Alloprevotella rava F0323

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Prevotellaceae; Alloprevotella; Alloprevotella rava

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2009 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G5GBY3|G5GBY3_9BACT RNA polymerase sigma-54 factor OS=Alloprevotella rava F0323 OX=679199 GN=HMPREF9332_01084 PE=3 SV=1
MM1 pKa = 7.47KK2 pKa = 10.2KK3 pKa = 10.24YY4 pKa = 10.61RR5 pKa = 11.84CTVCDD10 pKa = 3.17WVYY13 pKa = 11.11DD14 pKa = 4.08PEE16 pKa = 5.57VGDD19 pKa = 3.97PDD21 pKa = 4.69AGIEE25 pKa = 4.03PGTAFEE31 pKa = 5.45DD32 pKa = 3.89LPEE35 pKa = 5.52DD36 pKa = 4.66FVCPLCGVGKK46 pKa = 10.28DD47 pKa = 3.4DD48 pKa = 4.55FEE50 pKa = 4.73VVEE53 pKa = 4.39EE54 pKa = 4.2

Molecular weight: 6.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G5GC42|G5GC42_9BACT Uncharacterized protein OS=Alloprevotella rava F0323 OX=679199 GN=HMPREF9332_01143 PE=4 SV=1
MM1 pKa = 7.5LRR3 pKa = 11.84QFLIRR8 pKa = 11.84LLIAPIRR15 pKa = 11.84FYY17 pKa = 11.28QRR19 pKa = 11.84FISPLTPPSCRR30 pKa = 11.84FTPTCSQYY38 pKa = 11.41AVEE41 pKa = 4.97ALRR44 pKa = 11.84KK45 pKa = 9.3HH46 pKa = 6.03GPIKK50 pKa = 10.75GLLLSIWRR58 pKa = 11.84ILRR61 pKa = 11.84CNPWGGSGYY70 pKa = 10.99DD71 pKa = 3.53PVPP74 pKa = 3.61

Molecular weight: 8.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2009

0

2009

732668

43

2639

364.7

41.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.731 ± 0.053

1.225 ± 0.021

5.33 ± 0.036

6.262 ± 0.054

4.61 ± 0.037

6.687 ± 0.049

2.064 ± 0.024

6.343 ± 0.049

6.203 ± 0.049

9.456 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.581 ± 0.027

5.008 ± 0.046

3.785 ± 0.033

3.859 ± 0.03

5.105 ± 0.047

6.156 ± 0.048

5.972 ± 0.055

6.399 ± 0.045

1.159 ± 0.021

4.064 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski