Aedes albopictus (Asian tiger mosquito) (Stegomyia albopicta)
Average proteome isoelectric point is 6.7
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 16616 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A023ES83|A0A023ES83_AEDAL RNA helicase OS=Aedes albopictus OX=7160 GN=109433584 PE=2 SV=1
MM1 pKa = 7.7 KK2 pKa = 10.14 MPDD5 pKa = 3.5 HH6 pKa = 7.08 NIGCSSSNNGYY17 pKa = 9.94 SGNYY21 pKa = 8.55 SDD23 pKa = 6.07 NYY25 pKa = 11.16 DD26 pKa = 4.1 SDD28 pKa = 4.28 PDD30 pKa = 4.53 GYY32 pKa = 9.32 ITPPNEE38 pKa = 4.16 DD39 pKa = 3.39 NSNGGGPNLDD49 pKa = 3.67 EE50 pKa = 5.73 DD51 pKa = 4.41 FSEE54 pKa = 4.46 YY55 pKa = 8.62 MWMEE59 pKa = 3.9 NEE61 pKa = 4.27 EE62 pKa = 4.34 EE63 pKa = 3.94 FDD65 pKa = 3.57 KK66 pKa = 11.72 QEE68 pKa = 3.89 MQRR71 pKa = 11.84 LEE73 pKa = 4.25 EE74 pKa = 4.1 EE75 pKa = 4.48 ALMEE79 pKa = 4.0 QCIEE83 pKa = 4.84 AMLQDD88 pKa = 4.51 EE89 pKa = 5.42 LDD91 pKa = 3.68 AAQNPSGDD99 pKa = 3.58 PTLEE103 pKa = 3.66 VDD105 pKa = 4.18 SPKK108 pKa = 10.73 DD109 pKa = 3.25 LCHH112 pKa = 6.75 ALSSLQVQSKK122 pKa = 6.21 EE123 pKa = 3.62 QKK125 pKa = 10.31 VPVEE129 pKa = 3.92 QSTLNPLAAEE139 pKa = 4.46 FVPSVLSTTSVV150 pKa = 2.79
Molecular weight: 16.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.736
IPC2_protein 3.706
IPC_protein 3.668
Toseland 3.465
ProMoST 3.821
Dawson 3.643
Bjellqvist 3.795
Wikipedia 3.554
Rodwell 3.503
Grimsley 3.389
Solomon 3.63
Lehninger 3.579
Nozaki 3.757
DTASelect 3.935
Thurlkill 3.516
EMBOSS 3.567
Sillero 3.783
Patrickios 0.947
IPC_peptide 3.63
IPC2_peptide 3.757
IPC2.peptide.svr19 3.709
Protein with the highest isoelectric point:
>tr|A0A182GBB5|A0A182GBB5_AEDAL Uncharacterized protein OS=Aedes albopictus OX=7160 GN=RP20_CCG006852 PE=4 SV=1
MM1 pKa = 7.42 SAHH4 pKa = 5.4 KK5 pKa = 9.38 TFRR8 pKa = 11.84 IKK10 pKa = 10.56 QKK12 pKa = 10.1 LAKK15 pKa = 9.71 KK16 pKa = 10.08 LKK18 pKa = 8.58 QNRR21 pKa = 11.84 PIPQWIRR28 pKa = 11.84 MRR30 pKa = 11.84 TGNTIRR36 pKa = 11.84 YY37 pKa = 5.79 NAKK40 pKa = 8.47 RR41 pKa = 11.84 RR42 pKa = 11.84 HH43 pKa = 4.1 WRR45 pKa = 11.84 RR46 pKa = 11.84 TKK48 pKa = 10.89 LKK50 pKa = 10.55 LL51 pKa = 3.39
Molecular weight: 6.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.393
IPC2_protein 11.067
IPC_protein 12.398
Toseland 12.559
ProMoST 13.042
Dawson 12.574
Bjellqvist 12.559
Wikipedia 13.027
Rodwell 12.398
Grimsley 12.603
Solomon 13.056
Lehninger 12.954
Nozaki 12.559
DTASelect 12.559
Thurlkill 12.559
EMBOSS 13.056
Sillero 12.559
Patrickios 12.135
IPC_peptide 13.056
IPC2_peptide 12.032
IPC2.peptide.svr19 8.988
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
16616
0
16616
7624980
49
10820
458.9
51.51
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.671 ± 0.016
1.948 ± 0.014
5.577 ± 0.014
6.624 ± 0.022
3.83 ± 0.016
5.82 ± 0.02
2.59 ± 0.011
5.311 ± 0.015
6.179 ± 0.022
8.828 ± 0.025
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.395 ± 0.009
4.789 ± 0.014
5.235 ± 0.026
4.47 ± 0.023
5.494 ± 0.019
8.077 ± 0.026
5.699 ± 0.018
6.313 ± 0.015
1.033 ± 0.007
3.115 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here