Gordonia aichiensis NBRC 108223
Average proteome isoelectric point is 5.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4613 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|L7KSF6|L7KSF6_9ACTN Uncharacterized protein OS=Gordonia aichiensis NBRC 108223 OX=1220583 GN=GOACH_33_00130 PE=4 SV=1
MM1 pKa = 7.4 SADD4 pKa = 4.21 DD5 pKa = 5.55 DD6 pKa = 5.18 SILTALGISPDD17 pKa = 3.97 DD18 pKa = 3.82 VQAPPAGVFEE28 pKa = 4.84 HH29 pKa = 6.77 ALSDD33 pKa = 3.84 AFASDD38 pKa = 3.94 APADD42 pKa = 4.18 DD43 pKa = 3.7 STVPVMDD50 pKa = 5.68 DD51 pKa = 3.5 EE52 pKa = 4.77 PAVPDD57 pKa = 4.48 DD58 pKa = 5.02 DD59 pKa = 5.78 LVVDD63 pKa = 5.08 DD64 pKa = 5.34 GVHH67 pKa = 6.75 HH68 pKa = 6.76 EE69 pKa = 5.01 GPDD72 pKa = 3.33 EE73 pKa = 4.19 GHH75 pKa = 7.56 DD76 pKa = 3.74 GGTDD80 pKa = 3.26 HH81 pKa = 7.66 DD82 pKa = 4.94 GDD84 pKa = 5.71 AIPADD89 pKa = 3.62 PAVLHH94 pKa = 6.71 GNDD97 pKa = 5.06 DD98 pKa = 3.43 PTLQHH103 pKa = 7.14 DD104 pKa = 4.2 EE105 pKa = 4.08 ASGGHH110 pKa = 5.6 EE111 pKa = 4.49 VDD113 pKa = 5.77 LADD116 pKa = 5.16 HH117 pKa = 7.79 DD118 pKa = 4.96 YY119 pKa = 11.55 GDD121 pKa = 4.08 HH122 pKa = 7.27 DD123 pKa = 4.49 GSGFHH128 pKa = 7.72 DD129 pKa = 4.12 GGDD132 pKa = 3.52 YY133 pKa = 10.99 HH134 pKa = 7.63 LL135 pKa = 5.39
Molecular weight: 13.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.698
IPC2_protein 3.554
IPC_protein 3.605
Toseland 3.363
ProMoST 3.808
Dawson 3.63
Bjellqvist 3.783
Wikipedia 3.617
Rodwell 3.427
Grimsley 3.274
Solomon 3.617
Lehninger 3.579
Nozaki 3.745
DTASelect 4.062
Thurlkill 3.439
EMBOSS 3.617
Sillero 3.732
Patrickios 1.1
IPC_peptide 3.605
IPC2_peptide 3.706
IPC2.peptide.svr19 3.706
Protein with the highest isoelectric point:
>tr|L7KM77|L7KM77_9ACTN Mce family protein OS=Gordonia aichiensis NBRC 108223 OX=1220583 GN=mceA PE=4 SV=1
MM1 pKa = 7.69 AKK3 pKa = 10.06 GKK5 pKa = 8.69 RR6 pKa = 11.84 TFQPNNRR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 ARR17 pKa = 11.84 VHH19 pKa = 5.99 GFRR22 pKa = 11.84 LRR24 pKa = 11.84 MRR26 pKa = 11.84 TRR28 pKa = 11.84 AGRR31 pKa = 11.84 SIINTRR37 pKa = 11.84 RR38 pKa = 11.84 SKK40 pKa = 10.92 GRR42 pKa = 11.84 AKK44 pKa = 10.73 LSAA47 pKa = 3.92
Molecular weight: 5.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.527
IPC2_protein 11.316
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.705
Grimsley 13.13
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.427
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.238
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4613
0
4613
1538364
44
10958
333.5
35.8
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.521 ± 0.048
0.765 ± 0.01
6.896 ± 0.031
5.208 ± 0.032
2.985 ± 0.02
8.721 ± 0.031
2.217 ± 0.016
4.562 ± 0.023
2.144 ± 0.032
9.39 ± 0.04
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.992 ± 0.014
2.17 ± 0.019
5.441 ± 0.029
2.758 ± 0.017
7.328 ± 0.034
6.108 ± 0.026
6.428 ± 0.026
8.85 ± 0.036
1.428 ± 0.015
2.087 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here