Callorhinchus milii (Ghost shark)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Chondrichthyes; Holocephali; Chimaeriformes; Callorhinchidae; Callorhinchus

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49245 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4W3K8G4|A0A4W3K8G4_CALMI Reverse transcriptase domain-containing protein OS=Callorhinchus milii OX=7868 PE=4 SV=1
EEE2 pKa = 5.0EE3 pKa = 5.14DDD5 pKa = 3.51DDD7 pKa = 4.11EE8 pKa = 6.04DDD10 pKa = 3.54EE11 pKa = 4.64EE12 pKa = 4.25EE13 pKa = 5.55DD14 pKa = 5.23DDD16 pKa = 4.8EE17 pKa = 4.53EE18 pKa = 6.18DD19 pKa = 6.15DD20 pKa = 5.41DDD22 pKa = 4.46EE23 pKa = 4.8EE24 pKa = 6.25DD25 pKa = 5.69DD26 pKa = 3.91DDD28 pKa = 3.96EE29 pKa = 4.47GEEE32 pKa = 4.1EE33 pKa = 4.11EE34 pKa = 5.26DD35 pKa = 6.35EEE37 pKa = 6.29DD38 pKa = 4.16EE39 pKa = 5.56DD40 pKa = 4.41EE41 pKa = 5.56DD42 pKa = 4.39EE43 pKa = 4.35AEEE46 pKa = 5.0DD47 pKa = 3.54TVFNLIPSVKKK58 pKa = 9.83RR59 pKa = 11.84ASSDDD64 pKa = 3.26EE65 pKa = 4.24DD66 pKa = 4.12EEE68 pKa = 4.42CPICLNTFRR77 pKa = 11.84DDD79 pKa = 3.83AIGTPEEE86 pKa = 3.76CAHHH90 pKa = 6.02YY91 pKa = 10.35CLDDD95 pKa = 5.18ILEEE99 pKa = 4.15AKKK102 pKa = 10.66ANSCPVDDD110 pKa = 4.37IIFNHHH116 pKa = 5.36CVRR119 pKa = 11.84THHH122 pKa = 6.89GGKKK126 pKa = 8.31LKKK129 pKa = 9.5KK130 pKa = 10.36PVQNPDDD137 pKa = 2.94KK138 pKa = 11.08NEEE141 pKa = 3.97EE142 pKa = 4.14EEE144 pKa = 4.05EE145 pKa = 3.97DD146 pKa = 3.67TNCEEE151 pKa = 3.71CGRR154 pKa = 11.84SDDD157 pKa = 4.01EEE159 pKa = 4.35DD160 pKa = 3.69LLLCDDD166 pKa = 3.78CDDD169 pKa = 2.92GYYY172 pKa = 10.95HH173 pKa = 5.22EEE175 pKa = 4.79LDDD178 pKa = 4.35PLHHH182 pKa = 5.47VPVEEE187 pKa = 3.63EE188 pKa = 4.7FCPEEE193 pKa = 4.06AINNPTDDD201 pKa = 3.61E

Molecular weight: 22.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4W3IZ16|A0A4W3IZ16_CALMI ShKT domain-containing protein OS=Callorhinchus milii OX=7868 PE=3 SV=1
MM1 pKa = 7.36SPSPGLAPPSDD12 pKa = 3.96RR13 pKa = 11.84GLSPPLRR20 pKa = 11.84GVPGLAPPLRR30 pKa = 11.84GVPGLAPPLRR40 pKa = 11.84GVPGLAPPLRR50 pKa = 11.84GVPGLAPPLRR60 pKa = 11.84GVPGLAPPLRR70 pKa = 11.84GVPGLAPPLRR80 pKa = 11.84GVPGLAPPLRR90 pKa = 11.84GVPGLVPPLRR100 pKa = 11.84GVPGLAPPLRR110 pKa = 11.84GVPGLAPPLRR120 pKa = 11.84GVPGLAPPLRR130 pKa = 11.84GVPGLAPPLRR140 pKa = 11.84GVPGLAPPLRR150 pKa = 11.84GVPGLAPPLRR160 pKa = 11.84GVPGLAPPLRR170 pKa = 11.84GVPGLAPPLRR180 pKa = 11.84GVPGLAPPLRR190 pKa = 11.84GVPGLAPPLRR200 pKa = 11.84ATGLHH205 pKa = 5.81RR206 pKa = 11.84VLNN209 pKa = 4.33

Molecular weight: 20.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

19408

29837

49245

31969766

9

8624

649.2

72.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.181 ± 0.008

2.289 ± 0.01

5.142 ± 0.008

6.951 ± 0.014

3.901 ± 0.007

5.886 ± 0.011

2.597 ± 0.005

5.237 ± 0.007

6.12 ± 0.012

9.791 ± 0.012

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.343 ± 0.005

4.326 ± 0.006

5.059 ± 0.012

4.714 ± 0.01

5.304 ± 0.008

8.06 ± 0.013

5.562 ± 0.007

6.323 ± 0.007

1.203 ± 0.004

2.97 ± 0.006
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski