halophilic archaeon

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales; unclassified Halobacteriales

Average proteome isoelectric point is 5.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5207 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A365TG49|A0A365TG49_9EURY Uncharacterized protein OS=halophilic archaeon OX=29295 GN=DMJ13_05755 PE=4 SV=1
MM1 pKa = 6.74TAPWNDD7 pKa = 2.82WNHH10 pKa = 5.35IVKK13 pKa = 10.43LDD15 pKa = 3.72PDD17 pKa = 3.52KK18 pKa = 11.18TLVEE22 pKa = 4.48GEE24 pKa = 4.34TFEE27 pKa = 5.39DD28 pKa = 3.95VCEE31 pKa = 4.26TGTDD35 pKa = 3.31ALEE38 pKa = 4.22IGGTLDD44 pKa = 3.19MTADD48 pKa = 3.09KK49 pKa = 9.11MQRR52 pKa = 11.84VIDD55 pKa = 3.76ACAKK59 pKa = 9.8YY60 pKa = 10.15DD61 pKa = 3.5VPVYY65 pKa = 10.01QEE67 pKa = 3.87PSNPAVVVDD76 pKa = 5.2DD77 pKa = 4.9DD78 pKa = 5.12ALDD81 pKa = 4.62GYY83 pKa = 10.38LVPIVLNAGEE93 pKa = 4.31VSWITGAHH101 pKa = 6.14KK102 pKa = 9.61EE103 pKa = 3.76WARR106 pKa = 11.84IDD108 pKa = 3.84DD109 pKa = 4.1VDD111 pKa = 3.7WEE113 pKa = 4.45KK114 pKa = 8.46TTTEE118 pKa = 4.62AYY120 pKa = 10.81VVMNPDD126 pKa = 3.26ASVADD131 pKa = 3.88LTGANCDD138 pKa = 3.49QTPEE142 pKa = 4.04DD143 pKa = 3.4VAAYY147 pKa = 10.49AEE149 pKa = 4.53IAEE152 pKa = 4.36HH153 pKa = 6.38MLGQEE158 pKa = 3.82IVYY161 pKa = 10.05VEE163 pKa = 3.9YY164 pKa = 10.91SGTFGDD170 pKa = 4.22PEE172 pKa = 4.48VVGAAQDD179 pKa = 3.92ALDD182 pKa = 4.47EE183 pKa = 4.42ATLFYY188 pKa = 11.13GGGIRR193 pKa = 11.84DD194 pKa = 3.94YY195 pKa = 11.35EE196 pKa = 4.48SAHH199 pKa = 5.93TMAQYY204 pKa = 11.54ADD206 pKa = 3.79TVVVGDD212 pKa = 4.2LVHH215 pKa = 7.49DD216 pKa = 4.38EE217 pKa = 4.4GVDD220 pKa = 3.55AVRR223 pKa = 11.84EE224 pKa = 4.29TVEE227 pKa = 4.04GARR230 pKa = 11.84DD231 pKa = 3.53ATHH234 pKa = 6.66AQAEE238 pKa = 4.68SAA240 pKa = 4.1

Molecular weight: 25.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A365TI33|A0A365TI33_9EURY 5 10-methylene tetrahydromethanopterin reductase OS=halophilic archaeon OX=29295 GN=DMJ13_09085 PE=3 SV=1
MM1 pKa = 7.07TRR3 pKa = 11.84RR4 pKa = 11.84PRR6 pKa = 11.84RR7 pKa = 11.84PRR9 pKa = 11.84RR10 pKa = 11.84PRR12 pKa = 11.84KK13 pKa = 8.54RR14 pKa = 11.84RR15 pKa = 11.84LRR17 pKa = 11.84RR18 pKa = 11.84RR19 pKa = 11.84TRR21 pKa = 11.84RR22 pKa = 11.84RR23 pKa = 11.84PRR25 pKa = 11.84RR26 pKa = 11.84RR27 pKa = 11.84TRR29 pKa = 11.84PRR31 pKa = 11.84HH32 pKa = 5.41PSPATSTRR40 pKa = 11.84CRR42 pKa = 11.84SGKK45 pKa = 10.12SGSNSSTSARR55 pKa = 11.84CPSRR59 pKa = 11.84ASPPGRR65 pKa = 11.84RR66 pKa = 11.84RR67 pKa = 11.84RR68 pKa = 11.84PPPNSPRR75 pKa = 11.84RR76 pKa = 11.84ATLPTSSTNWSSS88 pKa = 2.9

Molecular weight: 10.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5207

0

5207

1468061

22

1752

281.9

30.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.555 ± 0.051

0.714 ± 0.011

8.198 ± 0.044

8.108 ± 0.051

3.504 ± 0.024

8.333 ± 0.03

2.034 ± 0.019

3.781 ± 0.029

2.13 ± 0.026

8.952 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.831 ± 0.014

2.573 ± 0.022

4.634 ± 0.024

2.655 ± 0.025

6.632 ± 0.037

5.571 ± 0.03

6.465 ± 0.035

9.331 ± 0.037

1.219 ± 0.015

2.78 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski