Amino acid dipepetide frequency for Salmonella phage 9NA

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.556AlaAla: 11.556 ± 2.486
0.861AlaCys: 0.861 ± 0.235
6.209AlaAsp: 6.209 ± 0.913
5.409AlaGlu: 5.409 ± 0.773
2.582AlaPhe: 2.582 ± 0.462
6.024AlaGly: 6.024 ± 0.679
1.475AlaHis: 1.475 ± 0.305
4.241AlaIle: 4.241 ± 0.489
4.119AlaLys: 4.119 ± 0.547
6.147AlaLeu: 6.147 ± 0.674
3.258AlaMet: 3.258 ± 0.503
4.119AlaAsn: 4.119 ± 0.561
4.364AlaPro: 4.364 ± 1.201
4.057AlaGln: 4.057 ± 0.844
3.873AlaArg: 3.873 ± 0.523
5.102AlaSer: 5.102 ± 0.672
5.286AlaThr: 5.286 ± 1.063
5.286AlaVal: 5.286 ± 0.519
1.168AlaTrp: 1.168 ± 0.352
3.688AlaTyr: 3.688 ± 0.803
0.0AlaXaa: 0.0 ± 0.0
Cys
0.922CysAla: 0.922 ± 0.248
0.184CysCys: 0.184 ± 0.13
0.984CysAsp: 0.984 ± 0.242
0.738CysGlu: 0.738 ± 0.205
0.492CysPhe: 0.492 ± 0.162
0.492CysGly: 0.492 ± 0.172
0.246CysHis: 0.246 ± 0.129
0.553CysIle: 0.553 ± 0.176
0.553CysLys: 0.553 ± 0.197
0.799CysLeu: 0.799 ± 0.196
0.246CysMet: 0.246 ± 0.123
0.738CysAsn: 0.738 ± 0.186
0.799CysPro: 0.799 ± 0.32
0.553CysGln: 0.553 ± 0.165
0.799CysArg: 0.799 ± 0.233
0.307CysSer: 0.307 ± 0.13
0.799CysThr: 0.799 ± 0.235
0.799CysVal: 0.799 ± 0.222
0.246CysTrp: 0.246 ± 0.122
0.861CysTyr: 0.861 ± 0.272
0.0CysXaa: 0.0 ± 0.0
Asp
6.024AspAla: 6.024 ± 0.956
0.738AspCys: 0.738 ± 0.233
4.856AspAsp: 4.856 ± 0.536
3.381AspGlu: 3.381 ± 0.397
2.889AspPhe: 2.889 ± 0.4
4.856AspGly: 4.856 ± 0.747
1.045AspHis: 1.045 ± 0.293
3.75AspIle: 3.75 ± 0.433
3.504AspLys: 3.504 ± 0.448
3.934AspLeu: 3.934 ± 0.445
1.537AspMet: 1.537 ± 0.258
2.52AspAsn: 2.52 ± 0.4
3.012AspPro: 3.012 ± 0.441
1.967AspGln: 1.967 ± 0.392
4.119AspArg: 4.119 ± 0.558
2.09AspSer: 2.09 ± 0.281
3.258AspThr: 3.258 ± 0.532
4.979AspVal: 4.979 ± 0.638
1.291AspTrp: 1.291 ± 0.309
2.582AspTyr: 2.582 ± 0.463
0.0AspXaa: 0.0 ± 0.0
Glu
4.364GluAla: 4.364 ± 0.603
0.799GluCys: 0.799 ± 0.189
2.336GluAsp: 2.336 ± 0.422
2.582GluGlu: 2.582 ± 0.415
2.336GluPhe: 2.336 ± 0.336
3.135GluGly: 3.135 ± 0.549
0.861GluHis: 0.861 ± 0.26
3.442GluIle: 3.442 ± 0.442
4.119GluLys: 4.119 ± 0.642
6.086GluLeu: 6.086 ± 0.651
1.291GluMet: 1.291 ± 0.362
2.582GluAsn: 2.582 ± 0.361
2.336GluPro: 2.336 ± 0.43
4.303GluGln: 4.303 ± 0.59
4.364GluArg: 4.364 ± 0.487
2.459GluSer: 2.459 ± 0.456
2.52GluThr: 2.52 ± 0.509
3.012GluVal: 3.012 ± 0.468
1.414GluTrp: 1.414 ± 0.261
2.213GluTyr: 2.213 ± 0.349
0.0GluXaa: 0.0 ± 0.0
Phe
2.705PheAla: 2.705 ± 0.452
0.43PheCys: 0.43 ± 0.157
2.397PheAsp: 2.397 ± 0.344
2.213PheGlu: 2.213 ± 0.382
1.414PhePhe: 1.414 ± 0.307
3.135PheGly: 3.135 ± 0.394
0.553PheHis: 0.553 ± 0.186
2.889PheIle: 2.889 ± 0.45
2.705PheLys: 2.705 ± 0.414
2.643PheLeu: 2.643 ± 0.429
0.799PheMet: 0.799 ± 0.216
1.475PheAsn: 1.475 ± 0.361
1.783PhePro: 1.783 ± 0.279
1.106PheGln: 1.106 ± 0.228
1.598PheArg: 1.598 ± 0.308
2.397PheSer: 2.397 ± 0.411
2.951PheThr: 2.951 ± 0.426
2.951PheVal: 2.951 ± 0.373
0.676PheTrp: 0.676 ± 0.199
1.414PheTyr: 1.414 ± 0.293
0.0PheXaa: 0.0 ± 0.0
Gly
5.594GlyAla: 5.594 ± 0.626
0.922GlyCys: 0.922 ± 0.294
4.18GlyAsp: 4.18 ± 0.501
4.795GlyGlu: 4.795 ± 0.609
3.442GlyPhe: 3.442 ± 0.538
4.672GlyGly: 4.672 ± 0.888
1.229GlyHis: 1.229 ± 0.29
4.241GlyIle: 4.241 ± 0.451
4.672GlyLys: 4.672 ± 0.471
5.102GlyLeu: 5.102 ± 0.555
1.967GlyMet: 1.967 ± 0.342
3.75GlyAsn: 3.75 ± 0.469
1.537GlyPro: 1.537 ± 0.242
2.705GlyGln: 2.705 ± 0.376
3.381GlyArg: 3.381 ± 0.531
4.795GlySer: 4.795 ± 0.575
4.733GlyThr: 4.733 ± 0.476
5.164GlyVal: 5.164 ± 0.535
1.291GlyTrp: 1.291 ± 0.284
3.319GlyTyr: 3.319 ± 0.438
0.0GlyXaa: 0.0 ± 0.0
His
0.922HisAla: 0.922 ± 0.261
0.492HisCys: 0.492 ± 0.17
1.229HisAsp: 1.229 ± 0.249
1.045HisGlu: 1.045 ± 0.265
0.492HisPhe: 0.492 ± 0.153
1.844HisGly: 1.844 ± 0.417
0.307HisHis: 0.307 ± 0.142
1.229HisIle: 1.229 ± 0.315
1.045HisLys: 1.045 ± 0.259
1.537HisLeu: 1.537 ± 0.276
0.307HisMet: 0.307 ± 0.164
0.799HisAsn: 0.799 ± 0.226
0.984HisPro: 0.984 ± 0.261
0.615HisGln: 0.615 ± 0.185
0.799HisArg: 0.799 ± 0.218
0.861HisSer: 0.861 ± 0.249
1.414HisThr: 1.414 ± 0.363
1.168HisVal: 1.168 ± 0.244
0.184HisTrp: 0.184 ± 0.094
0.861HisTyr: 0.861 ± 0.192
0.0HisXaa: 0.0 ± 0.0
Ile
4.856IleAla: 4.856 ± 0.548
0.922IleCys: 0.922 ± 0.274
5.286IleAsp: 5.286 ± 0.544
3.75IleGlu: 3.75 ± 0.523
1.967IlePhe: 1.967 ± 0.294
3.873IleGly: 3.873 ± 0.38
1.229IleHis: 1.229 ± 0.357
3.442IleIle: 3.442 ± 0.451
3.135IleLys: 3.135 ± 0.541
3.565IleLeu: 3.565 ± 0.448
1.537IleMet: 1.537 ± 0.268
3.381IleAsn: 3.381 ± 0.388
2.274IlePro: 2.274 ± 0.384
2.213IleGln: 2.213 ± 0.339
2.951IleArg: 2.951 ± 0.413
3.565IleSer: 3.565 ± 0.39
5.102IleThr: 5.102 ± 0.552
2.889IleVal: 2.889 ± 0.431
0.492IleTrp: 0.492 ± 0.182
1.967IleTyr: 1.967 ± 0.399
0.0IleXaa: 0.0 ± 0.0
Lys
5.348LysAla: 5.348 ± 0.705
0.492LysCys: 0.492 ± 0.197
3.196LysAsp: 3.196 ± 0.472
3.258LysGlu: 3.258 ± 0.436
2.397LysPhe: 2.397 ± 0.512
3.258LysGly: 3.258 ± 0.388
1.106LysHis: 1.106 ± 0.269
3.258LysIle: 3.258 ± 0.408
3.381LysLys: 3.381 ± 0.495
5.594LysLeu: 5.594 ± 0.698
1.783LysMet: 1.783 ± 0.305
2.09LysAsn: 2.09 ± 0.406
2.397LysPro: 2.397 ± 0.388
3.135LysGln: 3.135 ± 0.507
3.565LysArg: 3.565 ± 0.457
2.766LysSer: 2.766 ± 0.424
3.319LysThr: 3.319 ± 0.464
3.996LysVal: 3.996 ± 0.457
0.799LysTrp: 0.799 ± 0.252
2.09LysTyr: 2.09 ± 0.413
0.0LysXaa: 0.0 ± 0.0
Leu
6.393LeuAla: 6.393 ± 0.692
0.615LeuCys: 0.615 ± 0.176
4.057LeuAsp: 4.057 ± 0.394
4.672LeuGlu: 4.672 ± 0.592
2.951LeuPhe: 2.951 ± 0.432
3.873LeuGly: 3.873 ± 0.416
1.537LeuHis: 1.537 ± 0.305
4.364LeuIle: 4.364 ± 0.443
5.041LeuLys: 5.041 ± 0.595
5.655LeuLeu: 5.655 ± 0.621
2.213LeuMet: 2.213 ± 0.41
3.996LeuAsn: 3.996 ± 0.567
4.057LeuPro: 4.057 ± 0.475
3.258LeuGln: 3.258 ± 0.522
4.426LeuArg: 4.426 ± 0.637
5.471LeuSer: 5.471 ± 0.529
5.84LeuThr: 5.84 ± 0.533
4.487LeuVal: 4.487 ± 0.442
1.475LeuTrp: 1.475 ± 0.327
2.582LeuTyr: 2.582 ± 0.467
0.0LeuXaa: 0.0 ± 0.0
Met
2.459MetAla: 2.459 ± 0.408
0.369MetCys: 0.369 ± 0.145
0.984MetAsp: 0.984 ± 0.237
0.922MetGlu: 0.922 ± 0.24
0.984MetPhe: 0.984 ± 0.242
1.783MetGly: 1.783 ± 0.349
0.799MetHis: 0.799 ± 0.208
1.844MetIle: 1.844 ± 0.286
2.151MetLys: 2.151 ± 0.397
2.029MetLeu: 2.029 ± 0.301
0.369MetMet: 0.369 ± 0.167
1.352MetAsn: 1.352 ± 0.276
0.922MetPro: 0.922 ± 0.264
0.922MetGln: 0.922 ± 0.225
1.229MetArg: 1.229 ± 0.26
2.029MetSer: 2.029 ± 0.348
2.397MetThr: 2.397 ± 0.378
1.537MetVal: 1.537 ± 0.315
0.553MetTrp: 0.553 ± 0.178
0.43MetTyr: 0.43 ± 0.156
0.0MetXaa: 0.0 ± 0.0
Asn
4.364AsnAla: 4.364 ± 0.388
0.676AsnCys: 0.676 ± 0.174
2.643AsnAsp: 2.643 ± 0.357
2.766AsnGlu: 2.766 ± 0.395
1.721AsnPhe: 1.721 ± 0.348
4.549AsnGly: 4.549 ± 0.524
1.168AsnHis: 1.168 ± 0.282
3.196AsnIle: 3.196 ± 0.491
2.828AsnLys: 2.828 ± 0.438
3.381AsnLeu: 3.381 ± 0.674
1.168AsnMet: 1.168 ± 0.23
3.012AsnAsn: 3.012 ± 0.458
2.582AsnPro: 2.582 ± 0.462
2.336AsnGln: 2.336 ± 0.468
2.151AsnArg: 2.151 ± 0.338
2.889AsnSer: 2.889 ± 0.421
3.074AsnThr: 3.074 ± 0.416
3.196AsnVal: 3.196 ± 0.536
0.738AsnTrp: 0.738 ± 0.234
1.598AsnTyr: 1.598 ± 0.314
0.0AsnXaa: 0.0 ± 0.0
Pro
4.364ProAla: 4.364 ± 0.975
0.369ProCys: 0.369 ± 0.149
3.319ProAsp: 3.319 ± 0.541
2.52ProGlu: 2.52 ± 0.48
1.168ProPhe: 1.168 ± 0.259
4.241ProGly: 4.241 ± 0.47
1.229ProHis: 1.229 ± 0.271
1.906ProIle: 1.906 ± 0.3
1.844ProLys: 1.844 ± 0.308
2.582ProLeu: 2.582 ± 0.54
0.861ProMet: 0.861 ± 0.261
2.029ProAsn: 2.029 ± 0.357
1.66ProPro: 1.66 ± 0.257
0.676ProGln: 0.676 ± 0.174
2.151ProArg: 2.151 ± 0.353
2.029ProSer: 2.029 ± 0.387
2.643ProThr: 2.643 ± 0.377
5.409ProVal: 5.409 ± 0.984
1.106ProTrp: 1.106 ± 0.33
1.967ProTyr: 1.967 ± 0.345
0.0ProXaa: 0.0 ± 0.0
Gln
4.487GlnAla: 4.487 ± 0.884
0.615GlnCys: 0.615 ± 0.177
1.844GlnAsp: 1.844 ± 0.329
2.213GlnGlu: 2.213 ± 0.386
2.274GlnPhe: 2.274 ± 0.374
2.459GlnGly: 2.459 ± 0.385
0.676GlnHis: 0.676 ± 0.214
2.397GlnIle: 2.397 ± 0.482
2.09GlnLys: 2.09 ± 0.489
3.934GlnLeu: 3.934 ± 0.637
1.168GlnMet: 1.168 ± 0.266
1.414GlnAsn: 1.414 ± 0.359
1.66GlnPro: 1.66 ± 0.324
2.828GlnGln: 2.828 ± 0.865
2.951GlnArg: 2.951 ± 0.398
2.582GlnSer: 2.582 ± 0.639
2.09GlnThr: 2.09 ± 0.354
3.012GlnVal: 3.012 ± 0.501
1.045GlnTrp: 1.045 ± 0.281
1.168GlnTyr: 1.168 ± 0.266
0.0GlnXaa: 0.0 ± 0.0
Arg
4.61ArgAla: 4.61 ± 0.504
0.738ArgCys: 0.738 ± 0.194
3.258ArgAsp: 3.258 ± 0.416
2.766ArgGlu: 2.766 ± 0.411
2.52ArgPhe: 2.52 ± 0.386
3.258ArgGly: 3.258 ± 0.461
0.922ArgHis: 0.922 ± 0.243
3.196ArgIle: 3.196 ± 0.523
2.889ArgLys: 2.889 ± 0.496
4.795ArgLeu: 4.795 ± 0.547
1.475ArgMet: 1.475 ± 0.291
2.828ArgAsn: 2.828 ± 0.356
2.705ArgPro: 2.705 ± 0.387
1.844ArgGln: 1.844 ± 0.374
2.09ArgArg: 2.09 ± 0.478
2.951ArgSer: 2.951 ± 0.364
2.705ArgThr: 2.705 ± 0.459
3.75ArgVal: 3.75 ± 0.456
1.168ArgTrp: 1.168 ± 0.32
2.029ArgTyr: 2.029 ± 0.333
0.0ArgXaa: 0.0 ± 0.0
Ser
4.979SerAla: 4.979 ± 0.524
0.369SerCys: 0.369 ± 0.146
3.135SerAsp: 3.135 ± 0.441
2.889SerGlu: 2.889 ± 0.379
1.66SerPhe: 1.66 ± 0.268
5.286SerGly: 5.286 ± 0.539
0.738SerHis: 0.738 ± 0.221
4.364SerIle: 4.364 ± 0.573
2.705SerLys: 2.705 ± 0.463
4.303SerLeu: 4.303 ± 0.516
1.352SerMet: 1.352 ± 0.275
2.766SerAsn: 2.766 ± 0.41
2.029SerPro: 2.029 ± 0.338
2.951SerGln: 2.951 ± 0.454
2.151SerArg: 2.151 ± 0.304
3.012SerSer: 3.012 ± 0.454
3.196SerThr: 3.196 ± 0.416
4.918SerVal: 4.918 ± 0.586
0.553SerTrp: 0.553 ± 0.201
1.844SerTyr: 1.844 ± 0.332
0.0SerXaa: 0.0 ± 0.0
Thr
4.672ThrAla: 4.672 ± 0.61
0.861ThrCys: 0.861 ± 0.271
4.241ThrAsp: 4.241 ± 0.507
3.258ThrGlu: 3.258 ± 0.4
1.967ThrPhe: 1.967 ± 0.456
6.639ThrGly: 6.639 ± 0.786
0.799ThrHis: 0.799 ± 0.216
3.074ThrIle: 3.074 ± 0.432
3.074ThrLys: 3.074 ± 0.446
5.717ThrLeu: 5.717 ± 0.604
1.291ThrMet: 1.291 ± 0.38
3.319ThrAsn: 3.319 ± 0.453
4.487ThrPro: 4.487 ± 0.635
1.967ThrGln: 1.967 ± 0.362
3.319ThrArg: 3.319 ± 0.45
2.951ThrSer: 2.951 ± 0.455
4.733ThrThr: 4.733 ± 0.678
5.655ThrVal: 5.655 ± 0.6
0.615ThrTrp: 0.615 ± 0.231
2.213ThrTyr: 2.213 ± 0.379
0.0ThrXaa: 0.0 ± 0.0
Val
6.209ValAla: 6.209 ± 1.156
0.676ValCys: 0.676 ± 0.218
4.979ValAsp: 4.979 ± 0.616
4.119ValGlu: 4.119 ± 0.626
3.012ValPhe: 3.012 ± 0.416
4.733ValGly: 4.733 ± 0.587
1.414ValHis: 1.414 ± 0.296
4.057ValIle: 4.057 ± 0.476
4.119ValLys: 4.119 ± 0.463
5.778ValLeu: 5.778 ± 0.555
1.721ValMet: 1.721 ± 0.344
4.61ValAsn: 4.61 ± 0.542
2.459ValPro: 2.459 ± 0.389
2.889ValGln: 2.889 ± 0.46
3.074ValArg: 3.074 ± 0.343
3.811ValSer: 3.811 ± 0.396
5.655ValThr: 5.655 ± 0.835
3.996ValVal: 3.996 ± 0.533
0.799ValTrp: 0.799 ± 0.213
2.09ValTyr: 2.09 ± 0.283
0.0ValXaa: 0.0 ± 0.0
Trp
0.861TrpAla: 0.861 ± 0.24
0.184TrpCys: 0.184 ± 0.114
1.045TrpAsp: 1.045 ± 0.256
1.168TrpGlu: 1.168 ± 0.248
0.799TrpPhe: 0.799 ± 0.242
1.168TrpGly: 1.168 ± 0.254
0.246TrpHis: 0.246 ± 0.119
0.799TrpIle: 0.799 ± 0.245
1.168TrpLys: 1.168 ± 0.317
1.537TrpLeu: 1.537 ± 0.286
0.492TrpMet: 0.492 ± 0.167
1.168TrpAsn: 1.168 ± 0.289
0.43TrpPro: 0.43 ± 0.177
0.553TrpGln: 0.553 ± 0.175
0.861TrpArg: 0.861 ± 0.248
1.045TrpSer: 1.045 ± 0.299
0.676TrpThr: 0.676 ± 0.21
1.168TrpVal: 1.168 ± 0.193
0.553TrpTrp: 0.553 ± 0.207
0.861TrpTyr: 0.861 ± 0.251
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.135TyrAla: 3.135 ± 0.721
0.799TyrCys: 0.799 ± 0.203
2.151TyrAsp: 2.151 ± 0.355
2.09TyrGlu: 2.09 ± 0.377
1.168TyrPhe: 1.168 ± 0.281
2.213TyrGly: 2.213 ± 0.35
0.553TyrHis: 0.553 ± 0.191
2.151TyrIle: 2.151 ± 0.414
2.09TyrLys: 2.09 ± 0.414
1.906TyrLeu: 1.906 ± 0.419
1.168TyrMet: 1.168 ± 0.254
2.09TyrAsn: 2.09 ± 0.4
1.598TyrPro: 1.598 ± 0.324
2.029TyrGln: 2.029 ± 0.386
2.643TyrArg: 2.643 ± 0.446
2.09TyrSer: 2.09 ± 0.311
2.459TyrThr: 2.459 ± 0.391
2.828TyrVal: 2.828 ± 0.339
0.615TyrTrp: 0.615 ± 0.166
1.352TyrTyr: 1.352 ± 0.327
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 84 proteins (16269 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski