Corynebacterium sp. 2189
Average proteome isoelectric point is 5.8
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2133 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2U1T7W4|A0A2U1T7W4_9CORY Methionine--tRNA ligase OS=Corynebacterium sp. 2189 OX=2173852 GN=metG PE=3 SV=1
MM1 pKa = 6.9 NTNSQPVDD9 pKa = 3.83 DD10 pKa = 6.01 ADD12 pKa = 5.44 LDD14 pKa = 4.61 DD15 pKa = 5.59 DD16 pKa = 4.12 TTQPRR21 pKa = 11.84 RR22 pKa = 11.84 PMLWIVATIAVIAAVGAAFALFQQSSTPTEE52 pKa = 4.07 EE53 pKa = 5.04 AAEE56 pKa = 4.08 QPPATPEE63 pKa = 3.88 NVAEE67 pKa = 4.18 EE68 pKa = 4.76 PEE70 pKa = 4.13 VGPAGGYY77 pKa = 10.11 VDD79 pKa = 4.03 TSAPVEE85 pKa = 4.14 MYY87 pKa = 10.33 IPSLDD92 pKa = 3.26 MHH94 pKa = 7.2 AGFEE98 pKa = 4.36 SGPCRR103 pKa = 11.84 VKK105 pKa = 10.92 DD106 pKa = 3.8 GLIDD110 pKa = 4.09 PVTMDD115 pKa = 4.53 LACAYY120 pKa = 9.42 TSDD123 pKa = 4.32 DD124 pKa = 4.11 KK125 pKa = 11.34 PYY127 pKa = 10.85 SLPGSDD133 pKa = 4.62 AEE135 pKa = 5.21 DD136 pKa = 3.24 IVVLAGHH143 pKa = 6.93 TGAGVPGVFDD153 pKa = 4.8 DD154 pKa = 6.32 LYY156 pKa = 11.42 DD157 pKa = 4.41 GDD159 pKa = 4.77 ADD161 pKa = 3.72 EE162 pKa = 4.91 HH163 pKa = 5.48 TVEE166 pKa = 5.42 IGDD169 pKa = 3.84 EE170 pKa = 4.09 LLVRR174 pKa = 11.84 TVTSGDD180 pKa = 2.63 QWLSYY185 pKa = 10.37 RR186 pKa = 11.84 ATDD189 pKa = 3.21 LHH191 pKa = 6.15 TPQKK195 pKa = 10.5 EE196 pKa = 4.03 VLAQSTEE203 pKa = 3.63 IWGSEE208 pKa = 3.85 PTPGRR213 pKa = 11.84 LLTISCVQPLNPFAPSLEE231 pKa = 4.06 NAVVGWEE238 pKa = 3.96 YY239 pKa = 11.53 AGLVDD244 pKa = 4.91 VAAA247 pKa = 5.05
Molecular weight: 26.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.736
IPC2_protein 3.719
IPC_protein 3.732
Toseland 3.516
ProMoST 3.884
Dawson 3.719
Bjellqvist 3.872
Wikipedia 3.643
Rodwell 3.554
Grimsley 3.414
Solomon 3.706
Lehninger 3.668
Nozaki 3.834
DTASelect 4.05
Thurlkill 3.567
EMBOSS 3.656
Sillero 3.846
Patrickios 1.85
IPC_peptide 3.706
IPC2_peptide 3.821
IPC2.peptide.svr19 3.768
Protein with the highest isoelectric point:
>tr|A0A2U1T9S0|A0A2U1T9S0_9CORY Uncharacterized protein OS=Corynebacterium sp. 2189 OX=2173852 GN=DF222_01395 PE=4 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.38 KK7 pKa = 8.42 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.06 RR11 pKa = 11.84 MSKK14 pKa = 9.63 KK15 pKa = 9.39 KK16 pKa = 9.04 HH17 pKa = 5.39 RR18 pKa = 11.84 KK19 pKa = 5.37 MLRR22 pKa = 11.84 RR23 pKa = 11.84 TRR25 pKa = 11.84 VQRR28 pKa = 11.84 RR29 pKa = 11.84 KK30 pKa = 10.11 LGKK33 pKa = 9.87
Molecular weight: 4.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.507
IPC2_protein 11.184
IPC_protein 12.749
Toseland 12.925
ProMoST 13.408
Dawson 12.925
Bjellqvist 12.91
Wikipedia 13.393
Rodwell 12.735
Grimsley 12.954
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.457
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.111
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2133
0
2133
688899
31
2998
323.0
35.04
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.762 ± 0.07
0.636 ± 0.013
6.392 ± 0.045
6.792 ± 0.055
3.204 ± 0.034
8.425 ± 0.046
2.294 ± 0.026
5.099 ± 0.038
2.788 ± 0.04
9.653 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.12 ± 0.021
2.664 ± 0.033
5.018 ± 0.039
3.08 ± 0.028
6.715 ± 0.057
5.505 ± 0.037
6.093 ± 0.032
8.157 ± 0.044
1.333 ± 0.021
2.271 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here