Mycoplasma citelli
Average proteome isoelectric point is 7.46
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 908 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A449B2R3|A0A449B2R3_9MOLU Uracil-DNA glycosylase OS=Mycoplasma citelli OX=171281 GN=ung PE=3 SV=1
MM1 pKa = 6.88 NTILIQSQGTKK12 pKa = 10.07 NLAYY16 pKa = 10.28 VQEE19 pKa = 4.34 KK20 pKa = 10.66 VDD22 pKa = 4.2 FNVKK26 pKa = 10.48 DD27 pKa = 4.06 FLSKK31 pKa = 10.99 LDD33 pKa = 3.9 MEE35 pKa = 4.99 IKK37 pKa = 9.4 TLSIEE42 pKa = 4.02 EE43 pKa = 3.97 QKK45 pKa = 11.31 DD46 pKa = 3.79 YY47 pKa = 11.11 ISKK50 pKa = 10.61 CEE52 pKa = 3.6 YY53 pKa = 10.01 LINYY57 pKa = 7.73 KK58 pKa = 10.27 FQDD61 pKa = 3.68 PNQGVLVFQILDD73 pKa = 3.49 LDD75 pKa = 3.81 YY76 pKa = 11.26 SYY78 pKa = 12.02 DD79 pKa = 3.77 NLTEE83 pKa = 3.81 IYY85 pKa = 10.27 YY86 pKa = 10.14 ILDD89 pKa = 3.5 EE90 pKa = 4.76 EE91 pKa = 4.64 FDD93 pKa = 3.79 DD94 pKa = 4.0 TRR96 pKa = 11.84 TFSSFSEE103 pKa = 4.0 NNPDD107 pKa = 4.16 IKK109 pKa = 10.81 AGCDD113 pKa = 2.85 WYY115 pKa = 10.09 DD116 pKa = 3.5 TLDD119 pKa = 4.26 KK120 pKa = 11.03 SLQEE124 pKa = 4.21 YY125 pKa = 10.32 GLQSKK130 pKa = 9.26 YY131 pKa = 11.08 AYY133 pKa = 7.8 LTNEE137 pKa = 3.68 ILEE140 pKa = 4.25 LQGDD144 pKa = 3.82 KK145 pKa = 10.92 NYY147 pKa = 10.81 YY148 pKa = 9.94 FLPDD152 pKa = 3.45 YY153 pKa = 10.69 DD154 pKa = 4.65 SPLKK158 pKa = 10.68 ALSSTEE164 pKa = 4.28 LINLIFTFMLNLKK177 pKa = 8.37 QQNQQFKK184 pKa = 9.8 EE185 pKa = 4.04
Molecular weight: 21.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.877
IPC2_protein 4.139
IPC_protein 4.113
Toseland 3.91
ProMoST 4.215
Dawson 4.075
Bjellqvist 4.266
Wikipedia 3.986
Rodwell 3.935
Grimsley 3.821
Solomon 4.075
Lehninger 4.037
Nozaki 4.19
DTASelect 4.406
Thurlkill 3.948
EMBOSS 3.999
Sillero 4.228
Patrickios 1.952
IPC_peptide 4.075
IPC2_peptide 4.202
IPC2.peptide.svr19 4.123
Protein with the highest isoelectric point:
>tr|A0A449B2B3|A0A449B2B3_9MOLU Uncharacterized protein OS=Mycoplasma citelli OX=171281 GN=NCTC10181_00592 PE=4 SV=1
MM1 pKa = 7.07 FVSNKK6 pKa = 9.75 SRR8 pKa = 11.84 FSLVIFSFFLGAFEE22 pKa = 4.93 IDD24 pKa = 2.76 RR25 pKa = 11.84 FYY27 pKa = 11.48 AGRR30 pKa = 11.84 FALGLLKK37 pKa = 10.78 LFTVSSFGLWWFIDD51 pKa = 3.85 FVLALTGRR59 pKa = 11.84 MRR61 pKa = 11.84 DD62 pKa = 3.6 EE63 pKa = 4.04 NGLRR67 pKa = 11.84 ISRR70 pKa = 11.84 WW71 pKa = 3.28
Molecular weight: 8.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.326
IPC2_protein 9.677
IPC_protein 10.804
Toseland 10.935
ProMoST 10.965
Dawson 10.979
Bjellqvist 10.804
Wikipedia 11.301
Rodwell 10.979
Grimsley 11.023
Solomon 11.257
Lehninger 11.199
Nozaki 10.906
DTASelect 10.804
Thurlkill 10.921
EMBOSS 11.359
Sillero 10.935
Patrickios 10.833
IPC_peptide 11.272
IPC2_peptide 9.926
IPC2.peptide.svr19 8.804
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
908
0
908
369893
29
4092
407.4
46.7
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.307 ± 0.102
0.335 ± 0.017
5.426 ± 0.06
6.534 ± 0.069
5.419 ± 0.086
4.129 ± 0.083
1.314 ± 0.03
8.728 ± 0.089
10.243 ± 0.083
10.231 ± 0.077
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.349 ± 0.04
8.544 ± 0.137
2.664 ± 0.048
4.577 ± 0.082
2.536 ± 0.052
7.152 ± 0.079
5.272 ± 0.088
5.179 ± 0.067
0.896 ± 0.029
4.164 ± 0.062
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here