Hypocrea jecorina (strain QM6a) (Trichoderma reesei)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Hypocreomycetidae; Hypocreales; Hypocreaceae; Trichoderma; Trichoderma reesei

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9114 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G0RDE9|G0RDE9_HYPJQ Predicted protein OS=Hypocrea jecorina (strain QM6a) OX=431241 GN=TRIREDRAFT_75713 PE=3 SV=1
MM1 pKa = 7.19QFPTAALALLSFTFTASAADD21 pKa = 3.77EE22 pKa = 4.24QLCFPAPGQTNNVPQSITDD41 pKa = 3.65LDD43 pKa = 4.03GQIKK47 pKa = 9.97IDD49 pKa = 3.64WATKK53 pKa = 9.48LCAQIDD59 pKa = 4.58YY60 pKa = 9.06STVDD64 pKa = 3.31AQSFTTDD71 pKa = 2.6IADD74 pKa = 3.56GVKK77 pKa = 9.4ATEE80 pKa = 3.84NGKK83 pKa = 8.06TYY85 pKa = 10.82GLNLVTVAVPNEE97 pKa = 4.06QKK99 pKa = 11.05CIDD102 pKa = 3.8NAAATLGADD111 pKa = 3.54VCPSGGAFINLDD123 pKa = 3.63NNEE126 pKa = 4.47EE127 pKa = 3.99EE128 pKa = 4.32WFSIVALDD136 pKa = 3.48

Molecular weight: 14.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G0R8L7|G0R8L7_HYPJQ Predicted protein OS=Hypocrea jecorina (strain QM6a) OX=431241 GN=TRIREDRAFT_102906 PE=4 SV=1
MM1 pKa = 7.76ASHH4 pKa = 6.06KK5 pKa = 8.34TFRR8 pKa = 11.84TKK10 pKa = 10.46QKK12 pKa = 9.86LAKK15 pKa = 9.55AQKK18 pKa = 8.93QNRR21 pKa = 11.84PVPQWIRR28 pKa = 11.84LRR30 pKa = 11.84TGNTIRR36 pKa = 11.84YY37 pKa = 5.79NAKK40 pKa = 8.89RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.14WRR45 pKa = 11.84KK46 pKa = 7.41TRR48 pKa = 11.84LGII51 pKa = 4.46

Molecular weight: 6.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9112

2

9114

4490027

48

20873

492.7

54.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.199 ± 0.026

1.212 ± 0.009

5.83 ± 0.019

6.17 ± 0.028

3.68 ± 0.016

6.89 ± 0.03

2.387 ± 0.011

4.76 ± 0.02

4.828 ± 0.022

8.981 ± 0.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.201 ± 0.009

3.484 ± 0.013

6.033 ± 0.029

4.041 ± 0.021

6.194 ± 0.022

8.089 ± 0.029

5.681 ± 0.017

6.163 ± 0.023

1.463 ± 0.008

2.715 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski