Sulfuracidifex tepidarius
Average proteome isoelectric point is 6.81
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2770 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A510E186|A0A510E186_9CREN Uncharacterized protein OS=Sulfuracidifex tepidarius OX=1294262 GN=IC006_0739 PE=4 SV=1
MM1 pKa = 7.37 MGLNTYY7 pKa = 7.84 TDD9 pKa = 4.15 PYY11 pKa = 10.49 EE12 pKa = 4.58 GNLDD16 pKa = 3.43 VGLGYY21 pKa = 7.96 TQNGRR26 pKa = 11.84 AFIVLTEE33 pKa = 3.96 GLPNDD38 pKa = 4.67 SITAFNVLKK47 pKa = 10.45 VIEE50 pKa = 4.29 MNAA53 pKa = 3.01
Molecular weight: 5.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.838
IPC2_protein 4.126
IPC_protein 3.846
Toseland 3.681
ProMoST 4.05
Dawson 3.846
Bjellqvist 4.012
Wikipedia 3.808
Rodwell 3.706
Grimsley 3.617
Solomon 3.795
Lehninger 3.757
Nozaki 4.012
DTASelect 4.126
Thurlkill 3.783
EMBOSS 3.821
Sillero 3.973
Patrickios 1.914
IPC_peptide 3.795
IPC2_peptide 3.935
IPC2.peptide.svr19 3.877
Protein with the highest isoelectric point:
>tr|A0A510DX84|A0A510DX84_9CREN Putative respiratory nitrate reductase subunit cytochrome b-561 OS=Sulfuracidifex tepidarius OX=1294262 GN=IC006_2151 PE=4 SV=1
MM1 pKa = 6.97 VRR3 pKa = 11.84 DD4 pKa = 3.7 YY5 pKa = 11.46 EE6 pKa = 4.22 RR7 pKa = 11.84 VEE9 pKa = 4.1 GLTLSKK15 pKa = 10.59 PRR17 pKa = 11.84 GEE19 pKa = 3.88 KK20 pKa = 9.54 RR21 pKa = 11.84 RR22 pKa = 11.84 GRR24 pKa = 11.84 EE25 pKa = 3.22 EE26 pKa = 4.0 YY27 pKa = 9.8 IRR29 pKa = 11.84 KK30 pKa = 9.35 KK31 pKa = 10.58 RR32 pKa = 11.84 ISNVSNNN39 pKa = 3.21
Molecular weight: 4.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.32
IPC2_protein 9.736
IPC_protein 10.599
Toseland 10.76
ProMoST 10.482
Dawson 10.847
Bjellqvist 10.57
Wikipedia 11.067
Rodwell 11.052
Grimsley 10.906
Solomon 10.994
Lehninger 10.965
Nozaki 10.73
DTASelect 10.57
Thurlkill 10.76
EMBOSS 11.169
Sillero 10.789
Patrickios 10.847
IPC_peptide 10.994
IPC2_peptide 9.355
IPC2.peptide.svr19 8.63
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2770
0
2770
702152
30
1291
253.5
28.43
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.67 ± 0.042
0.679 ± 0.017
4.901 ± 0.039
6.828 ± 0.061
4.684 ± 0.04
7.053 ± 0.048
1.406 ± 0.018
7.675 ± 0.054
7.001 ± 0.065
9.872 ± 0.059
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.814 ± 0.021
4.252 ± 0.038
4.029 ± 0.033
2.155 ± 0.027
4.635 ± 0.047
7.895 ± 0.055
4.897 ± 0.037
8.417 ± 0.045
0.982 ± 0.015
4.157 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here