Amino acid dipepetide frequency for Prosthecomicrobium hirschii

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
23.509AlaAla: 23.509 ± 0.214
1.098AlaCys: 1.098 ± 0.026
8.05AlaAsp: 8.05 ± 0.078
8.557AlaGlu: 8.557 ± 0.081
4.853AlaPhe: 4.853 ± 0.062
13.533AlaGly: 13.533 ± 0.117
2.288AlaHis: 2.288 ± 0.04
6.793AlaIle: 6.793 ± 0.067
3.587AlaLys: 3.587 ± 0.062
14.45AlaLeu: 14.45 ± 0.12
3.566AlaMet: 3.566 ± 0.048
2.44AlaAsn: 2.44 ± 0.039
6.746AlaPro: 6.746 ± 0.084
3.232AlaGln: 3.232 ± 0.047
10.873AlaArg: 10.873 ± 0.102
6.188AlaSer: 6.188 ± 0.056
6.948AlaThr: 6.948 ± 0.073
10.861AlaVal: 10.861 ± 0.1
1.771AlaTrp: 1.771 ± 0.035
2.611AlaTyr: 2.611 ± 0.036
0.0AlaXaa: 0.0 ± 0.0
Cys
0.884CysAla: 0.884 ± 0.025
0.089CysCys: 0.089 ± 0.007
0.467CysAsp: 0.467 ± 0.017
0.348CysGlu: 0.348 ± 0.016
0.291CysPhe: 0.291 ± 0.014
0.916CysGly: 0.916 ± 0.027
0.217CysHis: 0.217 ± 0.012
0.288CysIle: 0.288 ± 0.014
0.138CysLys: 0.138 ± 0.01
0.785CysLeu: 0.785 ± 0.024
0.143CysMet: 0.143 ± 0.01
0.181CysAsn: 0.181 ± 0.01
0.406CysPro: 0.406 ± 0.016
0.17CysGln: 0.17 ± 0.01
0.764CysArg: 0.764 ± 0.024
0.363CysSer: 0.363 ± 0.016
0.356CysThr: 0.356 ± 0.015
0.546CysVal: 0.546 ± 0.017
0.105CysTrp: 0.105 ± 0.008
0.165CysTyr: 0.165 ± 0.009
0.0CysXaa: 0.0 ± 0.0
Asp
7.214AspAla: 7.214 ± 0.074
0.483AspCys: 0.483 ± 0.019
3.062AspAsp: 3.062 ± 0.056
3.159AspGlu: 3.159 ± 0.042
2.164AspPhe: 2.164 ± 0.036
5.784AspGly: 5.784 ± 0.064
1.181AspHis: 1.181 ± 0.034
2.667AspIle: 2.667 ± 0.044
1.475AspLys: 1.475 ± 0.037
6.594AspLeu: 6.594 ± 0.069
1.25AspMet: 1.25 ± 0.027
1.003AspAsn: 1.003 ± 0.027
4.174AspPro: 4.174 ± 0.052
1.548AspGln: 1.548 ± 0.031
5.557AspArg: 5.557 ± 0.065
1.788AspSer: 1.788 ± 0.042
2.337AspThr: 2.337 ± 0.056
3.797AspVal: 3.797 ± 0.05
0.973AspTrp: 0.973 ± 0.027
1.344AspTyr: 1.344 ± 0.031
0.0AspXaa: 0.0 ± 0.0
Glu
8.762GluAla: 8.762 ± 0.084
0.323GluCys: 0.323 ± 0.014
2.5GluAsp: 2.5 ± 0.041
2.474GluGlu: 2.474 ± 0.047
1.534GluPhe: 1.534 ± 0.033
3.966GluGly: 3.966 ± 0.044
0.977GluHis: 0.977 ± 0.024
3.443GluIle: 3.443 ± 0.047
1.773GluLys: 1.773 ± 0.041
4.5GluLeu: 4.5 ± 0.056
1.26GluMet: 1.26 ± 0.025
1.098GluAsn: 1.098 ± 0.029
3.056GluPro: 3.056 ± 0.048
1.523GluGln: 1.523 ± 0.033
5.16GluArg: 5.16 ± 0.062
1.932GluSer: 1.932 ± 0.035
3.827GluThr: 3.827 ± 0.051
3.692GluVal: 3.692 ± 0.051
0.619GluTrp: 0.619 ± 0.019
0.799GluTyr: 0.799 ± 0.023
0.0GluXaa: 0.0 ± 0.0
Phe
5.034PheAla: 5.034 ± 0.055
0.338PheCys: 0.338 ± 0.014
2.616PheAsp: 2.616 ± 0.041
2.098PheGlu: 2.098 ± 0.037
1.254PhePhe: 1.254 ± 0.029
3.909PheGly: 3.909 ± 0.053
0.706PheHis: 0.706 ± 0.019
1.233PheIle: 1.233 ± 0.026
0.893PheLys: 0.893 ± 0.026
3.295PheLeu: 3.295 ± 0.048
0.776PheMet: 0.776 ± 0.021
0.841PheAsn: 0.841 ± 0.02
1.536PhePro: 1.536 ± 0.034
0.879PheGln: 0.879 ± 0.025
2.536PheArg: 2.536 ± 0.037
1.755PheSer: 1.755 ± 0.035
1.804PheThr: 1.804 ± 0.031
3.109PheVal: 3.109 ± 0.046
0.501PheTrp: 0.501 ± 0.018
0.781PheTyr: 0.781 ± 0.025
0.0PheXaa: 0.0 ± 0.0
Gly
10.859GlyAla: 10.859 ± 0.095
0.815GlyCys: 0.815 ± 0.025
4.345GlyAsp: 4.345 ± 0.058
4.466GlyGlu: 4.466 ± 0.058
3.822GlyPhe: 3.822 ± 0.044
8.17GlyGly: 8.17 ± 0.112
1.992GlyHis: 1.992 ± 0.036
4.708GlyIle: 4.708 ± 0.058
2.704GlyLys: 2.704 ± 0.049
10.301GlyLeu: 10.301 ± 0.099
2.204GlyMet: 2.204 ± 0.034
1.878GlyAsn: 1.878 ± 0.074
4.648GlyPro: 4.648 ± 0.056
2.754GlyGln: 2.754 ± 0.068
8.092GlyArg: 8.092 ± 0.072
4.746GlySer: 4.746 ± 0.057
5.137GlyThr: 5.137 ± 0.077
6.016GlyVal: 6.016 ± 0.067
1.472GlyTrp: 1.472 ± 0.03
2.239GlyTyr: 2.239 ± 0.038
0.0GlyXaa: 0.0 ± 0.0
His
2.367HisAla: 2.367 ± 0.04
0.194HisCys: 0.194 ± 0.011
1.143HisAsp: 1.143 ± 0.03
0.881HisGlu: 0.881 ± 0.022
0.775HisPhe: 0.775 ± 0.023
1.924HisGly: 1.924 ± 0.042
0.523HisHis: 0.523 ± 0.019
0.748HisIle: 0.748 ± 0.021
0.361HisLys: 0.361 ± 0.015
1.974HisLeu: 1.974 ± 0.04
0.451HisMet: 0.451 ± 0.017
0.354HisAsn: 0.354 ± 0.013
1.357HisPro: 1.357 ± 0.029
0.498HisGln: 0.498 ± 0.017
1.442HisArg: 1.442 ± 0.029
0.717HisSer: 0.717 ± 0.025
0.717HisThr: 0.717 ± 0.022
1.523HisVal: 1.523 ± 0.034
0.296HisTrp: 0.296 ± 0.014
0.49HisTyr: 0.49 ± 0.018
0.0HisXaa: 0.0 ± 0.0
Ile
7.764IleAla: 7.764 ± 0.074
0.384IleCys: 0.384 ± 0.016
3.549IleAsp: 3.549 ± 0.047
3.49IleGlu: 3.49 ± 0.046
1.39IlePhe: 1.39 ± 0.028
5.292IleGly: 5.292 ± 0.06
0.836IleHis: 0.836 ± 0.022
1.548IleIle: 1.548 ± 0.033
1.138IleLys: 1.138 ± 0.029
4.737IleLeu: 4.737 ± 0.058
0.873IleMet: 0.873 ± 0.022
1.052IleAsn: 1.052 ± 0.03
2.332IlePro: 2.332 ± 0.038
1.037IleGln: 1.037 ± 0.025
3.639IleArg: 3.639 ± 0.046
2.06IleSer: 2.06 ± 0.039
2.215IleThr: 2.215 ± 0.034
4.439IleVal: 4.439 ± 0.057
0.519IleTrp: 0.519 ± 0.016
0.952IleTyr: 0.952 ± 0.021
0.0IleXaa: 0.0 ± 0.0
Lys
4.019LysAla: 4.019 ± 0.059
0.135LysCys: 0.135 ± 0.008
1.476LysAsp: 1.476 ± 0.035
1.118LysGlu: 1.118 ± 0.028
0.809LysPhe: 0.809 ± 0.022
2.303LysGly: 2.303 ± 0.041
0.401LysHis: 0.401 ± 0.016
1.396LysIle: 1.396 ± 0.031
0.965LysLys: 0.965 ± 0.034
2.643LysLeu: 2.643 ± 0.041
0.612LysMet: 0.612 ± 0.019
0.653LysAsn: 0.653 ± 0.023
1.975LysPro: 1.975 ± 0.039
0.702LysGln: 0.702 ± 0.022
2.015LysArg: 2.015 ± 0.036
1.643LysSer: 1.643 ± 0.033
1.741LysThr: 1.741 ± 0.033
2.235LysVal: 2.235 ± 0.05
0.299LysTrp: 0.299 ± 0.015
0.522LysTyr: 0.522 ± 0.018
0.0LysXaa: 0.0 ± 0.0
Leu
15.966LeuAla: 15.966 ± 0.145
0.787LeuCys: 0.787 ± 0.02
6.6LeuAsp: 6.6 ± 0.075
4.844LeuGlu: 4.844 ± 0.054
3.577LeuPhe: 3.577 ± 0.062
8.939LeuGly: 8.939 ± 0.085
1.641LeuHis: 1.641 ± 0.034
4.987LeuIle: 4.987 ± 0.062
3.392LeuLys: 3.392 ± 0.049
8.586LeuLeu: 8.586 ± 0.093
2.204LeuMet: 2.204 ± 0.032
2.092LeuAsn: 2.092 ± 0.037
5.669LeuPro: 5.669 ± 0.069
2.349LeuGln: 2.349 ± 0.039
6.584LeuArg: 6.584 ± 0.075
5.445LeuSer: 5.445 ± 0.062
5.427LeuThr: 5.427 ± 0.06
8.438LeuVal: 8.438 ± 0.085
1.139LeuTrp: 1.139 ± 0.026
1.868LeuTyr: 1.868 ± 0.031
0.0LeuXaa: 0.0 ± 0.0
Met
3.22MetAla: 3.22 ± 0.046
0.12MetCys: 0.12 ± 0.008
0.998MetAsp: 0.998 ± 0.027
0.9MetGlu: 0.9 ± 0.022
0.593MetPhe: 0.593 ± 0.018
1.628MetGly: 1.628 ± 0.033
0.377MetHis: 0.377 ± 0.014
1.271MetIle: 1.271 ± 0.027
0.809MetLys: 0.809 ± 0.022
2.266MetLeu: 2.266 ± 0.036
0.575MetMet: 0.575 ± 0.02
0.612MetAsn: 0.612 ± 0.018
1.552MetPro: 1.552 ± 0.032
0.67MetGln: 0.67 ± 0.02
1.795MetArg: 1.795 ± 0.031
1.442MetSer: 1.442 ± 0.027
1.858MetThr: 1.858 ± 0.034
1.851MetVal: 1.851 ± 0.032
0.168MetTrp: 0.168 ± 0.01
0.256MetTyr: 0.256 ± 0.012
0.0MetXaa: 0.0 ± 0.0
Asn
2.711AsnAla: 2.711 ± 0.039
0.215AsnCys: 0.215 ± 0.011
1.085AsnAsp: 1.085 ± 0.042
0.928AsnGlu: 0.928 ± 0.026
0.764AsnPhe: 0.764 ± 0.021
2.013AsnGly: 2.013 ± 0.042
0.386AsnHis: 0.386 ± 0.015
0.949AsnIle: 0.949 ± 0.023
0.554AsnLys: 0.554 ± 0.018
2.098AsnLeu: 2.098 ± 0.036
0.468AsnMet: 0.468 ± 0.015
0.505AsnAsn: 0.505 ± 0.018
1.57AsnPro: 1.57 ± 0.031
0.616AsnGln: 0.616 ± 0.021
1.737AsnArg: 1.737 ± 0.035
0.908AsnSer: 0.908 ± 0.029
1.019AsnThr: 1.019 ± 0.029
1.607AsnVal: 1.607 ± 0.037
0.328AsnTrp: 0.328 ± 0.015
0.53AsnTyr: 0.53 ± 0.017
0.0AsnXaa: 0.0 ± 0.0
Pro
8.378ProAla: 8.378 ± 0.093
0.309ProCys: 0.309 ± 0.012
4.117ProAsp: 4.117 ± 0.058
3.641ProGlu: 3.641 ± 0.048
2.034ProPhe: 2.034 ± 0.037
5.249ProGly: 5.249 ± 0.061
1.067ProHis: 1.067 ± 0.025
2.542ProIle: 2.542 ± 0.043
1.647ProLys: 1.647 ± 0.034
4.752ProLeu: 4.752 ± 0.057
1.208ProMet: 1.208 ± 0.026
1.3ProAsn: 1.3 ± 0.027
3.127ProPro: 3.127 ± 0.06
1.336ProGln: 1.336 ± 0.024
3.308ProArg: 3.308 ± 0.049
2.795ProSer: 2.795 ± 0.044
2.725ProThr: 2.725 ± 0.046
4.86ProVal: 4.86 ± 0.059
0.72ProTrp: 0.72 ± 0.02
1.137ProTyr: 1.137 ± 0.026
0.0ProXaa: 0.0 ± 0.0
Gln
3.601GlnAla: 3.601 ± 0.04
0.157GlnCys: 0.157 ± 0.011
1.281GlnAsp: 1.281 ± 0.028
1.177GlnGlu: 1.177 ± 0.03
0.898GlnPhe: 0.898 ± 0.024
2.123GlnGly: 2.123 ± 0.075
0.471GlnHis: 0.471 ± 0.018
1.598GlnIle: 1.598 ± 0.039
0.797GlnLys: 0.797 ± 0.024
2.234GlnLeu: 2.234 ± 0.031
0.706GlnMet: 0.706 ± 0.02
0.656GlnAsn: 0.656 ± 0.022
1.471GlnPro: 1.471 ± 0.03
0.836GlnGln: 0.836 ± 0.03
1.995GlnArg: 1.995 ± 0.039
1.466GlnSer: 1.466 ± 0.03
1.471GlnThr: 1.471 ± 0.029
1.996GlnVal: 1.996 ± 0.034
0.309GlnTrp: 0.309 ± 0.012
0.476GlnTyr: 0.476 ± 0.018
0.0GlnXaa: 0.0 ± 0.0
Arg
9.411ArgAla: 9.411 ± 0.089
0.509ArgCys: 0.509 ± 0.022
4.424ArgAsp: 4.424 ± 0.053
3.959ArgGlu: 3.959 ± 0.053
3.233ArgPhe: 3.233 ± 0.042
5.447ArgGly: 5.447 ± 0.06
1.873ArgHis: 1.873 ± 0.035
4.756ArgIle: 4.756 ± 0.057
1.934ArgLys: 1.934 ± 0.036
9.489ArgLeu: 9.489 ± 0.097
1.888ArgMet: 1.888 ± 0.032
1.603ArgAsn: 1.603 ± 0.026
4.665ArgPro: 4.665 ± 0.059
2.364ArgGln: 2.364 ± 0.036
7.078ArgArg: 7.078 ± 0.075
3.844ArgSer: 3.844 ± 0.05
3.775ArgThr: 3.775 ± 0.053
5.261ArgVal: 5.261 ± 0.051
1.047ArgTrp: 1.047 ± 0.028
1.572ArgTyr: 1.572 ± 0.032
0.0ArgXaa: 0.0 ± 0.0
Ser
5.979SerAla: 5.979 ± 0.049
0.327SerCys: 0.327 ± 0.016
2.592SerAsp: 2.592 ± 0.045
2.256SerGlu: 2.256 ± 0.042
1.919SerPhe: 1.919 ± 0.031
5.271SerGly: 5.271 ± 0.065
0.898SerHis: 0.898 ± 0.024
2.437SerIle: 2.437 ± 0.039
1.25SerLys: 1.25 ± 0.028
4.956SerLeu: 4.956 ± 0.052
1.146SerMet: 1.146 ± 0.026
1.047SerAsn: 1.047 ± 0.024
2.638SerPro: 2.638 ± 0.036
1.209SerGln: 1.209 ± 0.027
3.388SerArg: 3.388 ± 0.049
2.281SerSer: 2.281 ± 0.044
2.4SerThr: 2.4 ± 0.041
3.795SerVal: 3.795 ± 0.051
0.674SerTrp: 0.674 ± 0.022
1.036SerTyr: 1.036 ± 0.023
0.0SerXaa: 0.0 ± 0.0
Thr
7.163ThrAla: 7.163 ± 0.08
0.395ThrCys: 0.395 ± 0.015
2.765ThrAsp: 2.765 ± 0.039
2.629ThrGlu: 2.629 ± 0.044
1.881ThrPhe: 1.881 ± 0.034
5.647ThrGly: 5.647 ± 0.085
0.895ThrHis: 0.895 ± 0.026
2.801ThrIle: 2.801 ± 0.04
1.186ThrLys: 1.186 ± 0.028
5.669ThrLeu: 5.669 ± 0.078
1.087ThrMet: 1.087 ± 0.025
1.07ThrAsn: 1.07 ± 0.03
3.267ThrPro: 3.267 ± 0.048
1.105ThrGln: 1.105 ± 0.028
3.671ThrArg: 3.671 ± 0.045
2.356ThrSer: 2.356 ± 0.039
2.794ThrThr: 2.794 ± 0.041
4.995ThrVal: 4.995 ± 0.059
0.667ThrTrp: 0.667 ± 0.019
1.04ThrTyr: 1.04 ± 0.029
0.0ThrXaa: 0.0 ± 0.0
Val
11.28ValAla: 11.28 ± 0.094
0.598ValCys: 0.598 ± 0.018
4.516ValAsp: 4.516 ± 0.055
4.71ValGlu: 4.71 ± 0.053
2.809ValPhe: 2.809 ± 0.045
6.219ValGly: 6.219 ± 0.075
1.321ValHis: 1.321 ± 0.03
3.752ValIle: 3.752 ± 0.044
2.135ValLys: 2.135 ± 0.044
7.594ValLeu: 7.594 ± 0.08
1.828ValMet: 1.828 ± 0.033
1.769ValAsn: 1.769 ± 0.033
4.313ValPro: 4.313 ± 0.06
1.833ValGln: 1.833 ± 0.031
5.361ValArg: 5.361 ± 0.058
4.043ValSer: 4.043 ± 0.049
4.759ValThr: 4.759 ± 0.056
6.949ValVal: 6.949 ± 0.079
0.989ValTrp: 0.989 ± 0.024
1.518ValTyr: 1.518 ± 0.031
0.0ValXaa: 0.0 ± 0.0
Trp
1.333TrpAla: 1.333 ± 0.027
0.134TrpCys: 0.134 ± 0.009
0.641TrpAsp: 0.641 ± 0.022
0.486TrpGlu: 0.486 ± 0.016
0.533TrpPhe: 0.533 ± 0.02
0.955TrpGly: 0.955 ± 0.026
0.306TrpHis: 0.306 ± 0.014
0.655TrpIle: 0.655 ± 0.018
0.407TrpLys: 0.407 ± 0.017
1.541TrpLeu: 1.541 ± 0.037
0.325TrpMet: 0.325 ± 0.013
0.407TrpAsn: 0.407 ± 0.016
0.701TrpPro: 0.701 ± 0.022
0.454TrpGln: 0.454 ± 0.015
1.261TrpArg: 1.261 ± 0.029
0.815TrpSer: 0.815 ± 0.024
0.857TrpThr: 0.857 ± 0.021
0.825TrpVal: 0.825 ± 0.022
0.223TrpTrp: 0.223 ± 0.011
0.28TrpTyr: 0.28 ± 0.015
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.501TyrAla: 2.501 ± 0.037
0.209TyrCys: 0.209 ± 0.01
1.325TyrAsp: 1.325 ± 0.024
1.098TyrGlu: 1.098 ± 0.026
0.757TyrPhe: 0.757 ± 0.023
2.08TyrGly: 2.08 ± 0.036
0.381TyrHis: 0.381 ± 0.014
0.672TyrIle: 0.672 ± 0.021
0.521TyrLys: 0.521 ± 0.02
2.071TyrLeu: 2.071 ± 0.039
0.389TyrMet: 0.389 ± 0.017
0.432TyrAsn: 0.432 ± 0.018
1.048TyrPro: 1.048 ± 0.025
0.605TyrGln: 0.605 ± 0.021
1.876TyrArg: 1.876 ± 0.035
0.913TyrSer: 0.913 ± 0.025
0.914TyrThr: 0.914 ± 0.026
1.499TyrVal: 1.499 ± 0.03
0.324TyrTrp: 0.324 ± 0.014
0.515TyrTyr: 0.515 ± 0.021
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5501 proteins (1765156 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski