Dermacoccus nishinomiyaensis
Average proteome isoelectric point is 6.12
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2255 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A075JFS0|A0A075JFS0_9MICO Uncharacterized protein OS=Dermacoccus nishinomiyaensis OX=1274 GN=HX89_06420 PE=4 SV=1
MM1 pKa = 8.05 SDD3 pKa = 3.27 LQYY6 pKa = 10.81 PADD9 pKa = 3.54 LRR11 pKa = 11.84 YY12 pKa = 8.76 TEE14 pKa = 4.03 DD15 pKa = 3.95 HH16 pKa = 6.54 EE17 pKa = 4.53 WVADD21 pKa = 3.65 KK22 pKa = 11.67 GEE24 pKa = 4.12 GTVRR28 pKa = 11.84 VGITSYY34 pKa = 11.35 AQDD37 pKa = 3.3 ALGDD41 pKa = 3.79 VVYY44 pKa = 11.12 VSLPAVGDD52 pKa = 3.69 TVTAGEE58 pKa = 4.26 SCGEE62 pKa = 4.14 VEE64 pKa = 4.43 STKK67 pKa = 10.7 SVSDD71 pKa = 4.28 LYY73 pKa = 11.54 SPLDD77 pKa = 3.68 GEE79 pKa = 4.41 IVAVNDD85 pKa = 3.98 TLDD88 pKa = 3.97 AEE90 pKa = 4.79 PGTVNTDD97 pKa = 3.24 PYY99 pKa = 9.85 EE100 pKa = 4.51 SGWMFEE106 pKa = 4.08 IRR108 pKa = 11.84 VSDD111 pKa = 4.08 ASAVEE116 pKa = 3.74 ALMDD120 pKa = 3.63 VEE122 pKa = 5.35 AYY124 pKa = 9.33 RR125 pKa = 11.84 ASLDD129 pKa = 3.33
Molecular weight: 13.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.734
IPC2_protein 3.643
IPC_protein 3.63
Toseland 3.414
ProMoST 3.745
Dawson 3.617
Bjellqvist 3.821
Wikipedia 3.567
Rodwell 3.465
Grimsley 3.325
Solomon 3.605
Lehninger 3.567
Nozaki 3.745
DTASelect 3.948
Thurlkill 3.478
EMBOSS 3.567
Sillero 3.745
Patrickios 0.896
IPC_peptide 3.605
IPC2_peptide 3.719
IPC2.peptide.svr19 3.743
Protein with the highest isoelectric point:
>tr|A0A075JC91|A0A075JC91_9MICO Urease subunit alpha OS=Dermacoccus nishinomiyaensis OX=1274 GN=ureC PE=3 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 AKK15 pKa = 8.7 THH17 pKa = 5.15 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 AILANRR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 10.21 GRR40 pKa = 11.84 KK41 pKa = 8.15 QLSAA45 pKa = 3.9
Molecular weight: 5.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.676
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.398
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.215
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2255
0
2255
732796
37
1809
325.0
34.86
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.174 ± 0.073
0.754 ± 0.014
6.589 ± 0.041
5.763 ± 0.048
2.959 ± 0.03
8.925 ± 0.043
2.277 ± 0.023
3.976 ± 0.031
2.691 ± 0.042
9.48 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.174 ± 0.02
2.067 ± 0.026
5.179 ± 0.036
2.793 ± 0.027
7.212 ± 0.047
5.629 ± 0.04
6.094 ± 0.038
8.881 ± 0.05
1.465 ± 0.021
1.92 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here