Salmonella phage 9NA
Average proteome isoelectric point is 6.42
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 84 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A060D1I7|A0A060D1I7_9CAUD Uncharacterized protein OS=Salmonella phage 9NA OX=1113547 GN=9NA_015 PE=4 SV=1
MM1 pKa = 7.83 KK2 pKa = 9.23 ITKK5 pKa = 9.7 QEE7 pKa = 3.99 LKK9 pKa = 10.99 DD10 pKa = 4.7 FGACQDD16 pKa = 3.33 GFKK19 pKa = 10.75 RR20 pKa = 11.84 FVNQTNNTDD29 pKa = 3.39 EE30 pKa = 4.49 PVDD33 pKa = 3.9 VVSLIGGCNTVSDD46 pKa = 4.7 LLWLAGKK53 pKa = 7.87 TLAKK57 pKa = 10.11 EE58 pKa = 3.95 KK59 pKa = 10.2 IVRR62 pKa = 11.84 FACDD66 pKa = 3.1 CALLNIEE73 pKa = 5.18 KK74 pKa = 10.04 IRR76 pKa = 11.84 PYY78 pKa = 10.44 TNDD81 pKa = 2.94 YY82 pKa = 10.8 NLIVDD87 pKa = 4.77 FLNTPTADD95 pKa = 3.3 AARR98 pKa = 11.84 AADD101 pKa = 3.64 AAYY104 pKa = 10.05 AAARR108 pKa = 11.84 AADD111 pKa = 3.53 AAAYY115 pKa = 8.96 AAARR119 pKa = 11.84 AADD122 pKa = 3.52 AAAYY126 pKa = 8.89 AAYY129 pKa = 9.93 AAYY132 pKa = 9.91 AAAADD137 pKa = 3.83 AAYY140 pKa = 10.05 AAYY143 pKa = 9.92 AAYY146 pKa = 9.91 AAAADD151 pKa = 3.92 AADD154 pKa = 4.28 AADD157 pKa = 3.89 AAYY160 pKa = 10.65 ADD162 pKa = 4.26 AADD165 pKa = 4.37 AADD168 pKa = 4.13 AAAADD173 pKa = 3.96 AAYY176 pKa = 10.03 AAADD180 pKa = 3.55 AAYY183 pKa = 10.03 AAAADD188 pKa = 3.55 AAKK191 pKa = 10.34 INKK194 pKa = 9.35 LLVKK198 pKa = 10.29 LFSEE202 pKa = 4.76
Molecular weight: 20.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.049
IPC2_protein 4.215
IPC_protein 4.215
Toseland 3.973
ProMoST 4.342
Dawson 4.215
Bjellqvist 4.406
Wikipedia 4.177
Rodwell 4.037
Grimsley 3.884
Solomon 4.215
Lehninger 4.164
Nozaki 4.329
DTASelect 4.635
Thurlkill 4.037
EMBOSS 4.19
Sillero 4.329
Patrickios 2.778
IPC_peptide 4.202
IPC2_peptide 4.304
IPC2.peptide.svr19 4.256
Protein with the highest isoelectric point:
>tr|A0A060D562|A0A060D562_9CAUD Large terminase subunit OS=Salmonella phage 9NA OX=1113547 GN=9NA_011 PE=4 SV=1
MM1 pKa = 7.62 WNICHH6 pKa = 7.05 KK7 pKa = 10.16 SGRR10 pKa = 11.84 VLFTTGNKK18 pKa = 6.69 QTALNRR24 pKa = 11.84 AKK26 pKa = 10.82 YY27 pKa = 8.09 GWRR30 pKa = 11.84 VEE32 pKa = 4.15 KK33 pKa = 10.88 VNKK36 pKa = 9.52 EE37 pKa = 3.83 FQINEE42 pKa = 3.31 IWLSPRR48 pKa = 11.84 GYY50 pKa = 9.36 FYY52 pKa = 11.26 KK53 pKa = 10.9 VIDD56 pKa = 4.31 IEE58 pKa = 4.3 NKK60 pKa = 9.68 HH61 pKa = 6.86 AILKK65 pKa = 9.33 MGTHH69 pKa = 6.24 GLGRR73 pKa = 11.84 KK74 pKa = 8.65 IKK76 pKa = 9.95 RR77 pKa = 11.84 RR78 pKa = 11.84 KK79 pKa = 8.88 DD80 pKa = 3.25 TTKK83 pKa = 10.27 NWQLHH88 pKa = 4.38 TKK90 pKa = 9.88 VYY92 pKa = 11.22 NNGG95 pKa = 3.14
Molecular weight: 11.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.239
IPC2_protein 9.78
IPC_protein 9.955
Toseland 10.657
ProMoST 10.189
Dawson 10.76
Bjellqvist 10.365
Wikipedia 10.877
Rodwell 11.345
Grimsley 10.789
Solomon 10.789
Lehninger 10.774
Nozaki 10.628
DTASelect 10.365
Thurlkill 10.643
EMBOSS 11.038
Sillero 10.672
Patrickios 11.082
IPC_peptide 10.804
IPC2_peptide 8.975
IPC2.peptide.svr19 8.66
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
84
0
84
16269
40
971
193.7
21.61
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.882 ± 0.639
1.211 ± 0.148
6.233 ± 0.234
5.655 ± 0.201
3.823 ± 0.231
7.149 ± 0.264
1.813 ± 0.172
5.839 ± 0.215
5.723 ± 0.312
7.702 ± 0.235
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.606 ± 0.148
5.003 ± 0.249
4.407 ± 0.32
4.29 ± 0.583
5.249 ± 0.262
5.52 ± 0.225
6.485 ± 0.318
7.019 ± 0.292
1.678 ± 0.126
3.713 ± 0.194
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here