Candidatus Jettenia caeni
Average proteome isoelectric point is 7.0
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3545 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|I3IM23|I3IM23_9BACT Uncharacterized protein OS=Candidatus Jettenia caeni OX=247490 GN=KSU1_C1172 PE=4 SV=1
MM1 pKa = 7.53 LPKK4 pKa = 10.09 ILPSVLTMPFAVVLASLLFIVNLATAALPSYY35 pKa = 10.48 DD36 pKa = 3.62 LQIYY40 pKa = 8.82 KK41 pKa = 10.35 QGTWYY46 pKa = 9.56 TGIAMEE52 pKa = 6.39 DD53 pKa = 3.3 IDD55 pKa = 4.93 NDD57 pKa = 3.76 GGLEE61 pKa = 3.93 IIIGNRR67 pKa = 11.84 NTSSAEE73 pKa = 3.1 IWKK76 pKa = 10.35 YY77 pKa = 10.64 NVSTQTLNQTASISFPYY94 pKa = 9.66 HH95 pKa = 5.21 IHH97 pKa = 7.63 DD98 pKa = 3.65 IVAVDD103 pKa = 3.88 VDD105 pKa = 3.7 HH106 pKa = 7.67 DD107 pKa = 4.15 GQKK110 pKa = 10.68 EE111 pKa = 4.08 IIVGIRR117 pKa = 11.84 FEE119 pKa = 4.42 GLYY122 pKa = 8.88 MARR125 pKa = 11.84 FSGSAWNVTQIADD138 pKa = 4.11 GYY140 pKa = 7.5 TWQVLAADD148 pKa = 4.71 FNGDD152 pKa = 3.35 GHH154 pKa = 8.34 VDD156 pKa = 3.03 ICQGLDD162 pKa = 2.85 HH163 pKa = 7.13 AYY165 pKa = 9.78 IRR167 pKa = 11.84 IFYY170 pKa = 9.57 GNGVGGFTLGSSPPLEE186 pKa = 4.03 RR187 pKa = 11.84 QLGNGWGMNAIDD199 pKa = 4.7 LNSDD203 pKa = 2.97 GRR205 pKa = 11.84 LDD207 pKa = 4.66 LIGVVPQWPDD217 pKa = 3.47 GIGTGYY223 pKa = 10.48 FLRR226 pKa = 11.84 AHH228 pKa = 6.99 LNLDD232 pKa = 3.27 AGGQVTWNSVGPTAPLSYY250 pKa = 10.47 DD251 pKa = 3.28 QNRR254 pKa = 11.84 AQAFLSNSAADD265 pKa = 3.43 IDD267 pKa = 4.29 GNGYY271 pKa = 9.38 IDD273 pKa = 3.47 QAVFFANGRR282 pKa = 11.84 VSIYY286 pKa = 9.53 MGSSSGGQLVWTEE299 pKa = 3.79 QQIAQLPTTDD309 pKa = 6.49 FIDD312 pKa = 3.58 QPSIGISDD320 pKa = 3.77 VNNDD324 pKa = 2.87 GFLDD328 pKa = 3.53 VHH330 pKa = 7.69 VDD332 pKa = 3.55 GGADD336 pKa = 3.54 FNGMVVFLGDD346 pKa = 3.53 GTGMYY351 pKa = 8.09 QQKK354 pKa = 8.18 TLILDD359 pKa = 3.45 HH360 pKa = 7.04 GIGGFLNSTRR370 pKa = 11.84 FGDD373 pKa = 3.72 INGDD377 pKa = 3.74 GYY379 pKa = 9.69 TDD381 pKa = 3.27 ICAVRR386 pKa = 11.84 FMGNSYY392 pKa = 11.27 AGFDD396 pKa = 3.67 VLYY399 pKa = 7.85 QTPPSNLPPVANAGTDD415 pKa = 3.45 QSVHH419 pKa = 5.54 VGNTVMLNGSGSSDD433 pKa = 3.53 PNGDD437 pKa = 3.71 LLTYY441 pKa = 10.43 NWTFISKK448 pKa = 9.91 PGGSSATLSDD458 pKa = 3.71 PTLVNPTFTVDD469 pKa = 2.85 KK470 pKa = 10.91 AGTYY474 pKa = 9.22 KK475 pKa = 10.78 VKK477 pKa = 10.79 LVVNDD482 pKa = 3.81 GTVDD486 pKa = 3.67 SVADD490 pKa = 3.64 TVLISTINVKK500 pKa = 9.99 PVANAGDD507 pKa = 3.9 DD508 pKa = 3.37 QSVYY512 pKa = 10.76 VGSLVTLDD520 pKa = 4.25 GSGSSDD526 pKa = 3.41 PDD528 pKa = 3.54 GDD530 pKa = 3.57 QVTYY534 pKa = 10.92 NWAFQSKK541 pKa = 8.43 PQNSSATLNNPTSASPTFTVDD562 pKa = 2.64 KK563 pKa = 10.87 AGTYY567 pKa = 7.84 VVKK570 pKa = 11.02 LIVNDD575 pKa = 4.01 GALNSDD581 pKa = 4.18 PDD583 pKa = 3.84 TVTISNINVAPVADD597 pKa = 4.1 AGMDD601 pKa = 3.49 QAITTIGTTVQLDD614 pKa = 3.66 GTQSYY619 pKa = 11.1 DD620 pKa = 3.42 PDD622 pKa = 4.69 GDD624 pKa = 4.58 PITYY628 pKa = 9.21 QWAFTSIPDD637 pKa = 3.52 GSTASLADD645 pKa = 4.14 ADD647 pKa = 4.32 TATPTFTADD656 pKa = 2.98 VHH658 pKa = 6.98 GEE660 pKa = 4.13 YY661 pKa = 10.55 VVQLVVKK668 pKa = 9.13 DD669 pKa = 3.93 TSLLSAQDD677 pKa = 3.35 AVVISFTNIKK687 pKa = 9.5 PVANAGTSQSAVVGDD702 pKa = 3.77 TVTINGSGSTDD713 pKa = 3.08 GNGDD717 pKa = 3.34 NLTYY721 pKa = 10.09 QWSFSSVPDD730 pKa = 3.8 GSNAEE735 pKa = 4.14 IAGPTAITTTFVPDD749 pKa = 3.21 KK750 pKa = 10.54 AGTYY754 pKa = 9.32 VVQLVVNDD762 pKa = 5.58 GIVDD766 pKa = 4.0 SDD768 pKa = 3.93 PSTVQVQVIDD778 pKa = 3.95 EE779 pKa = 4.05 QTAAIQAIQDD789 pKa = 3.99 CEE791 pKa = 3.95 QVIASLNSNVFKK803 pKa = 10.59 NANMQNTLINKK814 pKa = 9.18 LNAVIANIEE823 pKa = 3.97 AGNYY827 pKa = 8.86 EE828 pKa = 4.28 DD829 pKa = 6.22 ALGQLRR835 pKa = 11.84 NDD837 pKa = 3.58 VLGKK841 pKa = 9.1 TDD843 pKa = 3.08 GCADD847 pKa = 3.6 SGSPDD852 pKa = 3.33 KK853 pKa = 11.39 NDD855 pKa = 3.82 WIKK858 pKa = 11.41 DD859 pKa = 3.5 CAAQGSVYY867 pKa = 10.43 PCIQHH872 pKa = 6.86 AIVVVEE878 pKa = 3.94 GLL880 pKa = 3.55
Molecular weight: 92.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.678
IPC2_protein 3.795
IPC_protein 3.872
Toseland 3.617
ProMoST 4.05
Dawson 3.884
Bjellqvist 4.037
Wikipedia 3.859
Rodwell 3.681
Grimsley 3.516
Solomon 3.884
Lehninger 3.834
Nozaki 3.986
DTASelect 4.329
Thurlkill 3.681
EMBOSS 3.859
Sillero 3.999
Patrickios 1.558
IPC_peptide 3.872
IPC2_peptide 3.961
IPC2.peptide.svr19 3.869
Protein with the highest isoelectric point:
>tr|I3IH07|I3IH07_9BACT Gas vesicle structural protein OS=Candidatus Jettenia caeni OX=247490 GN=KSU1_B0145 PE=3 SV=1
MM1 pKa = 7.42 KK2 pKa = 10.72 VNIRR6 pKa = 11.84 KK7 pKa = 9.66 SSIKK11 pKa = 9.61 HH12 pKa = 5.21 KK13 pKa = 10.38 RR14 pKa = 11.84 MCGFRR19 pKa = 11.84 KK20 pKa = 9.96 RR21 pKa = 11.84 MRR23 pKa = 11.84 TKK25 pKa = 10.35 GGRR28 pKa = 11.84 AILKK32 pKa = 8.33 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 IGRR39 pKa = 11.84 RR40 pKa = 11.84 PLLDD44 pKa = 3.21 VV45 pKa = 4.01
Molecular weight: 5.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.348
IPC2_protein 10.745
IPC_protein 12.325
Toseland 12.486
ProMoST 12.983
Dawson 12.486
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.223
Grimsley 12.53
Solomon 12.983
Lehninger 12.881
Nozaki 12.486
DTASelect 12.486
Thurlkill 12.486
EMBOSS 12.983
Sillero 12.486
Patrickios 11.959
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.101
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3545
0
3545
1108176
37
3982
312.6
35.26
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.453 ± 0.032
1.299 ± 0.02
5.24 ± 0.039
6.824 ± 0.048
4.485 ± 0.033
6.765 ± 0.043
2.241 ± 0.021
8.569 ± 0.046
7.403 ± 0.049
9.294 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.378 ± 0.02
4.467 ± 0.028
3.958 ± 0.027
3.196 ± 0.023
4.873 ± 0.032
6.095 ± 0.039
5.424 ± 0.044
6.364 ± 0.036
1.073 ± 0.016
3.598 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here