pKa = 3.48 TLLGGDD455 pKa = 3.58 GHH457 pKa = 7.28 DD458 pKa = 4.1 VLTGGAGNDD467 pKa = 3.75 FLRR470 pKa = 11.84 GGIWGYY476 pKa = 10.86 DD477 pKa = 3.08 QLFAQEE483 pKa = 4.83 GDD485 pKa = 3.5 DD486 pKa = 3.56 TVYY489 pKa = 10.59 YY490 pKa = 11.0 ASGKK494 pKa = 8.75 VFWDD498 pKa = 3.16 DD499 pKa = 3.82 HH500 pKa = 6.25 KK501 pKa = 11.17 PRR503 pKa = 11.84 DD504 pKa = 3.56 IGGSGFDD511 pKa = 3.28 TLMIEE516 pKa = 4.47 NGSKK520 pKa = 10.65 FNTVGLSWYY529 pKa = 10.1 GFEE532 pKa = 4.41 AFEE535 pKa = 4.6 GAEE538 pKa = 4.08 KK539 pKa = 10.02 TDD541 pKa = 3.09 RR542 pKa = 11.84 VYY544 pKa = 11.52 GNDD547 pKa = 2.92 ASVNYY552 pKa = 10.38 RR553 pKa = 11.84 MDD555 pKa = 3.78 GGGGDD560 pKa = 3.92 DD561 pKa = 4.82 DD562 pKa = 5.34 LRR564 pKa = 11.84 GSGGNDD570 pKa = 2.89 TLLGGEE576 pKa = 4.33 GTDD579 pKa = 3.61 ILFGKK584 pKa = 10.4 DD585 pKa = 3.49 GDD587 pKa = 4.63 DD588 pKa = 4.53 LLDD591 pKa = 4.92 PGPSTSGQQEE601 pKa = 4.31 LFGGDD606 pKa = 3.02 GSDD609 pKa = 3.58 TYY611 pKa = 10.94 RR612 pKa = 11.84 ISTDD616 pKa = 2.55 SGRR619 pKa = 11.84 TYY621 pKa = 10.35 IEE623 pKa = 3.85 EE624 pKa = 4.21 LFEE627 pKa = 4.15 QGSDD631 pKa = 3.41 SLVFEE636 pKa = 4.87 SLMPSDD642 pKa = 3.99 IEE644 pKa = 4.08 ATIVDD649 pKa = 4.17 GDD651 pKa = 3.89 MLLLSWHH658 pKa = 7.39 DD659 pKa = 3.39 GTGQVTVNDD668 pKa = 4.35 LGQNIEE674 pKa = 4.18 RR675 pKa = 11.84 YY676 pKa = 9.35 EE677 pKa = 4.04 FADD680 pKa = 4.25 GSAYY684 pKa = 10.7 SADD687 pKa = 3.26 EE688 pKa = 4.45 FEE690 pKa = 5.06 FSS692 pKa = 3.85
Molecular weight: 74.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.697
IPC2_protein 3.617
IPC_protein 3.668
Toseland 3.427
ProMoST 3.821
Dawson 3.656
Bjellqvist 3.808
Wikipedia 3.605
Rodwell 3.478
Grimsley 3.338
Solomon 3.656
Lehninger 3.617
Nozaki 3.77
DTASelect 4.037
Thurlkill 3.478
EMBOSS 3.605
Sillero 3.783
Patrickios 1.087
IPC_peptide 3.656
IPC2_peptide 3.757
IPC2.peptide.svr19 3.732
Protein with the highest isoelectric point:
>tr|A0A0M6Z5K9|A0A0M6Z5K9_9RHOB L-threonine aldolase OS=Labrenzia alba OX=311410 GN=ltaE PE=3 SV=1
MM1 pKa = 7.68 KK2 pKa = 10.17 IKK4 pKa = 10.8 NSLKK8 pKa = 10.5 ALMNRR13 pKa = 11.84 HH14 pKa = 6.1 RR15 pKa = 11.84 DD16 pKa = 3.08 NRR18 pKa = 11.84 MVRR21 pKa = 11.84 RR22 pKa = 11.84 KK23 pKa = 10.31 GRR25 pKa = 11.84 VFIINKK31 pKa = 8.97 KK32 pKa = 8.7 NPRR35 pKa = 11.84 FKK37 pKa = 10.85 ARR39 pKa = 11.84 QGG41 pKa = 3.28
Molecular weight: 5.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.353
IPC2_protein 10.76
IPC_protein 12.325
Toseland 12.501
ProMoST 12.983
Dawson 12.501
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.31
Grimsley 12.53
Solomon 12.983
Lehninger 12.881
Nozaki 12.501
DTASelect 12.486
Thurlkill 12.501
EMBOSS 12.983
Sillero 12.501
Patrickios 12.047
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.053
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6265
0
6265
2028616
29
6892
323.8
35.35
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.755 ± 0.038
0.946 ± 0.01
5.994 ± 0.03
6.148 ± 0.032
4.132 ± 0.025
8.331 ± 0.033
2.018 ± 0.015
5.508 ± 0.02
3.746 ± 0.021
10.143 ± 0.037
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.514 ± 0.016
3.113 ± 0.019
4.773 ± 0.023
3.21 ± 0.017
6.16 ± 0.035
6.075 ± 0.024
5.444 ± 0.02
7.362 ± 0.022
1.321 ± 0.014
2.307 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here