Scleroderma citrinum Foug A
Average proteome isoelectric point is 6.75
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 20903 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0C3CS27|A0A0C3CS27_9AGAM Uncharacterized protein (Fragment) OS=Scleroderma citrinum Foug A OX=1036808 GN=SCLCIDRAFT_88157 PE=4 SV=1
MM1 pKa = 7.88 GIGNPRR7 pKa = 11.84 DD8 pKa = 3.42 EE9 pKa = 4.68 YY10 pKa = 10.69 YY11 pKa = 11.38 SNGEE15 pKa = 3.91 GLLPPEE21 pKa = 4.63 PVSPQTPPLYY31 pKa = 10.38 NSPCIQDD38 pKa = 3.23 EE39 pKa = 4.54 DD40 pKa = 3.98 GDD42 pKa = 4.22 ARR44 pKa = 11.84 STPADD49 pKa = 3.56 YY50 pKa = 10.89 QEE52 pKa = 4.05 EE53 pKa = 4.24 HH54 pKa = 6.51 SAEE57 pKa = 4.08 SSTNFNKK64 pKa = 10.14 IYY66 pKa = 10.46 RR67 pKa = 11.84 LYY69 pKa = 10.6 INDD72 pKa = 4.89 LGDD75 pKa = 4.79 HH76 pKa = 7.16 DD77 pKa = 6.13 LLDD80 pKa = 4.8 DD81 pKa = 5.44 DD82 pKa = 5.34 EE83 pKa = 5.98 DD84 pKa = 4.13 AAEE87 pKa = 4.47 LKK89 pKa = 11.13 SMADD93 pKa = 3.63 LLHH96 pKa = 7.34 DD97 pKa = 3.85 IVV99 pKa = 4.39
Molecular weight: 11.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.711
IPC2_protein 3.808
IPC_protein 3.795
Toseland 3.579
ProMoST 3.961
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.732
Rodwell 3.617
Grimsley 3.49
Solomon 3.77
Lehninger 3.732
Nozaki 3.91
DTASelect 4.139
Thurlkill 3.643
EMBOSS 3.732
Sillero 3.91
Patrickios 0.947
IPC_peptide 3.77
IPC2_peptide 3.884
IPC2.peptide.svr19 3.81
Protein with the highest isoelectric point:
>tr|A0A0C2ZX09|A0A0C2ZX09_9AGAM Uncharacterized protein OS=Scleroderma citrinum Foug A OX=1036808 GN=SCLCIDRAFT_193335 PE=4 SV=1
WW1 pKa = 7.59 RR2 pKa = 11.84 ISKK5 pKa = 10.4 GGWRR9 pKa = 11.84 ISKK12 pKa = 8.82 VVQRR16 pKa = 11.84 ISMVVWRR23 pKa = 11.84 ISMVRR28 pKa = 11.84 WRR30 pKa = 11.84 ISKK33 pKa = 9.18 GVWRR37 pKa = 11.84 IFKK40 pKa = 9.81 GVWRR44 pKa = 11.84 IFKK47 pKa = 9.83 GVWRR51 pKa = 11.84 ISKK54 pKa = 7.73 MVWRR58 pKa = 11.84 ISKK61 pKa = 10.26 RR62 pKa = 3.34
Molecular weight: 7.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.522
IPC2_protein 11.257
IPC_protein 12.837
Toseland 13.013
ProMoST 13.51
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.735
Grimsley 13.056
Solomon 13.51
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.457
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.175
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
20898
5
20903
6241616
49
5050
298.6
33.22
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.829 ± 0.016
1.768 ± 0.009
5.549 ± 0.013
5.69 ± 0.016
3.72 ± 0.014
6.168 ± 0.018
3.04 ± 0.011
5.007 ± 0.015
4.477 ± 0.017
9.395 ± 0.024
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.29 ± 0.008
3.487 ± 0.011
6.277 ± 0.024
3.919 ± 0.013
6.122 ± 0.016
8.594 ± 0.024
6.036 ± 0.013
6.395 ± 0.013
1.571 ± 0.008
2.667 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here