Acanthocheilonema viteae (Filarial nematode worm) (Dipetalonema viteae)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Spirurina; Spiruromorpha; Filarioidea; Onchocercidae; Acanthocheilonema

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10007 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A498S4L9|A0A498S4L9_ACAVI Methylenetetrahydrofolate reductase OS=Acanthocheilonema viteae OX=6277 GN=NAV_LOCUS435 PE=3 SV=1
MM1 pKa = 7.76LGLQSSNNRR10 pKa = 11.84EE11 pKa = 3.84EE12 pKa = 5.06PIVKK16 pKa = 9.86SSKK19 pKa = 10.42SALTLQYY26 pKa = 11.16AEE28 pKa = 4.12QCPTCRR34 pKa = 11.84THH36 pKa = 7.14TSPSNIVKK44 pKa = 10.33RR45 pKa = 11.84LYY47 pKa = 10.09FANCDD52 pKa = 3.63DD53 pKa = 3.87KK54 pKa = 11.42TVNDD58 pKa = 3.95VGTSSIAAVTDD69 pKa = 3.87HH70 pKa = 6.59NKK72 pKa = 10.13SASITNEE79 pKa = 3.52NARR82 pKa = 11.84SSQAPEE88 pKa = 4.36NINEE92 pKa = 3.97FDD94 pKa = 4.76GNYY97 pKa = 9.79HH98 pKa = 7.66DD99 pKa = 5.59NGKK102 pKa = 10.16NYY104 pKa = 10.33NNNSGYY110 pKa = 10.92DD111 pKa = 3.25SDD113 pKa = 5.8YY114 pKa = 11.17EE115 pKa = 4.33DD116 pKa = 3.66EE117 pKa = 4.54ANRR120 pKa = 11.84AIDD123 pKa = 4.33GDD125 pKa = 4.12FTDD128 pKa = 5.38SSDD131 pKa = 4.94LNMTPSEE138 pKa = 4.13DD139 pKa = 3.79SEE141 pKa = 4.38FATEE145 pKa = 4.84SEE147 pKa = 4.53DD148 pKa = 3.68KK149 pKa = 11.31NEE151 pKa = 3.78EE152 pKa = 3.94LEE154 pKa = 4.17YY155 pKa = 11.1QEE157 pKa = 5.15TISDD161 pKa = 3.79SDD163 pKa = 3.95VVSVLNAPLSITSSVSLGSFAEE185 pKa = 4.36SFGEE189 pKa = 4.11IDD191 pKa = 3.67DD192 pKa = 4.21TLSVSSLEE200 pKa = 3.92YY201 pKa = 11.11DD202 pKa = 3.09NMEE205 pKa = 4.02ISEE208 pKa = 4.49SLDD211 pKa = 3.25DD212 pKa = 4.98HH213 pKa = 8.36DD214 pKa = 5.85EE215 pKa = 4.12ISNDD219 pKa = 2.83GDD221 pKa = 3.92NIFTSGLLIEE231 pKa = 5.42HH232 pKa = 7.57DD233 pKa = 4.62SNDD236 pKa = 3.75DD237 pKa = 3.84DD238 pKa = 5.62SFSSDD243 pKa = 4.41DD244 pKa = 3.87SDD246 pKa = 4.64NGNSDD251 pKa = 3.67SDD253 pKa = 4.23LYY255 pKa = 11.42NYY257 pKa = 11.05DD258 pKa = 2.98HH259 pKa = 7.17FAPHH263 pKa = 6.58LRR265 pKa = 11.84MFNQFILPYY274 pKa = 9.85PMYY277 pKa = 10.62YY278 pKa = 10.24DD279 pKa = 3.5VRR281 pKa = 11.84FQFLFNN287 pKa = 4.07

Molecular weight: 32.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A498SH36|A0A498SH36_ACAVI Uncharacterized protein OS=Acanthocheilonema viteae OX=6277 GN=NAV_LOCUS4068 PE=4 SV=1
MM1 pKa = 8.08PYY3 pKa = 9.69RR4 pKa = 11.84RR5 pKa = 11.84SRR7 pKa = 11.84SVSRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84RR14 pKa = 11.84SRR16 pKa = 11.84SRR18 pKa = 11.84PSRR21 pKa = 11.84DD22 pKa = 3.43VISLSLVPSRR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84SRR38 pKa = 11.84SLSQSLLGYY47 pKa = 10.34DD48 pKa = 4.32LLRR51 pKa = 11.84SRR53 pKa = 11.84SPRR56 pKa = 11.84RR57 pKa = 11.84RR58 pKa = 11.84SYY60 pKa = 9.22IRR62 pKa = 11.84RR63 pKa = 11.84RR64 pKa = 11.84RR65 pKa = 11.84RR66 pKa = 11.84SSSRR70 pKa = 11.84RR71 pKa = 11.84RR72 pKa = 11.84SRR74 pKa = 11.84SRR76 pKa = 11.84RR77 pKa = 11.84RR78 pKa = 11.84ILPRR82 pKa = 11.84RR83 pKa = 11.84RR84 pKa = 11.84LSRR87 pKa = 11.84PRR89 pKa = 11.84RR90 pKa = 11.84RR91 pKa = 11.84SRR93 pKa = 11.84SGYY96 pKa = 9.73RR97 pKa = 11.84YY98 pKa = 9.73RR99 pKa = 5.31

Molecular weight: 12.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10007

0

10007

4369685

19

7164

436.7

49.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.255 ± 0.021

2.171 ± 0.022

5.531 ± 0.019

6.722 ± 0.025

4.081 ± 0.018

5.154 ± 0.028

2.383 ± 0.011

6.523 ± 0.026

6.172 ± 0.025

9.212 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.487 ± 0.009

5.127 ± 0.018

4.343 ± 0.024

4.167 ± 0.024

5.764 ± 0.023

8.166 ± 0.029

5.57 ± 0.017

5.899 ± 0.019

1.098 ± 0.008

3.151 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski