Polaribacter sp. Hel1_85
Average proteome isoelectric point is 6.94
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3382 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A099XNY3|A0A099XNY3_9FLAO Cell division trigger factor OS=Polaribacter sp. Hel1_85 OX=1250005 GN=PHEL85_2926 PE=4 SV=1
MM1 pKa = 7.21 PTGSFTFADD10 pKa = 3.83 PNGTSNEE17 pKa = 3.89 KK18 pKa = 10.44 QIEE21 pKa = 4.29 NNHH24 pKa = 5.85 YY25 pKa = 10.67 AVIDD29 pKa = 3.65 PTHH32 pKa = 6.35 IQDD35 pKa = 4.63 AYY37 pKa = 10.06 PSDD40 pKa = 5.52 GYY42 pKa = 10.14 WFWSGNIPPGVSSGVNSPDD61 pKa = 3.45 TDD63 pKa = 3.36 DD64 pKa = 4.25 HH65 pKa = 5.8 TTGDD69 pKa = 3.21 INGAVMAINAGTTLDD84 pKa = 3.66 YY85 pKa = 11.03 LYY87 pKa = 11.08 VRR89 pKa = 11.84 SVSLNAGDD97 pKa = 5.12 CYY99 pKa = 10.68 RR100 pKa = 11.84 ISYY103 pKa = 8.68 WIYY106 pKa = 10.69 LVNNDD111 pKa = 3.46 AQIEE115 pKa = 4.21 VDD117 pKa = 5.8 LRR119 pKa = 11.84 DD120 pKa = 3.48 TSNQSVLNNDD130 pKa = 3.06 NDD132 pKa = 3.89 YY133 pKa = 9.21 QTNLITIIDD142 pKa = 3.46 NWAQYY147 pKa = 10.99 SIDD150 pKa = 3.52 FKK152 pKa = 11.74 VPNTCTNTDD161 pKa = 3.88 FEE163 pKa = 4.74 VTLRR167 pKa = 11.84 NDD169 pKa = 3.24 YY170 pKa = 10.34 PLNSGNDD177 pKa = 3.62 YY178 pKa = 11.04 YY179 pKa = 11.71 VDD181 pKa = 4.8 DD182 pKa = 4.46 IFMEE186 pKa = 4.35 KK187 pKa = 10.54 LSTCTATEE195 pKa = 4.05 VANCPLGVSQTDD207 pKa = 3.22 KK208 pKa = 11.37 DD209 pKa = 3.89 GDD211 pKa = 4.71 SIPDD215 pKa = 3.5 MDD217 pKa = 7.2 DD218 pKa = 3.68 IDD220 pKa = 5.47 DD221 pKa = 4.99 DD222 pKa = 4.49 NDD224 pKa = 4.89 GILDD228 pKa = 3.82 TLEE231 pKa = 4.75 SNGNNPDD238 pKa = 3.3 GDD240 pKa = 3.99 EE241 pKa = 5.09 DD242 pKa = 5.11 GDD244 pKa = 4.4 GIPNFKK250 pKa = 10.92 DD251 pKa = 3.29 NTDD254 pKa = 3.27 GTLGNGSPGTDD265 pKa = 3.07 YY266 pKa = 11.47 TDD268 pKa = 3.62 TNNDD272 pKa = 4.62 GIPDD276 pKa = 3.82 VYY278 pKa = 11.36 DD279 pKa = 4.16 FDD281 pKa = 7.03 DD282 pKa = 5.42 DD283 pKa = 5.38 GISNHH288 pKa = 6.82 LDD290 pKa = 3.11 LDD292 pKa = 4.06 ADD294 pKa = 3.61 NDD296 pKa = 4.66 GIYY299 pKa = 10.35 DD300 pKa = 3.49 ILEE303 pKa = 4.37 AGGTDD308 pKa = 3.52 SNNNGVADD316 pKa = 4.81 DD317 pKa = 5.45 LSDD320 pKa = 4.26 LDD322 pKa = 4.15 SDD324 pKa = 4.33 GLVDD328 pKa = 5.88 IYY330 pKa = 11.35 DD331 pKa = 4.21 DD332 pKa = 4.03 TCQSPAIPASNYY344 pKa = 9.23 YY345 pKa = 9.08 ATDD348 pKa = 3.34 VTASQHH354 pKa = 6.33 FNNTNNALGAPSNTFSSGPTGWPGSNPFYY383 pKa = 10.92 VVLDD387 pKa = 3.71 FTEE390 pKa = 4.24 TLTVGTEE397 pKa = 3.98 LTIHH401 pKa = 7.01 LGTNTGTASVRR412 pKa = 11.84 VRR414 pKa = 11.84 QTDD417 pKa = 2.99 ASGTGDD423 pKa = 3.68 TNYY426 pKa = 10.22 VAYY429 pKa = 10.25 NVTGTDD435 pKa = 3.49 PTTFTYY441 pKa = 10.13 TITYY445 pKa = 10.56 SNTDD449 pKa = 3.52 YY450 pKa = 11.31 IKK452 pKa = 10.59 IQTYY456 pKa = 9.83 DD457 pKa = 3.38 YY458 pKa = 9.96 NVRR461 pKa = 11.84 VYY463 pKa = 10.46 GVEE466 pKa = 3.95 YY467 pKa = 8.86 GTAASGGEE475 pKa = 4.07 DD476 pKa = 3.47 CAGSALSPIEE486 pKa = 4.28 TTLGTADD493 pKa = 4.53 FLNLDD498 pKa = 4.2 SDD500 pKa = 4.17 GDD502 pKa = 3.98 GCFDD506 pKa = 3.42 VLEE509 pKa = 4.98 AGFTDD514 pKa = 3.8 ANIDD518 pKa = 3.71 GEE520 pKa = 4.37 IDD522 pKa = 3.38 GTSYY526 pKa = 9.05 STKK529 pKa = 10.47 GIVIGSDD536 pKa = 3.28 GYY538 pKa = 9.65 TGTTSAVTDD547 pKa = 3.73 NSNSAACQSLIDD559 pKa = 4.23 SDD561 pKa = 4.67 NDD563 pKa = 3.39 GSTDD567 pKa = 5.51 LDD569 pKa = 5.49 DD570 pKa = 6.77 LDD572 pKa = 5.83 DD573 pKa = 5.79 DD574 pKa = 4.9 NDD576 pKa = 5.08 GILDD580 pKa = 4.12 TIEE583 pKa = 4.53 NNQCGIGGNIVTTIFTEE600 pKa = 4.77 DD601 pKa = 3.73 FGTQNTSNGTKK612 pKa = 10.39 SNTSEE617 pKa = 3.92 YY618 pKa = 10.51 TNYY621 pKa = 10.53 NYY623 pKa = 11.06 YY624 pKa = 9.44 EE625 pKa = 4.57 AEE627 pKa = 4.33 VGTVPTDD634 pKa = 2.88 WMDD637 pKa = 3.41 GSSAPQSLQDD647 pKa = 3.29 GRR649 pKa = 11.84 YY650 pKa = 8.22 TIFNDD655 pKa = 3.19 IQYY658 pKa = 7.4 TASWAATDD666 pKa = 3.3 WQTIGDD672 pKa = 4.34 HH673 pKa = 5.32 TTGSATPSSGRR684 pKa = 11.84 MAIFNANEE692 pKa = 4.14 NIAGLEE698 pKa = 4.23 FYY700 pKa = 10.84 RR701 pKa = 11.84 RR702 pKa = 11.84 TLKK705 pKa = 11.01 NIVAGAPINASYY717 pKa = 9.74 WAMNIYY723 pKa = 6.9 TTPGGSSVIKK733 pKa = 10.3 PNITVQFIQRR743 pKa = 11.84 GNEE746 pKa = 3.82 VYY748 pKa = 10.71 SFNSGDD754 pKa = 3.74 IDD756 pKa = 3.7 PVAAGSTAAWSQFSNQTVFIPTSNDD781 pKa = 3.38 PIEE784 pKa = 5.02 IIFINNTIGGGGNDD798 pKa = 4.32 LAIDD802 pKa = 5.38 DD803 pKa = 4.35 ILITQSFCDD812 pKa = 3.95 EE813 pKa = 5.17 DD814 pKa = 5.49 GDD816 pKa = 5.23 GIPSYY821 pKa = 11.43 LDD823 pKa = 3.83 LDD825 pKa = 4.1 SDD827 pKa = 4.02 NDD829 pKa = 4.67 GIPDD833 pKa = 3.67 VIEE836 pKa = 4.66 AGGTDD841 pKa = 3.55 VNRR844 pKa = 11.84 DD845 pKa = 3.22 GLADD849 pKa = 4.82 DD850 pKa = 6.0 DD851 pKa = 5.42 DD852 pKa = 5.9 NNVNNFATNGIPTTAGTGLTATNTDD877 pKa = 3.51 GDD879 pKa = 4.42 SLPDD883 pKa = 3.76 YY884 pKa = 11.21 LDD886 pKa = 3.77 IDD888 pKa = 4.59 ADD890 pKa = 3.86 NDD892 pKa = 4.72 GIPDD896 pKa = 4.06 NIEE899 pKa = 3.97 AQSSNRR905 pKa = 11.84 YY906 pKa = 8.31 IAPSSVGATIIDD918 pKa = 3.97 TNNNGVDD925 pKa = 3.76 DD926 pKa = 4.49 NYY928 pKa = 10.31 EE929 pKa = 3.91 TGSIVGLNPINTDD942 pKa = 2.98 SSNDD946 pKa = 3.92 TIPDD950 pKa = 3.79 YY951 pKa = 11.4 LDD953 pKa = 3.87 SDD955 pKa = 4.24 SDD957 pKa = 3.77 NDD959 pKa = 4.62 GINDD963 pKa = 3.46 VLEE966 pKa = 4.87 NGDD969 pKa = 3.76 TDD971 pKa = 4.63 NIASGLDD978 pKa = 3.39 TDD980 pKa = 6.1 GDD982 pKa = 4.15 GLDD985 pKa = 4.48 NAFDD989 pKa = 5.15 DD990 pKa = 5.01 NDD992 pKa = 3.91 DD993 pKa = 3.79 SSITGSTINDD1003 pKa = 3.5 GLGIGNKK1010 pKa = 8.05 VTDD1013 pKa = 3.68 SSSLEE1018 pKa = 4.06 SAFGDD1023 pKa = 3.51 EE1024 pKa = 5.99 DD1025 pKa = 5.13 SDD1027 pKa = 4.27 FNPGTGDD1034 pKa = 3.54 LDD1036 pKa = 3.82 YY1037 pKa = 11.0 RR1038 pKa = 11.84 DD1039 pKa = 3.93 SFNPQGTPMITQVYY1053 pKa = 8.87 QFGTEE1058 pKa = 3.45 KK1059 pKa = 10.02 WIEE1062 pKa = 3.75 ITNISTTNSIEE1073 pKa = 3.89 ANLIKK1078 pKa = 10.36 IQLYY1082 pKa = 10.26 LNKK1085 pKa = 10.1 TGDD1088 pKa = 3.76 QTGVTPDD1095 pKa = 3.01 VTYY1098 pKa = 9.98 TVTSALIPGQSIIIGNSANSIINTNAGAITVTNDD1132 pKa = 2.67 ALTNIDD1138 pKa = 4.04 GANDD1142 pKa = 5.59 LITLSTTNDD1151 pKa = 3.06 TTSFANRR1158 pKa = 11.84 YY1159 pKa = 9.15 DD1160 pKa = 3.97 VIEE1163 pKa = 4.35 SSSNKK1168 pKa = 8.88 TSFVRR1173 pKa = 11.84 IDD1175 pKa = 3.32 EE1176 pKa = 4.4 TLTPNSTYY1184 pKa = 10.07 TVSEE1188 pKa = 3.87 WAVFIDD1194 pKa = 5.24 DD1195 pKa = 5.23 ALDD1198 pKa = 4.13 PYY1200 pKa = 11.32 LLLGAGGAEE1209 pKa = 4.04 RR1210 pKa = 11.84 HH1211 pKa = 5.78 PHH1213 pKa = 6.76 DD1214 pKa = 4.65 PLISEE1219 pKa = 4.65 IEE1221 pKa = 4.14 NANAQANIQLGLHH1234 pKa = 6.92 RR1235 pKa = 11.84 IDD1237 pKa = 2.96 ITNRR1241 pKa = 11.84 VAASGGSWDD1250 pKa = 3.43 NGYY1253 pKa = 9.49 PDD1255 pKa = 3.44 RR1256 pKa = 11.84 SRR1258 pKa = 11.84 YY1259 pKa = 9.97 VIIDD1263 pKa = 3.2 EE1264 pKa = 5.21 DD1265 pKa = 4.59 YY1266 pKa = 11.13 NHH1268 pKa = 5.9 TTDD1271 pKa = 3.77 RR1272 pKa = 11.84 LSARR1276 pKa = 11.84 KK1277 pKa = 8.39 LTVNTSKK1284 pKa = 11.03 KK1285 pKa = 10.69 LSITDD1290 pKa = 3.35 NLLAVTNDD1298 pKa = 2.71 ILLNGEE1304 pKa = 3.75 VRR1306 pKa = 11.84 LIGSSQLIQTHH1317 pKa = 5.58 TAASLVTGTGILLVDD1332 pKa = 4.0 QNSTVPSKK1340 pKa = 11.0 YY1341 pKa = 9.12 RR1342 pKa = 11.84 YY1343 pKa = 9.72 NYY1345 pKa = 8.9 MGSPVKK1351 pKa = 10.83 NSTGAANYY1359 pKa = 7.9 TVASIFKK1366 pKa = 10.53 DD1367 pKa = 3.21 GTNPTNHH1374 pKa = 6.59 TGIINTNIAKK1384 pKa = 10.34 DD1385 pKa = 3.41 ITWIGGYY1392 pKa = 10.34 DD1393 pKa = 4.14 GDD1395 pKa = 4.15 TTDD1398 pKa = 5.12 PISLAEE1404 pKa = 3.95 HH1405 pKa = 7.17 WIYY1408 pKa = 10.59 TYY1410 pKa = 11.06 AANGGSRR1417 pKa = 11.84 ASWTQKK1423 pKa = 9.85 FSAGNIPNTDD1433 pKa = 2.71 GFIFKK1438 pKa = 10.89 GPGRR1442 pKa = 11.84 AQNYY1446 pKa = 6.04 TFSGIPKK1453 pKa = 9.8 DD1454 pKa = 3.89 GLITTSIGANEE1465 pKa = 4.57 SYY1467 pKa = 11.06 LVGNPFASAISVKK1480 pKa = 10.4 EE1481 pKa = 4.02 FIEE1484 pKa = 4.2 NNNASISGTLYY1495 pKa = 9.34 FWEE1498 pKa = 4.75 HH1499 pKa = 6.47 ASEE1502 pKa = 4.13 QTTQEE1507 pKa = 4.2 GSSVGHH1513 pKa = 6.24 NFAGYY1518 pKa = 9.31 IGGYY1522 pKa = 6.21 ATRR1525 pKa = 11.84 TIAMGVNAKK1534 pKa = 8.42 TAANTGTVNTTLEE1547 pKa = 4.28 AEE1549 pKa = 4.36 NANTINGTSEE1559 pKa = 4.13 QIVDD1563 pKa = 3.55 NSVTIDD1569 pKa = 3.38 VVRR1572 pKa = 11.84 FNATNNFIEE1581 pKa = 4.74 FEE1583 pKa = 4.41 NIVKK1587 pKa = 10.44 GVDD1590 pKa = 3.28 TLKK1593 pKa = 10.35 IRR1595 pKa = 11.84 YY1596 pKa = 8.69 KK1597 pKa = 11.02 ADD1599 pKa = 3.42 LDD1601 pKa = 3.85 KK1602 pKa = 11.61 NIILKK1607 pKa = 8.3 TQGDD1611 pKa = 4.09 VLKK1614 pKa = 9.49 TINITLPQTSGEE1626 pKa = 3.95 FSIFEE1631 pKa = 4.11 IKK1633 pKa = 10.21 HH1634 pKa = 5.35 CAEE1637 pKa = 4.3 PSDD1640 pKa = 4.58 NITITSNDD1648 pKa = 3.39 TNLLYY1653 pKa = 9.78 IDD1655 pKa = 4.98 HH1656 pKa = 7.25 LNLYY1660 pKa = 10.21 DD1661 pKa = 4.29 GDD1663 pKa = 4.25 GLISCVPNVGGDD1675 pKa = 3.37 QITYY1679 pKa = 8.92 TEE1681 pKa = 4.48 PEE1683 pKa = 4.14 PYY1685 pKa = 9.75 IAIGQGFFVQGDD1697 pKa = 3.93 NVDD1700 pKa = 3.47 GGQIIFNNSQRR1711 pKa = 11.84 EE1712 pKa = 4.13 FKK1714 pKa = 10.68 TEE1716 pKa = 3.96 GTGSSVFLKK1725 pKa = 11.0 SNAKK1729 pKa = 8.41 KK1730 pKa = 7.32 TTDD1733 pKa = 3.31 NNSILTLPVIKK1744 pKa = 10.49 LGMNFNSTDD1753 pKa = 3.58 DD1754 pKa = 3.39 NKK1756 pKa = 10.89 KK1757 pKa = 7.8 YY1758 pKa = 10.3 HH1759 pKa = 5.74 RR1760 pKa = 11.84 QIGISFSNYY1769 pKa = 7.8 TSFSYY1774 pKa = 11.01 DD1775 pKa = 2.66 KK1776 pKa = 11.04 GYY1778 pKa = 10.63 DD1779 pKa = 3.3 AEE1781 pKa = 5.36 IYY1783 pKa = 10.5 DD1784 pKa = 3.83 VGNTDD1789 pKa = 4.12 VYY1791 pKa = 10.99 WKK1793 pKa = 10.77 FPDD1796 pKa = 3.99 DD1797 pKa = 3.42 EE1798 pKa = 5.0 RR1799 pKa = 11.84 KK1800 pKa = 10.01 FIIAGVQNITDD1811 pKa = 3.9 DD1812 pKa = 4.34 LEE1814 pKa = 4.65 VPLEE1818 pKa = 3.84 ITMGYY1823 pKa = 9.53 SGEE1826 pKa = 4.08 IVLKK1830 pKa = 9.97 VDD1832 pKa = 3.2 EE1833 pKa = 4.99 MKK1835 pKa = 10.95 NISNNIYY1842 pKa = 8.86 ITDD1845 pKa = 3.8 KK1846 pKa = 10.7 LTGNSYY1852 pKa = 11.06 DD1853 pKa = 3.4 ILNNKK1858 pKa = 9.14 AMLNLEE1864 pKa = 3.92 QGLYY1868 pKa = 9.14 TDD1870 pKa = 4.25 RR1871 pKa = 11.84 FVLAFALTNALNINDD1886 pKa = 4.73 EE1887 pKa = 4.37 IIKK1890 pKa = 10.35 QYY1892 pKa = 9.2 ATVYY1896 pKa = 9.92 VDD1898 pKa = 3.73 HH1899 pKa = 7.14 NNAHH1903 pKa = 6.69 LVITKK1908 pKa = 9.1 NQNINIKK1915 pKa = 9.72 QVSLYY1920 pKa = 10.6 NILGKK1925 pKa = 10.45 KK1926 pKa = 9.06 VGFWNIKK1933 pKa = 7.54 EE1934 pKa = 4.13 QNQKK1938 pKa = 8.93 TKK1940 pKa = 11.06 LSFNKK1945 pKa = 10.02 RR1946 pKa = 11.84 LATGLYY1952 pKa = 6.96 ITKK1955 pKa = 10.1 INTDD1959 pKa = 3.19 KK1960 pKa = 11.51 GQFSQKK1966 pKa = 10.32 IIIEE1970 pKa = 4.13
Molecular weight: 212.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.68
IPC2_protein 3.757
IPC_protein 3.834
Toseland 3.592
ProMoST 3.999
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.783
Rodwell 3.643
Grimsley 3.49
Solomon 3.834
Lehninger 3.795
Nozaki 3.935
DTASelect 4.24
Thurlkill 3.643
EMBOSS 3.795
Sillero 3.948
Patrickios 1.621
IPC_peptide 3.821
IPC2_peptide 3.923
IPC2.peptide.svr19 3.842
Protein with the highest isoelectric point:
>tr|A0A099Y1P6|A0A099Y1P6_9FLAO Anthranilate synthase component II OS=Polaribacter sp. Hel1_85 OX=1250005 GN=PHEL85_2563 PE=4 SV=1
MM1 pKa = 7.79 IDD3 pKa = 3.6 NIRR6 pKa = 11.84 HH7 pKa = 4.66 FFEE10 pKa = 5.22 RR11 pKa = 11.84 NGFHH15 pKa = 6.25 VSSRR19 pKa = 11.84 LADD22 pKa = 4.89 RR23 pKa = 11.84 IGMRR27 pKa = 11.84 ALNVRR32 pKa = 11.84 LFFIYY37 pKa = 9.38 VTFFTVGLSFGFYY50 pKa = 9.77 LTMAFLFKK58 pKa = 10.7 LKK60 pKa = 11.02 DD61 pKa = 3.45 MVHH64 pKa = 6.46 TSRR67 pKa = 11.84 SSVFDD72 pKa = 3.56 LL73 pKa = 4.79
Molecular weight: 8.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.316
IPC2_protein 9.663
IPC_protein 10.599
Toseland 10.482
ProMoST 10.277
Dawson 10.643
Bjellqvist 10.379
Wikipedia 10.877
Rodwell 10.745
Grimsley 10.716
Solomon 10.76
Lehninger 10.73
Nozaki 10.452
DTASelect 10.394
Thurlkill 10.511
EMBOSS 10.891
Sillero 10.57
Patrickios 10.599
IPC_peptide 10.76
IPC2_peptide 9.282
IPC2.peptide.svr19 8.562
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3382
0
3382
1146162
37
3283
338.9
38.31
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.793 ± 0.037
0.692 ± 0.013
5.472 ± 0.045
6.578 ± 0.038
5.483 ± 0.036
6.257 ± 0.048
1.633 ± 0.021
8.491 ± 0.042
8.781 ± 0.065
9.044 ± 0.05
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.99 ± 0.024
6.802 ± 0.052
3.113 ± 0.026
3.043 ± 0.019
3.007 ± 0.028
6.768 ± 0.038
5.965 ± 0.049
6.021 ± 0.029
1.037 ± 0.016
4.031 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here