Mycobacterium bohemicum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Mycobacteriaceae; Mycobacterium

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4677 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X1R9T1|A0A1X1R9T1_9MYCO Nitroreductase OS=Mycobacterium bohemicum OX=56425 GN=AWB93_06985 PE=3 SV=1
MM1 pKa = 7.66SYY3 pKa = 10.82LVALPEE9 pKa = 4.28FLNSAAADD17 pKa = 3.69LAGIGSGLSAANAAAALPTTAVVAAAEE44 pKa = 4.6DD45 pKa = 4.09EE46 pKa = 4.53VSAAIASLFSGRR58 pKa = 11.84GQAFQALSAQAAAFHH73 pKa = 5.68TQFVQALGGAEE84 pKa = 3.99SAYY87 pKa = 9.49TATEE91 pKa = 3.94AASASPLQTVVQDD104 pKa = 3.89AQSLTVFSPVKK115 pKa = 10.43DD116 pKa = 3.43LTGRR120 pKa = 11.84PLVGDD125 pKa = 4.43GANGTAGSGQAGGDD139 pKa = 3.64GGWIYY144 pKa = 11.3GNGGNGGSGAPGQAGGAGGSAFLFGNGGQGGAGGVGTTGATGNAATSTNGVGGGNGGVGGVGGNGGLLLGNGGVGGIGGAGGQGGAGGPGTAGFVNGTGGAQGGNGGNGGAGGHH258 pKa = 6.44AEE260 pKa = 4.16GLAALLGGHH269 pKa = 7.28AGANGNGGGGGNGGAGGQGGAGFTGTGATGGNGGGGGTGGAGGLGGSAGGTGAIAGAAGNGGAGGNGGGGGDD341 pKa = 5.66DD342 pKa = 3.27IDD344 pKa = 3.81QAVGGGFTAGNGGNGGNGGTGITGGNGGDD373 pKa = 3.61GGAGGSALAGYY384 pKa = 8.17TGGAYY389 pKa = 10.03AVGGGGGGGGAGGNSTGGGTGGTGGHH415 pKa = 6.49GGDD418 pKa = 3.64GGEE421 pKa = 4.26AQSSGGGTVVEE432 pKa = 4.78GNGGDD437 pKa = 3.49GGGGGGAAGIAGGPGGAGGGGGAVNNGSVTGIQNGGDD474 pKa = 3.57GGNGAGTGGGLHH486 pKa = 6.01SVGGEE491 pKa = 3.64VDD493 pKa = 3.59EE494 pKa = 4.79AAGGAVAAGGGGGGGGGLTVGTAGSGGNGGSASITGAGTGVGGEE538 pKa = 4.32GGAPDD543 pKa = 4.71SVGQPGLTSASGGTPTAGHH562 pKa = 6.73GGGSQGQGGGGGGGGAATDD581 pKa = 4.16FAGSAAGGNGGQGGG595 pKa = 3.59

Molecular weight: 49.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X1R115|A0A1X1R115_9MYCO DUF732 domain-containing protein OS=Mycobacterium bohemicum OX=56425 GN=AWB93_16750 PE=4 SV=1
MM1 pKa = 7.25SVALGLMARR10 pKa = 11.84FLARR14 pKa = 11.84AVPALAVTAPGLMARR29 pKa = 11.84FLARR33 pKa = 11.84AVPALAVTAPGLMARR48 pKa = 11.84FLARR52 pKa = 11.84AVPALAVTALGLMARR67 pKa = 11.84FLTRR71 pKa = 11.84AVPALAVTALGSVPP85 pKa = 3.66

Molecular weight: 8.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4677

0

4677

1557375

29

4197

333.0

35.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.365 ± 0.053

0.84 ± 0.01

6.146 ± 0.033

5.148 ± 0.036

3.044 ± 0.024

9.328 ± 0.111

2.233 ± 0.017

4.052 ± 0.025

2.066 ± 0.022

9.829 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.031 ± 0.018

2.251 ± 0.031

6.031 ± 0.03

2.918 ± 0.018

7.511 ± 0.045

5.265 ± 0.023

5.707 ± 0.026

8.579 ± 0.035

1.501 ± 0.016

2.157 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski