Loktanella atrilutea
Average proteome isoelectric point is 6.23
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4069 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1M4SMJ2|A0A1M4SMJ2_9RHOB Uncharacterized protein OS=Loktanella atrilutea OX=366533 GN=SAMN05444339_10177 PE=4 SV=1
MM1 pKa = 7.33 RR2 pKa = 11.84 QDD4 pKa = 4.11 LRR6 pKa = 11.84 ITLALIGMTSPAFGDD21 pKa = 4.32 PYY23 pKa = 11.15 DD24 pKa = 3.64 GTYY27 pKa = 10.22 RR28 pKa = 11.84 QAANSEE34 pKa = 4.26 CALVGIDD41 pKa = 3.54 GGSVRR46 pKa = 11.84 IADD49 pKa = 4.37 GIFYY53 pKa = 10.21 GVEE56 pKa = 4.01 VSCRR60 pKa = 11.84 MTNPVNVLDD69 pKa = 4.47 MDD71 pKa = 4.37 ALLYY75 pKa = 10.39 TMQCSGEE82 pKa = 4.0 DD83 pKa = 3.32 QEE85 pKa = 4.58 FSEE88 pKa = 5.69 RR89 pKa = 11.84 AMLMNKK95 pKa = 9.68 AQGDD99 pKa = 4.54 GIIMVWDD106 pKa = 5.08 GYY108 pKa = 11.46 AFVYY112 pKa = 10.07 DD113 pKa = 4.21 RR114 pKa = 11.84 CPEE117 pKa = 4.28 PGKK120 pKa = 9.83 ATVPGPVADD129 pKa = 4.82 AAAPDD134 pKa = 4.71 DD135 pKa = 4.33 GAGDD139 pKa = 4.32 AVPGIADD146 pKa = 3.65 VAPEE150 pKa = 4.14 VTPAPASVPTAPAAGDD166 pKa = 3.42
Molecular weight: 17.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.724
IPC2_protein 3.795
IPC_protein 3.795
Toseland 3.567
ProMoST 3.923
Dawson 3.795
Bjellqvist 3.999
Wikipedia 3.757
Rodwell 3.617
Grimsley 3.478
Solomon 3.783
Lehninger 3.745
Nozaki 3.923
DTASelect 4.19
Thurlkill 3.63
EMBOSS 3.77
Sillero 3.923
Patrickios 1.901
IPC_peptide 3.783
IPC2_peptide 3.897
IPC2.peptide.svr19 3.847
Protein with the highest isoelectric point:
>tr|A0A1M5EG49|A0A1M5EG49_9RHOB Transposase OS=Loktanella atrilutea OX=366533 GN=SAMN05444339_11276 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNRR10 pKa = 11.84 VRR12 pKa = 11.84 KK13 pKa = 8.99 NRR15 pKa = 11.84 HH16 pKa = 3.77 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATPSGRR28 pKa = 11.84 KK29 pKa = 8.93 IINARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.31 GRR39 pKa = 11.84 AKK41 pKa = 10.69 LSAA44 pKa = 3.92
Molecular weight: 5.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.199
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.647
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.466
Sillero 12.954
Patrickios 12.369
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.154
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4069
0
4069
1257508
27
2833
309.0
33.36
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.933 ± 0.061
0.861 ± 0.012
6.688 ± 0.04
4.784 ± 0.038
3.537 ± 0.025
8.755 ± 0.046
2.047 ± 0.022
5.113 ± 0.028
2.735 ± 0.035
9.949 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.831 ± 0.023
2.505 ± 0.024
5.221 ± 0.032
3.253 ± 0.02
6.858 ± 0.039
4.801 ± 0.024
6.16 ± 0.032
7.443 ± 0.032
1.374 ± 0.017
2.151 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here