Collinsella intestinalis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria;

Average proteome isoelectric point is 5.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1430 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A414FU40|A0A414FU40_9ACTN ArsC family transcriptional regulator OS=Collinsella intestinalis OX=147207 GN=spxA PE=3 SV=1
MM1 pKa = 7.24MFLAGCGPAPQGSGDD16 pKa = 3.86AADD19 pKa = 4.81GSASASGILLGDD31 pKa = 4.09GKK33 pKa = 7.94TLRR36 pKa = 11.84VGMEE40 pKa = 3.77AAYY43 pKa = 10.13APYY46 pKa = 8.99NWQVSEE52 pKa = 4.13EE53 pKa = 4.44SEE55 pKa = 4.02FTIPIEE61 pKa = 4.2NVPGAYY67 pKa = 9.89ADD69 pKa = 4.36GYY71 pKa = 10.23DD72 pKa = 3.51VQVAKK77 pKa = 10.6IIADD81 pKa = 3.79GLGMEE86 pKa = 4.56PVAVKK91 pKa = 10.9LEE93 pKa = 4.07FGSLIDD99 pKa = 4.24ALNNGQVDD107 pKa = 3.91IVCAGMSVTPEE118 pKa = 3.58RR119 pKa = 11.84AAAAAFSDD127 pKa = 4.49SYY129 pKa = 11.82VDD131 pKa = 4.55DD132 pKa = 6.16DD133 pKa = 3.91IVMICRR139 pKa = 11.84ADD141 pKa = 3.26SAYY144 pKa = 10.71AGATTFDD151 pKa = 3.87EE152 pKa = 4.71LADD155 pKa = 3.96ASILGQAATMYY166 pKa = 10.76DD167 pKa = 3.67DD168 pKa = 5.47VIAQIEE174 pKa = 4.79GANHH178 pKa = 4.7MTPAEE183 pKa = 4.14TQPMVVEE190 pKa = 4.02NLRR193 pKa = 11.84NGTCDD198 pKa = 3.01IITYY202 pKa = 11.01SMLSAPKK209 pKa = 10.1LLEE212 pKa = 4.09VYY214 pKa = 9.98PDD216 pKa = 3.72LVVLDD221 pKa = 4.45MEE223 pKa = 4.95DD224 pKa = 3.42AFEE227 pKa = 4.72GSLMPDD233 pKa = 2.93NAGVAKK239 pKa = 10.75GNDD242 pKa = 3.26AALDD246 pKa = 3.71KK247 pKa = 11.1VNEE250 pKa = 4.44IIASIDD256 pKa = 3.34DD257 pKa = 3.97EE258 pKa = 4.5EE259 pKa = 5.03RR260 pKa = 11.84QDD262 pKa = 3.31MWSACMDD269 pKa = 3.82RR270 pKa = 11.84QPAA273 pKa = 3.25

Molecular weight: 28.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A414NFS4|A0A414NFS4_9ACTN D-alanyl-lipoteichoic acid biosynthesis protein DltB OS=Collinsella intestinalis OX=147207 GN=dltB PE=3 SV=1
MM1 pKa = 7.38GALPGGGFMTEE12 pKa = 4.38EE13 pKa = 4.14KK14 pKa = 10.53QSTGLGDD21 pKa = 4.33GIARR25 pKa = 11.84VGDD28 pKa = 3.51IAQRR32 pKa = 11.84LCDD35 pKa = 3.35NRR37 pKa = 11.84RR38 pKa = 11.84FVIVSVCLLVFLVLLNRR55 pKa = 11.84VLAGEE60 pKa = 4.27IMALDD65 pKa = 4.16RR66 pKa = 11.84SAIALMVGHH75 pKa = 6.7VRR77 pKa = 11.84TAWLTPVMEE86 pKa = 4.56TVSFMVTPVPLFALIFAVALAGRR109 pKa = 11.84SRR111 pKa = 11.84GVGGLGWFCTINLAGSTILNQLLKK135 pKa = 10.52FAIQRR140 pKa = 11.84PRR142 pKa = 11.84PDD144 pKa = 2.76VSLRR148 pKa = 11.84LVEE151 pKa = 3.91IGGFSFPSGHH161 pKa = 6.22SMAAMAFFGLFIWLTWRR178 pKa = 11.84YY179 pKa = 9.56VRR181 pKa = 11.84DD182 pKa = 3.14RR183 pKa = 11.84RR184 pKa = 11.84ARR186 pKa = 11.84VGLTVFLCVMIAAVGFSRR204 pKa = 11.84VYY206 pKa = 10.87LGVHH210 pKa = 5.52YY211 pKa = 10.68ASDD214 pKa = 3.81VLGGFCASLIWLAVYY229 pKa = 8.62TKK231 pKa = 10.8VAGPTLAAAKK241 pKa = 10.11RR242 pKa = 11.84VGG244 pKa = 3.2

Molecular weight: 26.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1430

0

1430

497327

24

3859

347.8

37.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.637 ± 0.086

1.521 ± 0.03

6.282 ± 0.05

6.845 ± 0.07

3.667 ± 0.04

8.291 ± 0.055

1.969 ± 0.027

5.325 ± 0.056

3.549 ± 0.054

9.26 ± 0.072

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.841 ± 0.031

2.781 ± 0.043

4.364 ± 0.039

2.686 ± 0.028

6.338 ± 0.073

5.707 ± 0.043

5.234 ± 0.066

8.092 ± 0.056

0.959 ± 0.022

2.651 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski