Penicillium janczewskii chrysovirus 2
Average proteome isoelectric point is 5.88
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0S2KPW5|A0A0S2KPW5_9VIRU RNA-directed RNA polymerase OS=Penicillium janczewskii chrysovirus 2 OX=1755793 PE=4 SV=1
MM1 pKa = 7.63 SSPLASPSAILGRR14 pKa = 11.84 DD15 pKa = 3.53 VPSLISSWADD25 pKa = 3.19 EE26 pKa = 4.26 VEE28 pKa = 3.86 EE29 pKa = 4.95 AYY31 pKa = 10.54 ASGEE35 pKa = 4.05 MATVEE40 pKa = 4.55 DD41 pKa = 4.11 GRR43 pKa = 11.84 GAAGAASLVRR53 pKa = 11.84 ADD55 pKa = 5.12 AILSGTTAWGPEE67 pKa = 3.69 SAARR71 pKa = 11.84 SGLGATTLARR81 pKa = 11.84 ATGLRR86 pKa = 11.84 RR87 pKa = 11.84 TTGAVFPPEE96 pKa = 4.56 CEE98 pKa = 4.62 DD99 pKa = 3.62 GGHH102 pKa = 6.81 LCGIPFGPRR111 pKa = 11.84 VFAATWVDD119 pKa = 2.88 ADD121 pKa = 3.32 IAYY124 pKa = 10.02 GSSEE128 pKa = 3.7 PMRR131 pKa = 11.84 RR132 pKa = 11.84 CSDD135 pKa = 3.18 NAIRR139 pKa = 11.84 SLLRR143 pKa = 11.84 TLDD146 pKa = 3.5 GSEE149 pKa = 4.3 GAFTLPSEE157 pKa = 4.68 TRR159 pKa = 11.84 CQVYY163 pKa = 10.14 RR164 pKa = 11.84 SMDD167 pKa = 2.98 QHH169 pKa = 6.67 MYY171 pKa = 9.99 LQPDD175 pKa = 3.36 EE176 pKa = 4.66 GFFWVTFQVRR186 pKa = 11.84 VEE188 pKa = 4.07 AQLMVYY194 pKa = 7.91 GHH196 pKa = 7.38 PDD198 pKa = 2.74 GRR200 pKa = 11.84 IRR202 pKa = 11.84 DD203 pKa = 3.72 VAVARR208 pKa = 11.84 DD209 pKa = 3.72 LRR211 pKa = 11.84 AMRR214 pKa = 11.84 SADD217 pKa = 3.08 VSAYY221 pKa = 10.45 RR222 pKa = 11.84 DD223 pKa = 3.74 MLVVLAGIQQAIVFGEE239 pKa = 4.44 LGCVLTVLHH248 pKa = 7.13 DD249 pKa = 4.11 CPLGEE254 pKa = 4.54 IKK256 pKa = 10.56 QSVHH260 pKa = 4.89 RR261 pKa = 11.84 HH262 pKa = 5.18 PGGGVVTAGHH272 pKa = 6.51 TGSPVQILDD281 pKa = 3.94 TSGTWLPTPAQLHH294 pKa = 5.3 HH295 pKa = 6.34 QRR297 pKa = 11.84 VSAATSFPEE306 pKa = 3.97 GTVAHH311 pKa = 6.07 GVVSASEE318 pKa = 4.04 VMPTLVVGLGANGGKK333 pKa = 9.78 SLTGSVLCVRR343 pKa = 11.84 VLEE346 pKa = 4.64 AGFAGARR353 pKa = 11.84 NLVTTSTTANAPLPMFKK370 pKa = 9.71 RR371 pKa = 11.84 GMALAPCARR380 pKa = 11.84 DD381 pKa = 3.09 GLLSHH386 pKa = 6.91 TCAAALYY393 pKa = 9.08 GDD395 pKa = 3.78 GLGGVFVYY403 pKa = 10.58 PDD405 pKa = 3.79 GVRR408 pKa = 11.84 VAVDD412 pKa = 3.44 TVLSKK417 pKa = 10.88 LRR419 pKa = 11.84 SDD421 pKa = 3.34 GHH423 pKa = 6.5 AWVYY427 pKa = 11.07 ARR429 pKa = 11.84 DD430 pKa = 3.79 VVATKK435 pKa = 10.37 QYY437 pKa = 10.9 VLDD440 pKa = 5.63 LISLQQGWDD449 pKa = 3.3 RR450 pKa = 11.84 QLDD453 pKa = 3.56 NDD455 pKa = 4.37 GAITVLDD462 pKa = 3.74 SRR464 pKa = 11.84 RR465 pKa = 11.84 EE466 pKa = 4.18 IIGSVYY472 pKa = 10.24 VLPGAGSQPRR482 pKa = 11.84 WATVLEE488 pKa = 4.73 GVTMPTKK495 pKa = 10.47 CGSEE499 pKa = 3.74 LWLDD503 pKa = 3.65 HH504 pKa = 6.27 GVSGLGRR511 pKa = 11.84 YY512 pKa = 8.89 LLANHH517 pKa = 6.33 ITNGRR522 pKa = 11.84 FTSALSRR529 pKa = 11.84 LAFNWKK535 pKa = 10.36 GPFQASTAAVSAAKK549 pKa = 9.48 TLGVSAGLQLPISYY563 pKa = 10.45 VVDD566 pKa = 3.36 TEE568 pKa = 4.47 FISRR572 pKa = 11.84 KK573 pKa = 10.23 GPDD576 pKa = 3.66 GNLDD580 pKa = 3.3 RR581 pKa = 11.84 YY582 pKa = 9.82 VFAVGVARR590 pKa = 11.84 FHH592 pKa = 7.34 DD593 pKa = 4.35 GQYY596 pKa = 9.4 QGSMTVVDD604 pKa = 4.75 TSAEE608 pKa = 4.0 FHH610 pKa = 6.53 EE611 pKa = 5.0 FAAANRR617 pKa = 11.84 SKK619 pKa = 10.75 LGRR622 pKa = 11.84 HH623 pKa = 4.19 MASWEE628 pKa = 3.94 VLQRR632 pKa = 11.84 GAKK635 pKa = 10.01 HH636 pKa = 6.3 GDD638 pKa = 3.2 PTVVLEE644 pKa = 4.19 RR645 pKa = 11.84 LRR647 pKa = 11.84 EE648 pKa = 3.95 VAADD652 pKa = 3.58 TNVRR656 pKa = 11.84 VYY658 pKa = 11.32 AKK660 pKa = 10.44 GYY662 pKa = 9.48 DD663 pKa = 3.62 AEE665 pKa = 4.57 SEE667 pKa = 4.25 ILVSEE672 pKa = 4.17 PVGATRR678 pKa = 11.84 LFRR681 pKa = 11.84 RR682 pKa = 11.84 QAGNRR687 pKa = 11.84 APLRR691 pKa = 11.84 EE692 pKa = 4.42 LGLLTRR698 pKa = 11.84 GYY700 pKa = 11.04 DD701 pKa = 3.37 EE702 pKa = 4.64 LARR705 pKa = 11.84 EE706 pKa = 4.36 IGWPTDD712 pKa = 3.28 HH713 pKa = 7.9 DD714 pKa = 4.14 PGRR717 pKa = 11.84 EE718 pKa = 4.05 CTLFGYY724 pKa = 7.7 TAGLCDD730 pKa = 3.92 SLPPLRR736 pKa = 11.84 EE737 pKa = 3.71 IGNDD741 pKa = 3.5 SIHH744 pKa = 6.69 AVLGSMSQAGILL756 pKa = 3.62
Molecular weight: 80.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.745
IPC2_protein 5.664
IPC_protein 5.728
Toseland 6.135
ProMoST 6.084
Dawson 5.995
Bjellqvist 6.008
Wikipedia 6.008
Rodwell 5.982
Grimsley 6.287
Solomon 5.995
Lehninger 5.995
Nozaki 6.262
DTASelect 6.465
Thurlkill 6.465
EMBOSS 6.427
Sillero 6.364
Patrickios 4.139
IPC_peptide 6.008
IPC2_peptide 6.389
IPC2.peptide.svr19 6.262
Protein with the highest isoelectric point:
>tr|A0A0S2KQ51|A0A0S2KQ51_9VIRU ORF2 OS=Penicillium janczewskii chrysovirus 2 OX=1755793 PE=4 SV=1
MM1 pKa = 6.96 NTPTLGASYY10 pKa = 10.09 TRR12 pKa = 11.84 TPTGGNSTRR21 pKa = 11.84 LFASDD26 pKa = 3.62 NDD28 pKa = 3.86 AKK30 pKa = 10.88 LKK32 pKa = 10.0 EE33 pKa = 4.33 WEE35 pKa = 4.18 GLHH38 pKa = 7.16 AIVLPTCCGKK48 pKa = 10.69 SSAALRR54 pKa = 11.84 FGGYY58 pKa = 10.45 DD59 pKa = 3.01 IDD61 pKa = 6.58 DD62 pKa = 3.86 IVADD66 pKa = 4.17 ASIDD70 pKa = 3.84 EE71 pKa = 5.36 LDD73 pKa = 3.92 TEE75 pKa = 4.68 LDD77 pKa = 3.26 AMLGARR83 pKa = 11.84 EE84 pKa = 4.51 DD85 pKa = 4.18 GVHH88 pKa = 6.48 GNDD91 pKa = 2.92 SSAMHH96 pKa = 6.76 RR97 pKa = 11.84 SNRR100 pKa = 11.84 LMLMRR105 pKa = 11.84 ARR107 pKa = 11.84 RR108 pKa = 11.84 FFATVSPDD116 pKa = 3.54 DD117 pKa = 3.95 NPLVVYY123 pKa = 9.53 CHH125 pKa = 5.77 TAEE128 pKa = 4.13 FAQALGVPVGLVVHH142 pKa = 6.88 LDD144 pKa = 3.54 EE145 pKa = 4.98 EE146 pKa = 4.69 AVSNSIRR153 pKa = 11.84 MTRR156 pKa = 11.84 EE157 pKa = 3.27 TTPEE161 pKa = 3.71 VRR163 pKa = 11.84 NVTLQVYY170 pKa = 9.54 RR171 pKa = 11.84 EE172 pKa = 3.8 QTAANRR178 pKa = 11.84 EE179 pKa = 3.88 FSRR182 pKa = 11.84 RR183 pKa = 11.84 HH184 pKa = 4.97 GLSSRR189 pKa = 11.84 DD190 pKa = 3.59 CYY192 pKa = 11.15 SYY194 pKa = 11.67 SDD196 pKa = 3.68 VQQCVWSALVRR207 pKa = 11.84 CAVLAPCRR215 pKa = 11.84 QGDD218 pKa = 4.18 EE219 pKa = 4.1 YY220 pKa = 11.33 HH221 pKa = 7.08 AMLDD225 pKa = 3.95 SRR227 pKa = 11.84 PQLSDD232 pKa = 3.11 VLNRR236 pKa = 11.84 SHH238 pKa = 6.91 AVVRR242 pKa = 11.84 TTSTPNWVRR251 pKa = 11.84 ACAARR256 pKa = 11.84 QLKK259 pKa = 10.62 LSLGDD264 pKa = 3.83 LAPTEE269 pKa = 3.79 AHH271 pKa = 6.74 AAHH274 pKa = 6.64 SHH276 pKa = 6.0 PAWASIVHH284 pKa = 6.15 IVASQTGHH292 pKa = 5.95 VPEE295 pKa = 4.13 QAYY298 pKa = 10.54 ASIPDD303 pKa = 3.34 WSEE306 pKa = 3.99 EE307 pKa = 3.54 EE308 pKa = 3.72 WRR310 pKa = 11.84 EE311 pKa = 3.91 YY312 pKa = 10.99 FPLGPGNASFALVNVSDD329 pKa = 3.76 WLARR333 pKa = 11.84 TPSDD337 pKa = 3.22 ALRR340 pKa = 11.84 STAYY344 pKa = 9.88 SWFRR348 pKa = 11.84 QLLLVRR354 pKa = 11.84 GTKK357 pKa = 8.56 YY358 pKa = 10.51 EE359 pKa = 4.09 RR360 pKa = 11.84 LLCMLVMGDD369 pKa = 3.33 VSHH372 pKa = 6.58 YY373 pKa = 9.95 VAPQYY378 pKa = 10.77 TEE380 pKa = 4.14 LYY382 pKa = 10.41 SRR384 pKa = 11.84 IPLGLLSDD392 pKa = 3.82 VVYY395 pKa = 9.18 ATISKK400 pKa = 9.85 QIHH403 pKa = 5.09 AAVRR407 pKa = 11.84 VGCNYY412 pKa = 10.26 LGQRR416 pKa = 11.84 IPSRR420 pKa = 11.84 DD421 pKa = 3.35 LTYY424 pKa = 11.0 FMYY427 pKa = 10.45 FDD429 pKa = 4.35 CLAGRR434 pKa = 11.84 VIGTEE439 pKa = 4.49 DD440 pKa = 3.41 IDD442 pKa = 5.26 AEE444 pKa = 4.17 IADD447 pKa = 4.41 RR448 pKa = 11.84 TRR450 pKa = 11.84 LQSPKK455 pKa = 9.94 KK456 pKa = 9.48 FCLGGEE462 pKa = 3.98 WSEE465 pKa = 4.81 SEE467 pKa = 3.65 FDD469 pKa = 3.63 NRR471 pKa = 11.84 FTGAVQKK478 pKa = 10.81 AYY480 pKa = 10.89 DD481 pKa = 4.33 HH482 pKa = 6.27 LTQGLGAKK490 pKa = 9.16 LRR492 pKa = 11.84 AMTNVIDD499 pKa = 4.14 TFDD502 pKa = 3.27 HH503 pKa = 6.2 FMEE506 pKa = 4.79 EE507 pKa = 3.3 RR508 pKa = 11.84 RR509 pKa = 11.84 TWVRR513 pKa = 11.84 PGAASGAPKK522 pKa = 10.18 ADD524 pKa = 3.49 LMLEE528 pKa = 4.47 VPASQAEE535 pKa = 4.06 ALDD538 pKa = 5.01 AISTDD543 pKa = 3.6 LGHH546 pKa = 5.74 MTVLILRR553 pKa = 11.84 RR554 pKa = 11.84 VRR556 pKa = 11.84 LNKK559 pKa = 10.09 SAVFEE564 pKa = 4.3 FPQFVDD570 pKa = 3.8 TVKK573 pKa = 10.57 EE574 pKa = 3.82 ALRR577 pKa = 11.84 DD578 pKa = 3.67 YY579 pKa = 10.92 VPNSFTKK586 pKa = 10.69 YY587 pKa = 9.59 FFKK590 pKa = 11.0 FEE592 pKa = 4.44 PGKK595 pKa = 9.95 FASRR599 pKa = 11.84 ALFPSHH605 pKa = 6.95 LLHH608 pKa = 7.26 YY609 pKa = 10.71 IMVSHH614 pKa = 6.42 VLFVAEE620 pKa = 4.37 KK621 pKa = 10.26 GGVIPEE627 pKa = 4.22 TRR629 pKa = 11.84 LTAGPEE635 pKa = 4.05 AQQEE639 pKa = 4.29 DD640 pKa = 3.52 HH641 pKa = 6.22 WLWRR645 pKa = 11.84 EE646 pKa = 3.71 MHH648 pKa = 6.49 DD649 pKa = 3.82 TAVHH653 pKa = 6.79 LMLDD657 pKa = 3.45 YY658 pKa = 11.66 ANFNEE663 pKa = 4.21 QHH665 pKa = 6.41 SIKK668 pKa = 10.48 HH669 pKa = 4.61 MQATILGLKK678 pKa = 9.58 GVYY681 pKa = 9.66 SKK683 pKa = 10.88 HH684 pKa = 6.81 LSLTPDD690 pKa = 4.02 LARR693 pKa = 11.84 AIDD696 pKa = 3.53 WTVEE700 pKa = 3.81 SFEE703 pKa = 4.92 RR704 pKa = 11.84 ICAEE708 pKa = 3.58 QDD710 pKa = 2.97 GKK712 pKa = 10.69 LVRR715 pKa = 11.84 FTHH718 pKa = 6.49 GLLSGWRR725 pKa = 11.84 CTTWVNSIANVAYY738 pKa = 10.59 LDD740 pKa = 4.05 VIRR743 pKa = 11.84 QQVQEE748 pKa = 3.94 LTGRR752 pKa = 11.84 SVLVRR757 pKa = 11.84 TQSGGDD763 pKa = 3.27 DD764 pKa = 3.49 VAAEE768 pKa = 4.17 EE769 pKa = 4.57 CSLYY773 pKa = 10.93 DD774 pKa = 3.14 AALTLRR780 pKa = 11.84 VGEE783 pKa = 4.12 VMGFEE788 pKa = 4.67 FKK790 pKa = 10.58 AIKK793 pKa = 10.04 QLISCEE799 pKa = 3.65 YY800 pKa = 10.47 RR801 pKa = 11.84 EE802 pKa = 4.13 FFRR805 pKa = 11.84 LFITRR810 pKa = 11.84 EE811 pKa = 3.81 GVYY814 pKa = 10.89 GSLCRR819 pKa = 11.84 MLGSALSGQWSNSVLPKK836 pKa = 10.32 LVEE839 pKa = 4.16 PATKK843 pKa = 10.21 LSSVVEE849 pKa = 4.19 IARR852 pKa = 11.84 KK853 pKa = 8.86 AGRR856 pKa = 11.84 RR857 pKa = 11.84 ARR859 pKa = 11.84 NLNLMEE865 pKa = 4.58 KK866 pKa = 8.67 MALVAFDD873 pKa = 3.41 KK874 pKa = 10.57 WATTGEE880 pKa = 4.06 EE881 pKa = 4.37 CLVDD885 pKa = 4.88 FYY887 pKa = 12.21 VHH889 pKa = 5.73 GTKK892 pKa = 8.79 EE893 pKa = 4.21 TGGLGIPNVTGDD905 pKa = 4.44 LYY907 pKa = 11.76 VLDD910 pKa = 4.99 GSPDD914 pKa = 3.32 EE915 pKa = 5.31 RR916 pKa = 11.84 PTHH919 pKa = 6.28 LKK921 pKa = 10.33 PVGHH925 pKa = 7.25 PSDD928 pKa = 3.89 ASSVTAARR936 pKa = 11.84 IVDD939 pKa = 3.73 KK940 pKa = 10.79 AVSLVGPEE948 pKa = 4.05 AVLPTATVASTMAEE962 pKa = 4.11 GAFVSSIAQNLGPRR976 pKa = 11.84 ALRR979 pKa = 11.84 VGGRR983 pKa = 11.84 RR984 pKa = 11.84 QSHH987 pKa = 5.06 KK988 pKa = 9.55 VVRR991 pKa = 11.84 VLRR994 pKa = 11.84 IRR996 pKa = 11.84 EE997 pKa = 3.77 EE998 pKa = 4.02 DD999 pKa = 3.63 VAPGRR1004 pKa = 11.84 TSADD1008 pKa = 3.53 YY1009 pKa = 10.6 QHH1011 pKa = 6.84 SKK1013 pKa = 10.4 LALRR1017 pKa = 11.84 VQLDD1021 pKa = 3.17 AMKK1024 pKa = 10.55 RR1025 pKa = 11.84 AGRR1028 pKa = 11.84 RR1029 pKa = 11.84 YY1030 pKa = 10.38 AEE1032 pKa = 4.49 LAPAVKK1038 pKa = 9.24 PAKK1041 pKa = 10.28 KK1042 pKa = 9.98 LDD1044 pKa = 3.56 LARR1047 pKa = 11.84 SVGASEE1053 pKa = 5.12 GVGGDD1058 pKa = 3.87 LLYY1061 pKa = 10.78 YY1062 pKa = 8.6 WQEE1065 pKa = 3.56 EE1066 pKa = 4.3 HH1067 pKa = 6.88 VLYY1070 pKa = 11.07 GCATYY1075 pKa = 10.25 MLTEE1079 pKa = 5.05 DD1080 pKa = 4.0 YY1081 pKa = 11.29 YY1082 pKa = 11.31 EE1083 pKa = 4.37 DD1084 pKa = 3.6 VVLIGLLQRR1093 pKa = 11.84 GRR1095 pKa = 11.84 DD1096 pKa = 3.27 RR1097 pKa = 11.84 CAVANRR1103 pKa = 11.84 CAEE1106 pKa = 4.02 LAMGLRR1112 pKa = 11.84 NDD1114 pKa = 3.47 GYY1116 pKa = 9.79 MWYY1119 pKa = 10.56
Molecular weight: 124.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.002
IPC2_protein 5.957
IPC_protein 6.033
Toseland 6.402
ProMoST 6.44
Dawson 6.351
Bjellqvist 6.351
Wikipedia 6.376
Rodwell 6.351
Grimsley 6.547
Solomon 6.351
Lehninger 6.351
Nozaki 6.634
DTASelect 6.825
Thurlkill 6.839
EMBOSS 6.81
Sillero 6.737
Patrickios 4.139
IPC_peptide 6.376
IPC2_peptide 6.707
IPC2.peptide.svr19 6.652
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
3313
655
1119
828.3
89.73
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.651 ± 0.537
1.57 ± 0.132
5.403 ± 0.233
5.343 ± 0.314
2.656 ± 0.286
8.391 ± 0.926
2.777 ± 0.13
3.049 ± 0.037
2.324 ± 0.384
9.81 ± 0.329
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.264 ± 0.122
2.203 ± 0.187
5.312 ± 0.639
3.441 ± 0.305
7.274 ± 0.167
7.486 ± 0.433
6.007 ± 0.186
8.421 ± 0.228
1.6 ± 0.056
3.018 ± 0.431
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here