Listeria phage LP-030-3
Average proteome isoelectric point is 5.98
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 73 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A059T5E0|A0A059T5E0_9CAUD Single-stranded DNA-binding protein OS=Listeria phage LP-030-3 OX=1458852 GN=LP030-3_017 PE=3 SV=1
MM1 pKa = 7.5 ILYY4 pKa = 10.08 GVVTYY9 pKa = 10.98 DD10 pKa = 4.66 EE11 pKa = 4.38 ATEE14 pKa = 4.13 WTTDD18 pKa = 3.33 LLTAKK23 pKa = 10.05 KK24 pKa = 9.11 WVEE27 pKa = 3.45 NAKK30 pKa = 10.12 QVFCDD35 pKa = 4.06 GEE37 pKa = 4.13 VDD39 pKa = 2.87 EE40 pKa = 5.94 DD41 pKa = 5.44 YY42 pKa = 11.07 YY43 pKa = 11.99 VKK45 pKa = 10.75 LIKK48 pKa = 10.44 LDD50 pKa = 3.79 VEE52 pKa = 3.94 AFLYY56 pKa = 10.6 DD57 pKa = 3.73 KK58 pKa = 11.01 YY59 pKa = 11.55 DD60 pKa = 3.66 KK61 pKa = 10.53 EE62 pKa = 4.71 TDD64 pKa = 4.31 LSDD67 pKa = 3.64 QLHH70 pKa = 7.14 DD71 pKa = 3.53 EE72 pKa = 4.48 AEE74 pKa = 4.32 TLKK77 pKa = 10.53 EE78 pKa = 3.95 YY79 pKa = 10.69 HH80 pKa = 6.85 LSLDD84 pKa = 3.61 DD85 pKa = 5.17 DD86 pKa = 3.94 GTYY89 pKa = 9.22 MVKK92 pKa = 10.24 EE93 pKa = 4.11 VAKK96 pKa = 10.76
Molecular weight: 11.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.096
IPC2_protein 4.113
IPC_protein 4.075
Toseland 3.872
ProMoST 4.215
Dawson 4.05
Bjellqvist 4.202
Wikipedia 3.961
Rodwell 3.897
Grimsley 3.783
Solomon 4.037
Lehninger 3.999
Nozaki 4.164
DTASelect 4.368
Thurlkill 3.91
EMBOSS 3.973
Sillero 4.19
Patrickios 3.121
IPC_peptide 4.037
IPC2_peptide 4.164
IPC2.peptide.svr19 4.101
Protein with the highest isoelectric point:
>tr|A0A059T7N2|A0A059T7N2_9CAUD Uncharacterized protein OS=Listeria phage LP-030-3 OX=1458852 GN=LP030-3_010 PE=4 SV=1
MM1 pKa = 7.43 GKK3 pKa = 10.33 YY4 pKa = 8.07 YY5 pKa = 10.08 WHH7 pKa = 6.92 VSRR10 pKa = 11.84 LGGKK14 pKa = 6.75 PTEE17 pKa = 3.88 IRR19 pKa = 11.84 HH20 pKa = 5.44 YY21 pKa = 10.3 NHH23 pKa = 5.92 ITKK26 pKa = 9.45 MYY28 pKa = 10.54 KK29 pKa = 10.34 FILRR33 pKa = 11.84 NPAMFKK39 pKa = 10.89 DD40 pKa = 3.79 KK41 pKa = 10.4 TLTIYY46 pKa = 11.15 DD47 pKa = 3.65 DD48 pKa = 4.08 AKK50 pKa = 11.07 AVTNMTFNEE59 pKa = 3.38 IRR61 pKa = 11.84 YY62 pKa = 8.72 RR63 pKa = 11.84 ASLNLCEE70 pKa = 4.24 TVEE73 pKa = 4.09 RR74 pKa = 11.84 KK75 pKa = 9.52 YY76 pKa = 11.49 VLGLTEE82 pKa = 5.31 RR83 pKa = 11.84 LTKK86 pKa = 8.62 EE87 pKa = 3.79 QKK89 pKa = 10.22 GVRR92 pKa = 11.84 SRR94 pKa = 3.51
Molecular weight: 11.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.197
IPC2_protein 9.516
IPC_protein 9.604
Toseland 10.043
ProMoST 9.867
Dawson 10.262
Bjellqvist 9.955
Wikipedia 10.452
Rodwell 10.672
Grimsley 10.35
Solomon 10.292
Lehninger 10.262
Nozaki 10.028
DTASelect 9.94
Thurlkill 10.101
EMBOSS 10.452
Sillero 10.175
Patrickios 10.248
IPC_peptide 10.292
IPC2_peptide 8.624
IPC2.peptide.svr19 8.504
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
73
0
73
12603
41
1788
172.6
19.45
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.871 ± 0.865
0.659 ± 0.131
5.729 ± 0.324
7.95 ± 0.597
4.047 ± 0.23
5.57 ± 0.423
1.293 ± 0.18
7.07 ± 0.24
8.99 ± 0.365
8.157 ± 0.301
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.539 ± 0.141
6.34 ± 0.202
2.388 ± 0.189
3.618 ± 0.218
3.491 ± 0.387
6.633 ± 0.429
6.237 ± 0.202
6.435 ± 0.236
0.952 ± 0.116
4.031 ± 0.324
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here