Marinobacter vulgaris
Average proteome isoelectric point is 6.0
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3437 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2V3ZLS3|A0A2V3ZLS3_9ALTE PilZ domain-containing protein OS=Marinobacter vulgaris OX=1928331 GN=DIT71_08395 PE=4 SV=1
MM1 pKa = 7.95 RR2 pKa = 11.84 KK3 pKa = 9.69 PIALMLGLASVSALAVSQTATADD26 pKa = 3.2 IYY28 pKa = 11.31 KK29 pKa = 10.48 SGGGSLYY36 pKa = 10.98 AGLNYY41 pKa = 10.53 SFIDD45 pKa = 3.57 IEE47 pKa = 4.32 SGRR50 pKa = 11.84 DD51 pKa = 3.65 DD52 pKa = 4.14 VDD54 pKa = 3.69 VGALSAKK61 pKa = 10.3 VGGLVSPYY69 pKa = 10.19 FGLEE73 pKa = 3.39 ARR75 pKa = 11.84 AGFGVDD81 pKa = 4.17 DD82 pKa = 4.35 DD83 pKa = 4.98 QIDD86 pKa = 4.07 GVDD89 pKa = 3.51 YY90 pKa = 11.42 SLDD93 pKa = 3.63 NFFGGYY99 pKa = 7.76 ATLNLANEE107 pKa = 4.63 SPATPYY113 pKa = 10.84 LIFGFTRR120 pKa = 11.84 IEE122 pKa = 4.1 LEE124 pKa = 4.01 AQSALGTTTEE134 pKa = 4.52 DD135 pKa = 3.36 EE136 pKa = 4.37 TDD138 pKa = 3.77 FSYY141 pKa = 11.1 GAGVNVDD148 pKa = 3.39 ITPEE152 pKa = 3.82 VAGNLEE158 pKa = 3.67 YY159 pKa = 10.08 MRR161 pKa = 11.84 YY162 pKa = 8.55 YY163 pKa = 10.54 DD164 pKa = 4.41 KK165 pKa = 11.51 SGATVDD171 pKa = 3.78 GLGLGVTFSFF181 pKa = 4.98
Molecular weight: 19.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.705
IPC2_protein 3.808
IPC_protein 3.795
Toseland 3.579
ProMoST 3.973
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.745
Rodwell 3.63
Grimsley 3.49
Solomon 3.783
Lehninger 3.745
Nozaki 3.91
DTASelect 4.151
Thurlkill 3.643
EMBOSS 3.745
Sillero 3.923
Patrickios 0.998
IPC_peptide 3.783
IPC2_peptide 3.897
IPC2.peptide.svr19 3.817
Protein with the highest isoelectric point:
>tr|A0A2V4A429|A0A2V4A429_9ALTE Nitrate reductase molybdenum cofactor assembly chaperone OS=Marinobacter vulgaris OX=1928331 GN=narJ PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.34 RR12 pKa = 11.84 KK13 pKa = 9.1 RR14 pKa = 11.84 VHH16 pKa = 6.26 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATANGRR28 pKa = 11.84 KK29 pKa = 9.34 VISRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.41 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LSAA44 pKa = 3.91
Molecular weight: 5.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3437
0
3437
1140680
21
2489
331.9
36.62
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.784 ± 0.041
0.926 ± 0.014
5.904 ± 0.035
6.648 ± 0.038
3.8 ± 0.03
7.909 ± 0.032
2.218 ± 0.022
5.347 ± 0.033
3.558 ± 0.04
10.587 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.624 ± 0.02
3.195 ± 0.021
4.705 ± 0.026
3.86 ± 0.025
6.623 ± 0.04
5.946 ± 0.03
5.135 ± 0.024
7.333 ± 0.03
1.379 ± 0.017
2.52 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here