Sandarakinorhabdus cyanobacteriorum

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingosinicellaceae; Sandarakinorhabdus

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3280 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A255YZX0|A0A255YZX0_9SPHN Histidinol dehydrogenase OS=Sandarakinorhabdus cyanobacteriorum OX=1981098 GN=hisD PE=3 SV=1
MM1 pKa = 7.92RR2 pKa = 11.84STFGSASVRR11 pKa = 11.84LYY13 pKa = 11.16YY14 pKa = 10.51LDD16 pKa = 5.69DD17 pKa = 5.11GDD19 pKa = 5.3PVAQTLFYY27 pKa = 10.99GPLDD31 pKa = 3.72EE32 pKa = 5.8ALALAAAQPEE42 pKa = 4.36AVQAGLWIATDD53 pKa = 3.6NDD55 pKa = 3.1VAAYY59 pKa = 10.59LDD61 pKa = 5.29LIDD64 pKa = 4.36

Molecular weight: 6.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A255YEL1|A0A255YEL1_9SPHN Uncharacterized protein OS=Sandarakinorhabdus cyanobacteriorum OX=1981098 GN=CHU93_11185 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.43LVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.38GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84KK29 pKa = 9.31VIAARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.21KK41 pKa = 9.68LTAA44 pKa = 4.27

Molecular weight: 5.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3280

0

3280

1058910

41

7554

322.8

34.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.819 ± 0.082

0.784 ± 0.014

5.667 ± 0.033

4.674 ± 0.044

3.379 ± 0.025

9.248 ± 0.109

1.979 ± 0.025

4.716 ± 0.028

2.732 ± 0.037

10.391 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.366 ± 0.024

2.569 ± 0.05

5.734 ± 0.048

3.244 ± 0.02

7.284 ± 0.053

4.751 ± 0.046

5.093 ± 0.058

7.095 ± 0.033

1.579 ± 0.022

1.896 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski