Electrophorus electricus (Electric eel) (Gymnotus electricus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei; Osteoglossocephalai;

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 50899 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4W4DNE7|A0A4W4DNE7_ELEEL Uncharacterized protein OS=Electrophorus electricus OX=8005 GN=LOC113585447 PE=4 SV=1
MM1 pKa = 7.85LIPRR5 pKa = 11.84STKK8 pKa = 10.04HH9 pKa = 5.68IRR11 pKa = 11.84SILNNDD17 pKa = 2.96KK18 pKa = 10.8CYY20 pKa = 10.32KK21 pKa = 9.09XCVCVCVCVCVCVCVCVHH39 pKa = 6.99ACVQSTKK46 pKa = 10.38PPCLWKK52 pKa = 8.88QTDD55 pKa = 3.91AEE57 pKa = 4.21NMEE60 pKa = 4.31TPPRR64 pKa = 11.84QRR66 pKa = 11.84LKK68 pKa = 10.2TPDD71 pKa = 3.87PSVQNPSAQTQDD83 pKa = 3.86LRR85 pKa = 11.84VEE87 pKa = 4.53DD88 pKa = 5.24PSAQTSDD95 pKa = 3.68CSIEE99 pKa = 4.13NPSAQTQDD107 pKa = 3.39FRR109 pKa = 11.84VEE111 pKa = 4.21DD112 pKa = 4.5PSAQTSDD119 pKa = 3.69CSVEE123 pKa = 4.08NPSAQTQDD131 pKa = 3.42PSVQNPSAQTQDD143 pKa = 3.33PSVEE147 pKa = 4.1NPSAQTQDD155 pKa = 3.37HH156 pKa = 5.82RR157 pKa = 11.84VEE159 pKa = 4.81DD160 pKa = 4.76PSAQTPDD167 pKa = 3.88CSVQNPSAQTQDD179 pKa = 3.42PSVQNPSAQTQDD191 pKa = 3.36CSVEE195 pKa = 4.1NPSAQTQDD203 pKa = 3.41PRR205 pKa = 11.84VEE207 pKa = 4.48DD208 pKa = 4.5PSAQTSDD215 pKa = 4.7CIVLNPSDD223 pKa = 3.79QTQDD227 pKa = 3.2PSVEE231 pKa = 4.15NPSAQTPDD239 pKa = 3.65PSVEE243 pKa = 4.22TPSAQTPDD251 pKa = 3.76PSVEE255 pKa = 4.16TPSAQTQDD263 pKa = 3.65PSVEE267 pKa = 4.2TPNAQTQDD275 pKa = 3.39PSVEE279 pKa = 4.0NLSAQTQDD287 pKa = 3.34PSVEE291 pKa = 4.1NPSAQTQDD299 pKa = 3.35PSIKK303 pKa = 10.26RR304 pKa = 11.84QTNN307 pKa = 2.92

Molecular weight: 32.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4W4G318|A0A4W4G318_ELEEL G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Electrophorus electricus OX=8005 PE=3 SV=1
MM1 pKa = 7.42SSWASHH7 pKa = 3.85KK8 pKa = 8.13TFRR11 pKa = 11.84IKK13 pKa = 10.64RR14 pKa = 11.84FLAKK18 pKa = 9.71KK19 pKa = 9.58QKK21 pKa = 8.69QNRR24 pKa = 11.84PIPQWIRR31 pKa = 11.84MKK33 pKa = 9.89TGNKK37 pKa = 8.61IRR39 pKa = 11.84YY40 pKa = 7.09NSKK43 pKa = 8.3RR44 pKa = 11.84RR45 pKa = 11.84HH46 pKa = 3.95WRR48 pKa = 11.84RR49 pKa = 11.84TKK51 pKa = 10.83LGLL54 pKa = 3.67

Molecular weight: 6.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

22824

28075

50899

29296342

18

7019

575.6

64.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.677 ± 0.01

2.435 ± 0.008

5.105 ± 0.007

6.66 ± 0.013

3.91 ± 0.008

6.23 ± 0.011

2.734 ± 0.005

4.656 ± 0.009

5.554 ± 0.012

9.954 ± 0.013

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.457 ± 0.004

3.763 ± 0.007

5.312 ± 0.01

4.512 ± 0.009

5.553 ± 0.007

7.963 ± 0.012

5.509 ± 0.007

6.706 ± 0.009

1.244 ± 0.004

2.977 ± 0.006
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski