Halomonas aestuarii

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Halomonadaceae; Halomonas

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3147 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1J0VEY9|A0A1J0VEY9_9GAMM tRNA(Met) cytidine acetyltransferase TmcA OS=Halomonas aestuarii OX=1897729 GN=tmcA PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 10.48RR3 pKa = 11.84LIATAALASLVASPAFAQSYY23 pKa = 9.4QYY25 pKa = 10.87TPIEE29 pKa = 4.18GPYY32 pKa = 9.53LGGAIGHH39 pKa = 7.13ASLDD43 pKa = 3.61SDD45 pKa = 3.76TLNEE49 pKa = 4.04LEE51 pKa = 4.62EE52 pKa = 5.42LGLDD56 pKa = 3.73TDD58 pKa = 5.55DD59 pKa = 5.74SDD61 pKa = 3.77TAYY64 pKa = 10.17KK65 pKa = 10.63LFAGYY70 pKa = 10.57RR71 pKa = 11.84FTPNFAMEE79 pKa = 4.48ASYY82 pKa = 11.53LDD84 pKa = 4.12FGDD87 pKa = 5.51FSADD91 pKa = 2.99ATFTNGTDD99 pKa = 2.94TATADD104 pKa = 4.45LEE106 pKa = 4.6GGLDD110 pKa = 3.89GVGLALVGKK119 pKa = 10.08LPIQGGLSVHH129 pKa = 6.48GKK131 pKa = 10.27LGMIAWDD138 pKa = 3.77GDD140 pKa = 3.7VSGTARR146 pKa = 11.84LNGEE150 pKa = 4.28VIYY153 pKa = 10.93DD154 pKa = 4.12GAAGEE159 pKa = 4.94DD160 pKa = 3.99GTDD163 pKa = 3.24PFYY166 pKa = 11.2GIGAEE171 pKa = 4.13YY172 pKa = 10.1EE173 pKa = 4.13IQQVMIRR180 pKa = 11.84GEE182 pKa = 4.01YY183 pKa = 9.2EE184 pKa = 3.78RR185 pKa = 11.84YY186 pKa = 10.07DD187 pKa = 3.39ISDD190 pKa = 3.39SGEE193 pKa = 3.96DD194 pKa = 3.65FEE196 pKa = 7.17IDD198 pKa = 3.95LFSASLGYY206 pKa = 10.47RR207 pKa = 11.84FF208 pKa = 4.6

Molecular weight: 22.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1J0VFV6|A0A1J0VFV6_9GAMM UPF0301 protein BOX17_08120 OS=Halomonas aestuarii OX=1897729 GN=BOX17_08120 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.34RR12 pKa = 11.84KK13 pKa = 9.1RR14 pKa = 11.84VHH16 pKa = 6.26GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.3NGRR28 pKa = 11.84AVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.93GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3147

0

3147

1029740

37

2524

327.2

35.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.597 ± 0.051

0.895 ± 0.018

5.917 ± 0.044

6.733 ± 0.05

3.386 ± 0.029

8.632 ± 0.043

2.494 ± 0.026

4.331 ± 0.036

2.275 ± 0.033

11.756 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.479 ± 0.023

2.186 ± 0.021

5.175 ± 0.028

3.429 ± 0.025

7.802 ± 0.055

4.989 ± 0.032

4.913 ± 0.039

7.36 ± 0.032

1.48 ± 0.019

2.172 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski