Weissella viridescens (Lactobacillus viridescens)
Average proteome isoelectric point is 6.07
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1462 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0R2H1H9|A0A0R2H1H9_WEIVI Cobyrinic acid ac-diamide synthase OS=Weissella viridescens OX=1629 GN=parA PE=4 SV=1
MM1 pKa = 7.54 KK2 pKa = 10.8 YY3 pKa = 9.77 EE4 pKa = 4.22 VPDD7 pKa = 3.86 KK8 pKa = 11.2 QIDD11 pKa = 3.96 PPDD14 pKa = 4.7 DD15 pKa = 4.25 PPCIAEE21 pKa = 4.37 DD22 pKa = 4.03 FEE24 pKa = 4.65 KK25 pKa = 11.06 QEE27 pKa = 4.55 LYY29 pKa = 11.02 AGDD32 pKa = 4.57 TVWQDD37 pKa = 2.73 ADD39 pKa = 3.4 GNYY42 pKa = 9.36 IDD44 pKa = 5.11 EE45 pKa = 4.62 EE46 pKa = 4.48 LVTPYY51 pKa = 10.37 FEE53 pKa = 5.39 RR54 pKa = 11.84 YY55 pKa = 8.16 LRR57 pKa = 11.84 YY58 pKa = 9.31 MGAEE62 pKa = 4.1 LIEE65 pKa = 4.61 LGEE68 pKa = 4.14
Molecular weight: 7.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.748
IPC2_protein 3.757
IPC_protein 3.681
Toseland 3.49
ProMoST 3.821
Dawson 3.668
Bjellqvist 3.897
Wikipedia 3.605
Rodwell 3.528
Grimsley 3.414
Solomon 3.643
Lehninger 3.605
Nozaki 3.795
DTASelect 3.961
Thurlkill 3.554
EMBOSS 3.605
Sillero 3.808
Patrickios 0.769
IPC_peptide 3.643
IPC2_peptide 3.783
IPC2.peptide.svr19 3.773
Protein with the highest isoelectric point:
>tr|A0A0R2H8W6|A0A0R2H8W6_WEIVI Amino acid transporter OS=Weissella viridescens OX=1629 GN=IV50_GL001041 PE=4 SV=1
MM1 pKa = 7.36 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 9.69 QPKK8 pKa = 8.59 KK9 pKa = 7.38 RR10 pKa = 11.84 HH11 pKa = 5.81 RR12 pKa = 11.84 SRR14 pKa = 11.84 VHH16 pKa = 6.12 GFRR19 pKa = 11.84 KK20 pKa = 10.01 RR21 pKa = 11.84 MSTSNGRR28 pKa = 11.84 KK29 pKa = 8.01 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 QKK37 pKa = 10.5 GRR39 pKa = 11.84 KK40 pKa = 8.71 ALSAA44 pKa = 3.99
Molecular weight: 5.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.433
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.398
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.135
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.06
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1462
0
1462
449040
44
3356
307.1
34.07
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.956 ± 0.075
0.225 ± 0.013
6.368 ± 0.071
5.935 ± 0.076
4.081 ± 0.055
6.75 ± 0.068
2.053 ± 0.027
6.573 ± 0.063
5.553 ± 0.067
9.466 ± 0.084
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.947 ± 0.034
4.521 ± 0.044
3.778 ± 0.045
4.951 ± 0.073
4.004 ± 0.051
5.634 ± 0.215
6.311 ± 0.046
7.485 ± 0.051
1.016 ± 0.025
3.346 ± 0.042
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here