Moniliophthora roreri
Average proteome isoelectric point is 6.43
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 19789 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0W0FA27|A0A0W0FA27_9AGAR Zn(2)-C6 fungal-type domain-containing protein (Fragment) OS=Moniliophthora roreri OX=221103 GN=WG66_14199 PE=4 SV=1
MM1 pKa = 6.98 NHH3 pKa = 6.72 HH4 pKa = 7.28 LEE6 pKa = 4.29 TMSPYY11 pKa = 10.47 SPCEE15 pKa = 3.36 EE16 pKa = 5.37 DD17 pKa = 4.29 EE18 pKa = 4.08 IQEE21 pKa = 4.16 ILSQFYY27 pKa = 9.13 LTDD30 pKa = 3.37 ATEE33 pKa = 4.81 SEE35 pKa = 4.42 DD36 pKa = 4.89 TDD38 pKa = 4.82 LCDD41 pKa = 5.91 LDD43 pKa = 4.82 IEE45 pKa = 4.62 SSLYY49 pKa = 10.58 RR50 pKa = 11.84 SDD52 pKa = 4.65 EE53 pKa = 4.02 EE54 pKa = 4.38 EE55 pKa = 3.99 EE56 pKa = 5.18 DD57 pKa = 3.44 IEE59 pKa = 5.48 LYY61 pKa = 10.46 EE62 pKa = 5.55 LYY64 pKa = 10.95 GDD66 pKa = 5.87 DD67 pKa = 6.37 DD68 pKa = 6.78 DD69 pKa = 7.76 DD70 pKa = 7.75 DD71 pKa = 7.19 DD72 pKa = 7.69 DD73 pKa = 7.66 DD74 pKa = 7.39 DD75 pKa = 6.41 DD76 pKa = 4.73 DD77 pKa = 4.93 HH78 pKa = 8.39 DD79 pKa = 4.95 RR80 pKa = 11.84 YY81 pKa = 10.87 SYY83 pKa = 11.14 SSRR86 pKa = 11.84 EE87 pKa = 3.93 SDD89 pKa = 4.63 EE90 pKa = 5.15 SDD92 pKa = 3.74 LDD94 pKa = 3.98 YY95 pKa = 11.26 PPDD98 pKa = 4.65 PPTEE102 pKa = 4.06 NYY104 pKa = 10.32 LPSFQDD110 pKa = 3.05 SCQDD114 pKa = 3.06 GEE116 pKa = 4.57 LRR118 pKa = 11.84 SDD120 pKa = 4.81 PIDD123 pKa = 3.78 SFHH126 pKa = 7.7 DD127 pKa = 3.25 ATYY130 pKa = 10.68 RR131 pKa = 11.84 NSKK134 pKa = 9.5 MSDD137 pKa = 2.83 VSLTIDD143 pKa = 3.26 GLGSRR148 pKa = 11.84 EE149 pKa = 3.89 EE150 pKa = 4.15 PSRR153 pKa = 11.84 GRR155 pKa = 11.84 PLQKK159 pKa = 10.99 KK160 pKa = 8.78 MIGWWASASPGHH172 pKa = 6.23 EE173 pKa = 4.46 LYY175 pKa = 10.64 QQVASRR181 pKa = 11.84 HH182 pKa = 5.52 DD183 pKa = 3.83 SEE185 pKa = 4.73 SS186 pKa = 3.11
Molecular weight: 21.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.687
IPC2_protein 3.719
IPC_protein 3.745
Toseland 3.528
ProMoST 3.91
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.668
Rodwell 3.567
Grimsley 3.427
Solomon 3.732
Lehninger 3.694
Nozaki 3.846
DTASelect 4.088
Thurlkill 3.579
EMBOSS 3.681
Sillero 3.872
Patrickios 1.252
IPC_peptide 3.732
IPC2_peptide 3.846
IPC2.peptide.svr19 3.762
Protein with the highest isoelectric point:
>tr|A0A0W0FB63|A0A0W0FB63_9AGAR Uncharacterized protein OS=Moniliophthora roreri OX=221103 GN=WG66_14044 PE=4 SV=1
GG1 pKa = 6.66 GGARR5 pKa = 11.84 PFVSGGGRR13 pKa = 11.84 RR14 pKa = 11.84 LVNGGTSLPAGGGGGGGGIFWRR36 pKa = 11.84 RR37 pKa = 3.02
Molecular weight: 3.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.387
IPC2_protein 10.818
IPC_protein 12.442
Toseland 12.603
ProMoST 13.115
Dawson 12.603
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.106
Grimsley 12.647
Solomon 13.1
Lehninger 12.998
Nozaki 12.603
DTASelect 12.603
Thurlkill 12.603
EMBOSS 13.1
Sillero 12.603
Patrickios 11.901
IPC_peptide 13.1
IPC2_peptide 12.091
IPC2.peptide.svr19 9.169
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
19789
0
19789
7744644
8
4998
391.4
43.51
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.621 ± 0.016
1.241 ± 0.006
5.596 ± 0.011
6.057 ± 0.018
3.95 ± 0.01
6.274 ± 0.015
2.517 ± 0.008
5.251 ± 0.011
4.88 ± 0.017
9.281 ± 0.018
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.164 ± 0.007
3.803 ± 0.011
6.326 ± 0.021
3.826 ± 0.011
5.779 ± 0.013
8.779 ± 0.02
6.126 ± 0.011
6.212 ± 0.014
1.513 ± 0.007
2.805 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here