Nakamurella flava

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Nakamurellales; Nakamurellaceae; Nakamurella

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4268 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4U6QLE3|A0A4U6QLE3_9ACTN SLT domain-containing protein OS=Nakamurella flava OX=2576308 GN=FDO65_07025 PE=4 SV=1
MM1 pKa = 7.16TFSTVSLRR9 pKa = 11.84VFTAGVALAAACGLAACGGTTAGTAAPAPAPATVTVTASSSAASASTSARR59 pKa = 11.84PGGDD63 pKa = 2.85SSGSASTSARR73 pKa = 11.84PTRR76 pKa = 11.84PSSTPSAEE84 pKa = 3.66PSEE87 pKa = 4.75FEE89 pKa = 4.39TVDD92 pKa = 3.18PTVDD96 pKa = 3.01VGTVDD101 pKa = 3.01QATAVWLDD109 pKa = 3.82TACTDD114 pKa = 3.56LTTLLPVLLAVPTLDD129 pKa = 3.2QTTTPVEE136 pKa = 4.3EE137 pKa = 4.08FRR139 pKa = 11.84TAYY142 pKa = 9.75RR143 pKa = 11.84DD144 pKa = 3.66YY145 pKa = 10.34YY146 pKa = 10.47ATVADD151 pKa = 3.84TALQVNGNLVGIDD164 pKa = 3.85PPAVTDD170 pKa = 4.81GPALHH175 pKa = 6.84EE176 pKa = 4.56AYY178 pKa = 9.94QYY180 pKa = 10.07YY181 pKa = 10.64LKK183 pKa = 10.62RR184 pKa = 11.84LADD187 pKa = 3.47ITGSGSIAIDD197 pKa = 3.57EE198 pKa = 4.5ATDD201 pKa = 3.24IAGIQAVVEE210 pKa = 4.17QIQFEE215 pKa = 4.29IDD217 pKa = 3.21GLSQEE222 pKa = 5.43DD223 pKa = 3.22IGLNSLSGAEE233 pKa = 3.97LQQYY237 pKa = 7.44MKK239 pKa = 10.47QIPACSEE246 pKa = 4.03FVTSS250 pKa = 4.41

Molecular weight: 25.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4U6QL53|A0A4U6QL53_9ACTN Polyphosphate kinase 2 OS=Nakamurella flava OX=2576308 GN=ppk2 PE=3 SV=1
MM1 pKa = 7.28TSNTITRR8 pKa = 11.84GSRR11 pKa = 11.84IGRR14 pKa = 11.84GSRR17 pKa = 11.84IGRR20 pKa = 11.84GSRR23 pKa = 11.84IGRR26 pKa = 11.84GSRR29 pKa = 11.84IGRR32 pKa = 11.84GSRR35 pKa = 11.84IGG37 pKa = 3.32

Molecular weight: 3.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4268

0

4268

1432224

26

1837

335.6

35.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.867 ± 0.05

0.676 ± 0.01

6.42 ± 0.033

4.612 ± 0.031

2.725 ± 0.021

9.571 ± 0.039

1.954 ± 0.02

3.618 ± 0.029

1.542 ± 0.024

10.131 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.646 ± 0.015

1.689 ± 0.02

6.264 ± 0.033

2.875 ± 0.023

7.769 ± 0.048

5.416 ± 0.028

6.498 ± 0.037

9.396 ± 0.032

1.523 ± 0.016

1.808 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski