Rice yellow mottle virus
Average proteome isoelectric point is 6.73
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q9DH71|Q9DH71_9VIRU Capsid protein OS=Rice yellow mottle virus OX=31744 GN=CP PE=2 SV=1
MM1 pKa = 7.21 TRR3 pKa = 11.84 LEE5 pKa = 4.02 VLIRR9 pKa = 11.84 PTQQTVAKK17 pKa = 9.05 AIAVGYY23 pKa = 6.42 THH25 pKa = 7.55 ALTWVWHH32 pKa = 4.94 SQTWDD37 pKa = 2.89 VDD39 pKa = 3.92 AVSDD43 pKa = 4.2 PVLSADD49 pKa = 4.14 FNPEE53 pKa = 3.3 KK54 pKa = 10.81 VGWVSVSFACTRR66 pKa = 11.84 CTAHH70 pKa = 6.92 YY71 pKa = 7.3 YY72 pKa = 9.93 TSEE75 pKa = 3.88 QVKK78 pKa = 9.43 YY79 pKa = 9.57 FVNIPPVHH87 pKa = 6.52 YY88 pKa = 10.56 DD89 pKa = 3.55 VVCADD94 pKa = 4.57 CEE96 pKa = 4.13 RR97 pKa = 11.84 SVQQDD102 pKa = 3.48 DD103 pKa = 4.48 EE104 pKa = 4.7 IDD106 pKa = 3.74 RR107 pKa = 11.84 EE108 pKa = 4.06 HH109 pKa = 7.61 DD110 pKa = 3.29 EE111 pKa = 4.3 RR112 pKa = 11.84 NAEE115 pKa = 3.79 ISACNARR122 pKa = 11.84 ALSEE126 pKa = 4.13 GRR128 pKa = 11.84 PASLVYY134 pKa = 10.44 LSRR137 pKa = 11.84 DD138 pKa = 3.07 ACDD141 pKa = 3.44 IPEE144 pKa = 4.24 HH145 pKa = 6.76 SGTCRR150 pKa = 11.84 FDD152 pKa = 3.18 KK153 pKa = 10.97 YY154 pKa = 11.58 LNFF157 pKa = 4.56
Molecular weight: 17.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.728
IPC2_protein 4.94
IPC_protein 4.863
Toseland 4.787
ProMoST 5.016
Dawson 4.863
Bjellqvist 4.991
Wikipedia 4.762
Rodwell 4.762
Grimsley 4.711
Solomon 4.863
Lehninger 4.825
Nozaki 4.991
DTASelect 5.181
Thurlkill 4.813
EMBOSS 4.813
Sillero 5.054
Patrickios 2.854
IPC_peptide 4.876
IPC2_peptide 5.041
IPC2.peptide.svr19 5.005
Protein with the highest isoelectric point:
>tr|Q9DH71|Q9DH71_9VIRU Capsid protein OS=Rice yellow mottle virus OX=31744 GN=CP PE=2 SV=1
MM1 pKa = 7.29 ARR3 pKa = 11.84 KK4 pKa = 9.21 GKK6 pKa = 8.36 KK7 pKa = 7.99 TNSNQGQQGKK17 pKa = 9.92 RR18 pKa = 11.84 KK19 pKa = 8.91 GRR21 pKa = 11.84 RR22 pKa = 11.84 PRR24 pKa = 11.84 GRR26 pKa = 11.84 SAEE29 pKa = 4.1 PQLQRR34 pKa = 11.84 APVAQASRR42 pKa = 11.84 ISGTVPGPLSSNTWPLHH59 pKa = 4.61 SVEE62 pKa = 4.68 FLADD66 pKa = 4.59 FKK68 pKa = 11.12 RR69 pKa = 11.84 SSTSADD75 pKa = 2.85 ATTYY79 pKa = 11.22 DD80 pKa = 4.09 CVPFNLPRR88 pKa = 11.84 VWSLARR94 pKa = 11.84 CYY96 pKa = 11.33 SMWKK100 pKa = 6.65 PTRR103 pKa = 11.84 WDD105 pKa = 3.27 VVYY108 pKa = 10.44 LPEE111 pKa = 4.59 VSATVAGSIEE121 pKa = 4.04 MCFLYY126 pKa = 10.32 DD127 pKa = 3.68 YY128 pKa = 11.33 ADD130 pKa = 4.86 TIPSDD135 pKa = 3.41 TGKK138 pKa = 9.75 MSRR141 pKa = 11.84 TAGFVTSSVWYY152 pKa = 9.41 GAEE155 pKa = 4.21 GCHH158 pKa = 6.29 LLSGGSARR166 pKa = 11.84 NAVVASMDD174 pKa = 3.68 CSRR177 pKa = 11.84 VGWKK181 pKa = 9.93 RR182 pKa = 11.84 VTSSIPSSVDD192 pKa = 3.03 PNVVNTILPARR203 pKa = 11.84 LAVRR207 pKa = 11.84 SSIKK211 pKa = 9.37 PTVSDD216 pKa = 3.84 TPGKK220 pKa = 10.24 LYY222 pKa = 11.0 VIASMVLRR230 pKa = 11.84 DD231 pKa = 4.13 PVDD234 pKa = 3.43 PTLNTT239 pKa = 3.52
Molecular weight: 25.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.229
IPC2_protein 9.238
IPC_protein 9.326
Toseland 9.882
ProMoST 9.633
Dawson 10.116
Bjellqvist 9.853
Wikipedia 10.306
Rodwell 10.409
Grimsley 10.189
Solomon 10.16
Lehninger 10.131
Nozaki 9.984
DTASelect 9.823
Thurlkill 9.97
EMBOSS 10.306
Sillero 10.058
Patrickios 9.721
IPC_peptide 10.16
IPC2_peptide 8.814
IPC2.peptide.svr19 8.085
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1505
157
605
376.3
41.4
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.508 ± 0.504
2.326 ± 0.578
5.05 ± 0.595
5.847 ± 0.984
3.256 ± 0.424
7.375 ± 0.91
2.458 ± 0.431
4.12 ± 0.318
4.186 ± 0.466
7.508 ± 0.789
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.329 ± 0.292
2.99 ± 0.173
6.246 ± 0.502
3.256 ± 0.438
6.91 ± 0.589
11.096 ± 1.381
5.449 ± 0.837
8.173 ± 0.882
2.259 ± 0.576
2.658 ± 0.407
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here