Amino acid dipepetide frequency for Polaribacter sp. Hel1_85

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	3.754AlaAla: 3.754 ± 0.078	0.473AlaCys: 0.473 ± 0.022	3.122AlaAsp: 3.122 ± 0.063	3.518AlaGlu: 3.518 ± 0.065	3.136AlaPhe: 3.136 ± 0.06	3.765AlaGly: 3.765 ± 0.078	0.954AlaHis: 0.954 ± 0.028	5.248AlaIle: 5.248 ± 0.072	4.673AlaLys: 4.673 ± 0.077	5.313AlaLeu: 5.313 ± 0.072	1.229AlaMet: 1.229 ± 0.038	3.404AlaAsn: 3.404 ± 0.061	1.667AlaPro: 1.667 ± 0.041	1.895AlaGln: 1.895 ± 0.041	1.71AlaArg: 1.71 ± 0.042	4.051AlaSer: 4.051 ± 0.068	3.707AlaThr: 3.707 ± 0.077	3.596AlaVal: 3.596 ± 0.062	0.579AlaTrp: 0.579 ± 0.021	2.131AlaTyr: 2.131 ± 0.041	0.0AlaXaa: 0.0 ± 0.0
Cys	0.431CysAla: 0.431 ± 0.02	0.081CysCys: 0.081 ± 0.009	0.382CysAsp: 0.382 ± 0.021	0.418CysGlu: 0.418 ± 0.021	0.396CysPhe: 0.396 ± 0.021	0.563CysGly: 0.563 ± 0.026	0.174CysHis: 0.174 ± 0.019	0.542CysIle: 0.542 ± 0.023	0.557CysLys: 0.557 ± 0.024	0.571CysLeu: 0.571 ± 0.024	0.133CysMet: 0.133 ± 0.011	0.431CysAsn: 0.431 ± 0.02	0.266CysPro: 0.266 ± 0.016	0.182CysGln: 0.182 ± 0.012	0.156CysArg: 0.156 ± 0.011	0.518CysSer: 0.518 ± 0.023	0.391CysThr: 0.391 ± 0.021	0.397CysVal: 0.397 ± 0.02	0.066CysTrp: 0.066 ± 0.009	0.269CysTyr: 0.269 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	3.553AspAla: 3.553 ± 0.057	0.404AspCys: 0.404 ± 0.019	2.984AspAsp: 2.984 ± 0.082	3.643AspGlu: 3.643 ± 0.06	3.824AspPhe: 3.824 ± 0.057	3.767AspGly: 3.767 ± 0.111	0.694AspHis: 0.694 ± 0.028	4.562AspIle: 4.562 ± 0.069	4.597AspLys: 4.597 ± 0.08	5.093AspLeu: 5.093 ± 0.078	0.889AspMet: 0.889 ± 0.035	3.511AspAsn: 3.511 ± 0.077	1.348AspPro: 1.348 ± 0.039	1.166AspGln: 1.166 ± 0.035	1.559AspArg: 1.559 ± 0.037	3.214AspSer: 3.214 ± 0.071	2.708AspThr: 2.708 ± 0.065	3.774AspVal: 3.774 ± 0.066	0.734AspTrp: 0.734 ± 0.028	2.695AspTyr: 2.695 ± 0.056	0.0AspXaa: 0.0 ± 0.0
Glu	3.844GluAla: 3.844 ± 0.064	0.328GluCys: 0.328 ± 0.019	3.419GluAsp: 3.419 ± 0.052	4.842GluGlu: 4.842 ± 0.084	3.154GluPhe: 3.154 ± 0.055	3.496GluGly: 3.496 ± 0.065	1.004GluHis: 1.004 ± 0.034	6.101GluIle: 6.101 ± 0.088	6.595GluLys: 6.595 ± 0.103	6.043GluLeu: 6.043 ± 0.073	1.499GluMet: 1.499 ± 0.04	5.542GluAsn: 5.542 ± 0.081	1.329GluPro: 1.329 ± 0.034	1.853GluGln: 1.853 ± 0.04	2.195GluArg: 2.195 ± 0.05	3.267GluSer: 3.267 ± 0.053	3.856GluThr: 3.856 ± 0.062	4.374GluVal: 4.374 ± 0.064	0.587GluTrp: 0.587 ± 0.024	2.452GluTyr: 2.452 ± 0.049	0.0GluXaa: 0.0 ± 0.0
Phe	2.822PheAla: 2.822 ± 0.058	0.4PheCys: 0.4 ± 0.019	3.311PheAsp: 3.311 ± 0.061	3.322PheGlu: 3.322 ± 0.055	2.957PhePhe: 2.957 ± 0.068	3.753PheGly: 3.753 ± 0.074	0.798PheHis: 0.798 ± 0.024	4.437PheIle: 4.437 ± 0.075	4.34PheLys: 4.34 ± 0.07	5.073PheLeu: 5.073 ± 0.086	1.129PheMet: 1.129 ± 0.033	3.719PheAsn: 3.719 ± 0.075	1.645PhePro: 1.645 ± 0.04	1.468PheGln: 1.468 ± 0.037	1.452PheArg: 1.452 ± 0.038	4.623PheSer: 4.623 ± 0.081	3.429PheThr: 3.429 ± 0.061	3.111PheVal: 3.111 ± 0.058	0.618PheTrp: 0.618 ± 0.024	2.419PheTyr: 2.419 ± 0.055	0.0PheXaa: 0.0 ± 0.0
Gly	3.853GlyAla: 3.853 ± 0.079	0.507GlyCys: 0.507 ± 0.022	3.349GlyAsp: 3.349 ± 0.07	3.433GlyGlu: 3.433 ± 0.053	3.926GlyPhe: 3.926 ± 0.066	4.426GlyGly: 4.426 ± 0.095	1.03GlyHis: 1.03 ± 0.03	5.587GlyIle: 5.587 ± 0.079	5.228GlyLys: 5.228 ± 0.078	5.345GlyLeu: 5.345 ± 0.069	1.433GlyMet: 1.433 ± 0.035	3.935GlyAsn: 3.935 ± 0.085	1.17GlyPro: 1.17 ± 0.038	1.616GlyGln: 1.616 ± 0.041	1.875GlyArg: 1.875 ± 0.047	4.056GlySer: 4.056 ± 0.082	4.07GlyThr: 4.07 ± 0.103	4.304GlyVal: 4.304 ± 0.08	0.715GlyTrp: 0.715 ± 0.028	2.713GlyTyr: 2.713 ± 0.056	0.0GlyXaa: 0.0 ± 0.0
His	0.798HisAla: 0.798 ± 0.027	0.14HisCys: 0.14 ± 0.011	0.737HisAsp: 0.737 ± 0.026	0.811HisGlu: 0.811 ± 0.027	1.078HisPhe: 1.078 ± 0.03	0.944HisGly: 0.944 ± 0.027	0.462HisHis: 0.462 ± 0.025	1.318HisIle: 1.318 ± 0.036	1.325HisLys: 1.325 ± 0.033	1.688HisLeu: 1.688 ± 0.047	0.303HisMet: 0.303 ± 0.016	0.983HisAsn: 0.983 ± 0.032	0.791HisPro: 0.791 ± 0.032	0.726HisGln: 0.726 ± 0.025	0.599HisArg: 0.599 ± 0.022	0.995HisSer: 0.995 ± 0.031	0.922HisThr: 0.922 ± 0.033	0.803HisVal: 0.803 ± 0.024	0.192HisTrp: 0.192 ± 0.013	0.714HisTyr: 0.714 ± 0.023	0.0HisXaa: 0.0 ± 0.0
Ile	5.347IleAla: 5.347 ± 0.075	0.599IleCys: 0.599 ± 0.023	5.292IleAsp: 5.292 ± 0.088	5.789IleGlu: 5.789 ± 0.086	4.062IlePhe: 4.062 ± 0.065	5.251IleGly: 5.251 ± 0.08	1.393IleHis: 1.393 ± 0.037	7.105IleIle: 7.105 ± 0.1	7.108IleLys: 7.108 ± 0.095	7.365IleLeu: 7.365 ± 0.101	1.305IleMet: 1.305 ± 0.029	5.532IleAsn: 5.532 ± 0.075	3.319IlePro: 3.319 ± 0.06	2.551IleGln: 2.551 ± 0.058	2.415IleArg: 2.415 ± 0.043	6.54IleSer: 6.54 ± 0.083	5.378IleThr: 5.378 ± 0.091	4.762IleVal: 4.762 ± 0.065	0.697IleTrp: 0.697 ± 0.027	3.103IleTyr: 3.103 ± 0.059	0.0IleXaa: 0.0 ± 0.0
Lys	4.767LysAla: 4.767 ± 0.089	0.372LysCys: 0.372 ± 0.022	4.7LysAsp: 4.7 ± 0.072	7.57LysGlu: 7.57 ± 0.114	3.326LysPhe: 3.326 ± 0.053	5.065LysGly: 5.065 ± 0.076	1.515LysHis: 1.515 ± 0.039	7.487LysIle: 7.487 ± 0.098	9.097LysLys: 9.097 ± 0.144	7.009LysLeu: 7.009 ± 0.087	2.234LysMet: 2.234 ± 0.053	6.611LysAsn: 6.611 ± 0.111	2.501LysPro: 2.501 ± 0.059	2.8LysGln: 2.8 ± 0.057	2.887LysArg: 2.887 ± 0.046	5.142LysSer: 5.142 ± 0.08	5.202LysThr: 5.202 ± 0.081	5.203LysVal: 5.203 ± 0.069	0.877LysTrp: 0.877 ± 0.028	3.449LysTyr: 3.449 ± 0.065	0.0LysXaa: 0.0 ± 0.0
Leu	5.188LeuAla: 5.188 ± 0.074	0.567LeuCys: 0.567 ± 0.022	5.002LeuAsp: 5.002 ± 0.079	6.305LeuGlu: 6.305 ± 0.078	5.078LeuPhe: 5.078 ± 0.098	5.712LeuGly: 5.712 ± 0.078	1.403LeuHis: 1.403 ± 0.038	7.268LeuIle: 7.268 ± 0.101	8.415LeuLys: 8.415 ± 0.104	8.365LeuLeu: 8.365 ± 0.118	1.872LeuMet: 1.872 ± 0.049	5.942LeuAsn: 5.942 ± 0.082	3.194LeuPro: 3.194 ± 0.057	2.913LeuGln: 2.913 ± 0.058	2.647LeuArg: 2.647 ± 0.048	6.523LeuSer: 6.523 ± 0.088	5.12LeuThr: 5.12 ± 0.088	5.066LeuVal: 5.066 ± 0.06	0.765LeuTrp: 0.765 ± 0.029	3.097LeuTyr: 3.097 ± 0.061	0.0LeuXaa: 0.0 ± 0.0
Met	1.299MetAla: 1.299 ± 0.039	0.133MetCys: 0.133 ± 0.012	0.988MetAsp: 0.988 ± 0.03	1.134MetGlu: 1.134 ± 0.035	0.907MetPhe: 0.907 ± 0.029	1.172MetGly: 1.172 ± 0.037	0.369MetHis: 0.369 ± 0.019	1.596MetIle: 1.596 ± 0.034	2.221MetLys: 2.221 ± 0.046	1.776MetLeu: 1.776 ± 0.044	0.541MetMet: 0.541 ± 0.026	1.284MetAsn: 1.284 ± 0.038	0.736MetPro: 0.736 ± 0.025	0.711MetGln: 0.711 ± 0.025	0.673MetArg: 0.673 ± 0.023	1.379MetSer: 1.379 ± 0.031	0.967MetThr: 0.967 ± 0.034	1.187MetVal: 1.187 ± 0.035	0.164MetTrp: 0.164 ± 0.011	0.66MetTyr: 0.66 ± 0.021	0.0MetXaa: 0.0 ± 0.0
Asn	3.734AsnAla: 3.734 ± 0.052	0.494AsnCys: 0.494 ± 0.027	3.486AsnAsp: 3.486 ± 0.073	3.877AsnGlu: 3.877 ± 0.061	3.551AsnPhe: 3.551 ± 0.064	4.261AsnGly: 4.261 ± 0.093	1.119AsnHis: 1.119 ± 0.03	5.676AsnIle: 5.676 ± 0.096	5.436AsnLys: 5.436 ± 0.085	6.211AsnLeu: 6.211 ± 0.091	1.197AsnMet: 1.197 ± 0.032	4.911AsnAsn: 4.911 ± 0.117	2.882AsnPro: 2.882 ± 0.06	2.306AsnGln: 2.306 ± 0.047	2.056AsnArg: 2.056 ± 0.045	4.724AsnSer: 4.724 ± 0.084	4.211AsnThr: 4.211 ± 0.088	3.756AsnVal: 3.756 ± 0.069	0.888AsnTrp: 0.888 ± 0.029	3.241AsnTyr: 3.241 ± 0.069	0.0AsnXaa: 0.0 ± 0.0
Pro	1.666ProAla: 1.666 ± 0.041	0.198ProCys: 0.198 ± 0.013	1.671ProAsp: 1.671 ± 0.038	2.439ProGlu: 2.439 ± 0.053	1.892ProPhe: 1.892 ± 0.043	1.61ProGly: 1.61 ± 0.041	0.58ProHis: 0.58 ± 0.024	2.734ProIle: 2.734 ± 0.05	2.785ProLys: 2.785 ± 0.051	2.642ProLeu: 2.642 ± 0.05	0.551ProMet: 0.551 ± 0.023	2.275ProAsn: 2.275 ± 0.045	0.66ProPro: 0.66 ± 0.028	0.966ProGln: 0.966 ± 0.028	0.802ProArg: 0.802 ± 0.03	2.073ProSer: 2.073 ± 0.046	2.119ProThr: 2.119 ± 0.05	1.919ProVal: 1.919 ± 0.041	0.334ProTrp: 0.334 ± 0.02	1.21ProTyr: 1.21 ± 0.037	0.0ProXaa: 0.0 ± 0.0
Gln	1.504GlnAla: 1.504 ± 0.04	0.153GlnCys: 0.153 ± 0.013	1.416GlnAsp: 1.416 ± 0.034	2.187GlnGlu: 2.187 ± 0.043	1.697GlnPhe: 1.697 ± 0.041	1.591GlnGly: 1.591 ± 0.038	0.498GlnHis: 0.498 ± 0.021	2.658GlnIle: 2.658 ± 0.047	2.906GlnLys: 2.906 ± 0.064	3.102GlnLeu: 3.102 ± 0.056	0.668GlnMet: 0.668 ± 0.026	2.121GlnAsn: 2.121 ± 0.044	0.941GlnPro: 0.941 ± 0.027	1.276GlnGln: 1.276 ± 0.039	1.039GlnArg: 1.039 ± 0.029	1.737GlnSer: 1.737 ± 0.036	1.808GlnThr: 1.808 ± 0.033	1.694GlnVal: 1.694 ± 0.038	0.277GlnTrp: 0.277 ± 0.015	1.153GlnTyr: 1.153 ± 0.035	0.0GlnXaa: 0.0 ± 0.0
Arg	1.755ArgAla: 1.755 ± 0.048	0.177ArgCys: 0.177 ± 0.013	1.495ArgAsp: 1.495 ± 0.033	1.91ArgGlu: 1.91 ± 0.043	1.762ArgPhe: 1.762 ± 0.039	1.745ArgGly: 1.745 ± 0.04	0.485ArgHis: 0.485 ± 0.02	2.689ArgIle: 2.689 ± 0.052	2.845ArgLys: 2.845 ± 0.058	2.799ArgLeu: 2.799 ± 0.057	0.689ArgMet: 0.689 ± 0.023	2.015ArgAsn: 2.015 ± 0.04	0.922ArgPro: 0.922 ± 0.03	0.872ArgGln: 0.872 ± 0.027	1.107ArgArg: 1.107 ± 0.039	1.654ArgSer: 1.654 ± 0.044	1.746ArgThr: 1.746 ± 0.035	1.85ArgVal: 1.85 ± 0.043	0.314ArgTrp: 0.314 ± 0.014	1.242ArgTyr: 1.242 ± 0.032	0.0ArgXaa: 0.0 ± 0.0
Ser	3.491SerAla: 3.491 ± 0.06	0.647SerCys: 0.647 ± 0.025	3.632SerAsp: 3.632 ± 0.064	4.354SerGlu: 4.354 ± 0.067	4.211SerPhe: 4.211 ± 0.062	4.663SerGly: 4.663 ± 0.09	1.003SerHis: 1.003 ± 0.035	5.844SerIle: 5.844 ± 0.069	5.916SerLys: 5.916 ± 0.08	6.226SerLeu: 6.226 ± 0.094	1.248SerMet: 1.248 ± 0.033	4.413SerAsn: 4.413 ± 0.089	1.96SerPro: 1.96 ± 0.046	1.963SerGln: 1.963 ± 0.042	1.82SerArg: 1.82 ± 0.045	4.857SerSer: 4.857 ± 0.098	3.76SerThr: 3.76 ± 0.072	4.065SerVal: 4.065 ± 0.065	0.749SerTrp: 0.749 ± 0.03	2.856SerTyr: 2.856 ± 0.052	0.0SerXaa: 0.0 ± 0.0
Thr	3.53ThrAla: 3.53 ± 0.07	0.392ThrCys: 0.392 ± 0.023	3.408ThrAsp: 3.408 ± 0.082	3.719ThrGlu: 3.719 ± 0.061	3.257ThrPhe: 3.257 ± 0.066	4.007ThrGly: 4.007 ± 0.086	0.954ThrHis: 0.954 ± 0.028	5.562ThrIle: 5.562 ± 0.085	4.526ThrLys: 4.526 ± 0.066	5.159ThrLeu: 5.159 ± 0.08	0.874ThrMet: 0.874 ± 0.027	3.914ThrAsn: 3.914 ± 0.092	2.251ThrPro: 2.251 ± 0.051	1.737ThrGln: 1.737 ± 0.036	1.551ThrArg: 1.551 ± 0.039	4.378ThrSer: 4.378 ± 0.091	3.835ThrThr: 3.835 ± 0.1	3.598ThrVal: 3.598 ± 0.061	0.629ThrTrp: 0.629 ± 0.026	2.365ThrTyr: 2.365 ± 0.055	0.0ThrXaa: 0.0 ± 0.0
Val	3.852ValAla: 3.852 ± 0.069	0.47ValCys: 0.47 ± 0.019	3.602ValAsp: 3.602 ± 0.066	3.752ValGlu: 3.752 ± 0.062	3.459ValPhe: 3.459 ± 0.065	3.681ValGly: 3.681 ± 0.064	0.924ValHis: 0.924 ± 0.03	4.815ValIle: 4.815 ± 0.072	4.765ValLys: 4.765 ± 0.073	5.933ValLeu: 5.933 ± 0.085	1.134ValMet: 1.134 ± 0.031	3.789ValAsn: 3.789 ± 0.058	1.866ValPro: 1.866 ± 0.044	1.532ValGln: 1.532 ± 0.035	1.738ValArg: 1.738 ± 0.043	4.56ValSer: 4.56 ± 0.078	3.493ValThr: 3.493 ± 0.071	3.986ValVal: 3.986 ± 0.065	0.585ValTrp: 0.585 ± 0.022	2.271ValTyr: 2.271 ± 0.047	0.0ValXaa: 0.0 ± 0.0
Trp	0.563TrpAla: 0.563 ± 0.026	0.09TrpCys: 0.09 ± 0.009	0.54TrpAsp: 0.54 ± 0.022	0.616TrpGlu: 0.616 ± 0.024	0.636TrpPhe: 0.636 ± 0.024	0.661TrpGly: 0.661 ± 0.027	0.216TrpHis: 0.216 ± 0.014	0.759TrpIle: 0.759 ± 0.028	0.893TrpLys: 0.893 ± 0.031	0.907TrpLeu: 0.907 ± 0.03	0.292TrpMet: 0.292 ± 0.016	0.753TrpAsn: 0.753 ± 0.026	0.223TrpPro: 0.223 ± 0.016	0.395TrpGln: 0.395 ± 0.018	0.357TrpArg: 0.357 ± 0.018	0.675TrpSer: 0.675 ± 0.025	0.539TrpThr: 0.539 ± 0.023	0.67TrpVal: 0.67 ± 0.029	0.138TrpTrp: 0.138 ± 0.011	0.441TrpTyr: 0.441 ± 0.022	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.175TyrAla: 2.175 ± 0.043	0.299TyrCys: 0.299 ± 0.018	2.183TyrAsp: 2.183 ± 0.052	2.142TyrGlu: 2.142 ± 0.046	2.516TyrPhe: 2.516 ± 0.048	2.437TyrGly: 2.437 ± 0.053	0.752TyrHis: 0.752 ± 0.027	2.925TyrIle: 2.925 ± 0.05	3.583TyrLys: 3.583 ± 0.061	3.824TyrLeu: 3.824 ± 0.058	0.674TyrMet: 0.674 ± 0.024	2.935TyrAsn: 2.935 ± 0.052	1.452TyrPro: 1.452 ± 0.038	1.5TyrGln: 1.5 ± 0.037	1.435TyrArg: 1.435 ± 0.04	2.71TyrSer: 2.71 ± 0.048	2.388TyrThr: 2.388 ± 0.049	2.091TyrVal: 2.091 ± 0.042	0.457TyrTrp: 0.457 ± 0.024	1.832TyrTyr: 1.832 ± 0.049	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3382 proteins (1146162 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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