Prosthecomicrobium hirschii

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Hyphomicrobiaceae; Prosthecomicrobium

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5501 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0P6W3F8|A0A0P6W3F8_9RHIZ Sulfite dehydrogenase OS=Prosthecomicrobium hirschii OX=665126 GN=ABB55_17035 PE=4 SV=1
MM1 pKa = 7.28SLSGAINSAVSALRR15 pKa = 11.84AQSQALAVTSDD26 pKa = 3.57NIANAEE32 pKa = 4.16TYY34 pKa = 10.21GYY36 pKa = 7.76KK37 pKa = 9.91TSASFFEE44 pKa = 4.48SLVTSSGSTVTGSYY58 pKa = 10.31SAGGVQAATRR68 pKa = 11.84SNVTSSGLLEE78 pKa = 4.22STSNSTDD85 pKa = 3.43LAIDD89 pKa = 3.64GQGFFVVSAGDD100 pKa = 3.79GTDD103 pKa = 2.89TLYY106 pKa = 9.68YY107 pKa = 8.78TRR109 pKa = 11.84NGAFTVNTDD118 pKa = 4.28GEE120 pKa = 4.61LTNNGYY126 pKa = 7.74TLLGWPTDD134 pKa = 3.36ADD136 pKa = 3.84GTVTGGTNTEE146 pKa = 4.04NLTPIDD152 pKa = 3.74IDD154 pKa = 4.42SIASSASATTEE165 pKa = 3.65ASVVANLPANAEE177 pKa = 4.17TGDD180 pKa = 4.08TFTSTMDD187 pKa = 3.86LYY189 pKa = 11.42DD190 pKa = 3.76SLGTPAQSTMTWTKK204 pKa = 9.81TGEE207 pKa = 4.41NTWSLAFSDD216 pKa = 4.16PTASGSDD223 pKa = 3.13SATAIGTVSSSAITVTFNGDD243 pKa = 2.79GTLASTSPSPPTLTVSGWTSGAADD267 pKa = 3.47SSITLDD273 pKa = 3.58LGTAGSANGLSQYY286 pKa = 9.66TSSASTLTVSPTISQDD302 pKa = 2.82GKK304 pKa = 10.11PYY306 pKa = 9.43GTLSSIAVADD316 pKa = 4.55DD317 pKa = 3.58GTVNATYY324 pKa = 11.15SNGQTRR330 pKa = 11.84SIFKK334 pKa = 10.43IPVATFTNANGLEE347 pKa = 4.08AMSGAVYY354 pKa = 10.29RR355 pKa = 11.84VTDD358 pKa = 3.67EE359 pKa = 4.56SGASTLHH366 pKa = 6.64LAGADD371 pKa = 3.58GAGTVQGSTLEE382 pKa = 4.22ASTTDD387 pKa = 3.23TNEE390 pKa = 3.67QFSNMIAAQQAYY402 pKa = 9.24SAASKK407 pKa = 11.04VMSTANDD414 pKa = 3.41MFQTLLQVVSS424 pKa = 3.92

Molecular weight: 42.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0P6W460|A0A0P6W460_9RHIZ Peptidase C51 domain-containing protein OS=Prosthecomicrobium hirschii OX=665126 GN=ABB55_14150 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84LVIARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.98RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5501

0

5501

1765156

35

2688

320.9

34.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.196 ± 0.058

0.762 ± 0.01

5.755 ± 0.03

5.322 ± 0.035

3.589 ± 0.021

9.137 ± 0.043

1.874 ± 0.016

4.924 ± 0.023

2.767 ± 0.028

10.054 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.296 ± 0.014

2.099 ± 0.02

5.625 ± 0.03

2.572 ± 0.022

7.813 ± 0.042

4.75 ± 0.022

5.308 ± 0.033

7.829 ± 0.024

1.314 ± 0.012

2.013 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski