Phytophthora parasitica virus
Average proteome isoelectric point is 5.89
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0C5AP07|A0A0C5AP07_9VIRU Isolate 1 ORF3 putative replication protein and putative capsid protein genes OS=Phytophthora parasitica virus OX=1608451 PE=4 SV=1
MM1 pKa = 7.47 AHH3 pKa = 6.02 EE4 pKa = 4.01 QHH6 pKa = 6.57 HH7 pKa = 6.61 NIVTEE12 pKa = 4.07 LVEE15 pKa = 4.22 SFIVEE20 pKa = 4.13 EE21 pKa = 4.26 NKK23 pKa = 10.52 ALHH26 pKa = 6.73 DD27 pKa = 4.12 KK28 pKa = 10.94 LRR30 pKa = 11.84 TALNEE35 pKa = 3.88 AQALRR40 pKa = 11.84 LRR42 pKa = 11.84 LTRR45 pKa = 11.84 LNTLYY50 pKa = 11.52 NMAGGLNGRR59 pKa = 11.84 MQQEE63 pKa = 4.13 LRR65 pKa = 11.84 DD66 pKa = 3.54 LHH68 pKa = 6.82 AAIEE72 pKa = 4.16 MMDD75 pKa = 4.39 LIARR79 pKa = 11.84 EE80 pKa = 3.92 YY81 pKa = 10.64 FINNAEE87 pKa = 3.96 ARR89 pKa = 11.84 ARR91 pKa = 11.84 WAPYY95 pKa = 9.88 CAFEE99 pKa = 5.81 DD100 pKa = 5.06 DD101 pKa = 4.54 EE102 pKa = 4.48 FHH104 pKa = 7.49 HH105 pKa = 7.2 LIAEE109 pKa = 4.42 ANAGFPSDD117 pKa = 3.82 LAIVMEE123 pKa = 4.34 PTQEE127 pKa = 4.78 DD128 pKa = 3.71 EE129 pKa = 4.45 DD130 pKa = 4.99 DD131 pKa = 4.02 RR132 pKa = 11.84 DD133 pKa = 3.84 SVIDD137 pKa = 3.6 ILEE140 pKa = 4.25 LTEE143 pKa = 4.68 KK144 pKa = 10.54 EE145 pKa = 4.17 MRR147 pKa = 11.84 DD148 pKa = 3.76 FMDD151 pKa = 3.13 VV152 pKa = 2.91
Molecular weight: 17.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.597
IPC2_protein 4.546
IPC_protein 4.469
Toseland 4.342
ProMoST 4.584
Dawson 4.431
Bjellqvist 4.571
Wikipedia 4.291
Rodwell 4.329
Grimsley 4.253
Solomon 4.418
Lehninger 4.38
Nozaki 4.533
DTASelect 4.673
Thurlkill 4.342
EMBOSS 4.317
Sillero 4.596
Patrickios 3.897
IPC_peptide 4.431
IPC2_peptide 4.584
IPC2.peptide.svr19 4.516
Protein with the highest isoelectric point:
>tr|A0A0C5ALV9|A0A0C5ALV9_9VIRU Isolate 1 ORF3 putative replication protein and putative capsid protein genes OS=Phytophthora parasitica virus OX=1608451 PE=4 SV=1
MM1 pKa = 7.38 APLVPFHH8 pKa = 6.36 TMDD11 pKa = 3.74 RR12 pKa = 11.84 QWDD15 pKa = 3.43 ARR17 pKa = 11.84 FNVPTDD23 pKa = 3.54 EE24 pKa = 5.04 DD25 pKa = 4.55 LEE27 pKa = 4.84 ALLLNVKK34 pKa = 10.34 SEE36 pKa = 3.79 VDD38 pKa = 3.09 QGKK41 pKa = 8.34 FQYY44 pKa = 10.47 CLVGGVEE51 pKa = 4.06 IGDD54 pKa = 3.45 RR55 pKa = 11.84 PYY57 pKa = 10.14 QTDD60 pKa = 3.57 YY61 pKa = 11.11 LIKK64 pKa = 9.15 HH65 pKa = 4.65 VHH67 pKa = 5.3 VALVYY72 pKa = 10.54 NNRR75 pKa = 11.84 VSKK78 pKa = 11.19 NSVLKK83 pKa = 10.65 NLQIKK88 pKa = 9.0 QGNGYY93 pKa = 9.62 YY94 pKa = 10.34 LVPRR98 pKa = 11.84 NRR100 pKa = 11.84 DD101 pKa = 3.12 LPIQGWIDD109 pKa = 3.12 HH110 pKa = 5.78 HH111 pKa = 6.51 TKK113 pKa = 10.64 SKK115 pKa = 10.07 TKK117 pKa = 10.16 INEE120 pKa = 3.74 DD121 pKa = 3.33 FKK123 pKa = 11.19 LYY125 pKa = 10.62 EE126 pKa = 4.41 YY127 pKa = 9.21 GTLPADD133 pKa = 3.97 KK134 pKa = 9.95 PNISAQVTKK143 pKa = 10.7 RR144 pKa = 11.84 SPEE147 pKa = 3.81 EE148 pKa = 3.85 KK149 pKa = 9.84 KK150 pKa = 10.67 RR151 pKa = 11.84 KK152 pKa = 9.51 LDD154 pKa = 3.81 DD155 pKa = 4.03 VIKK158 pKa = 10.27 EE159 pKa = 3.84 MRR161 pKa = 11.84 GMIEE165 pKa = 3.71 EE166 pKa = 4.28 GRR168 pKa = 11.84 EE169 pKa = 3.65 EE170 pKa = 3.78 EE171 pKa = 4.77 AFTKK175 pKa = 10.43 FPRR178 pKa = 11.84 NYY180 pKa = 8.62 LTYY183 pKa = 10.53 GEE185 pKa = 4.83 KK186 pKa = 10.15 LKK188 pKa = 11.43 AMISQRR194 pKa = 11.84 RR195 pKa = 11.84 DD196 pKa = 3.21 FFKK199 pKa = 11.26 SNGDD203 pKa = 3.21 PHH205 pKa = 7.75 IWLTGTPGSGKK216 pKa = 10.08 SAILQVIYY224 pKa = 9.42 PAYY227 pKa = 10.09 YY228 pKa = 10.59 NKK230 pKa = 10.47 NLDD233 pKa = 3.24 NRR235 pKa = 11.84 FFDD238 pKa = 4.51 LYY240 pKa = 9.83 QPKK243 pKa = 9.44 VHH245 pKa = 5.45 THH247 pKa = 5.74 MLLQDD252 pKa = 3.48 VDD254 pKa = 3.69 HH255 pKa = 6.33 TTVEE259 pKa = 4.07 KK260 pKa = 11.04 LGVQFLKK267 pKa = 9.97 TICDD271 pKa = 3.43 EE272 pKa = 4.43 AGFPVDD278 pKa = 4.12 QKK280 pKa = 11.81 YY281 pKa = 7.52 KK282 pKa = 9.13 TPQLARR288 pKa = 11.84 TTVLVSSNFTINDD301 pKa = 4.09 VIPEE305 pKa = 4.14 DD306 pKa = 3.5 MPGRR310 pKa = 11.84 NEE312 pKa = 3.8 NLTALRR318 pKa = 11.84 RR319 pKa = 11.84 RR320 pKa = 11.84 FYY322 pKa = 10.4 EE323 pKa = 3.86 VNVRR327 pKa = 11.84 VLLQILGLKK336 pKa = 9.97 LLSKK340 pKa = 11.34 YY341 pKa = 9.4 EE342 pKa = 4.04 LNGLKK347 pKa = 10.14 RR348 pKa = 11.84 VGNQDD353 pKa = 3.07 PTKK356 pKa = 11.06 LFMSWDD362 pKa = 3.7 YY363 pKa = 11.28 TRR365 pKa = 11.84 DD366 pKa = 3.31 IPTGEE371 pKa = 4.07 SLKK374 pKa = 9.76 TAKK377 pKa = 10.06 EE378 pKa = 3.81 YY379 pKa = 10.29 QQIIKK384 pKa = 10.16 DD385 pKa = 3.71 HH386 pKa = 6.4 YY387 pKa = 9.56 YY388 pKa = 9.14 GTNTSPHH395 pKa = 6.31 SSQQ398 pKa = 3.59
Molecular weight: 46.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.245
IPC2_protein 8.287
IPC_protein 8.2
Toseland 8.785
ProMoST 8.668
Dawson 9.121
Bjellqvist 8.902
Wikipedia 9.297
Rodwell 9.282
Grimsley 9.18
Solomon 9.194
Lehninger 9.151
Nozaki 8.916
DTASelect 8.858
Thurlkill 8.96
EMBOSS 9.238
Sillero 9.107
Patrickios 4.507
IPC_peptide 9.18
IPC2_peptide 7.512
IPC2.peptide.svr19 7.596
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
996
152
446
332.0
37.79
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.124 ± 1.951
0.602 ± 0.05
7.229 ± 0.428
6.526 ± 1.919
3.514 ± 0.233
6.627 ± 1.571
2.51 ± 0.768
4.518 ± 0.625
5.823 ± 1.58
9.237 ± 0.816
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.108 ± 1.051
7.329 ± 1.095
4.92 ± 0.947
3.916 ± 0.558
5.321 ± 0.695
4.819 ± 1.03
6.124 ± 0.691
6.325 ± 0.755
1.104 ± 0.177
5.321 ± 1.142
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here