Lactobacillus phage iLp1308
Average proteome isoelectric point is 6.51
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0P0HRT3|A0A0P0HRT3_9CAUD Erf family protein OS=Lactobacillus phage iLp1308 OX=1739611 GN=iLP1308_31 PE=4 SV=1
MM1 pKa = 7.43 TNYY4 pKa = 9.64 DD5 pKa = 3.42 QCALLYY11 pKa = 10.57 SWGIDD16 pKa = 3.18 LTPYY20 pKa = 9.0 VPVMITPDD28 pKa = 3.2 QYY30 pKa = 11.8 KK31 pKa = 10.28 QITGSDD37 pKa = 3.78 YY38 pKa = 11.17 VASKK42 pKa = 10.87 SS43 pKa = 3.49
Molecular weight: 4.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.045
IPC2_protein 4.215
IPC_protein 3.91
Toseland 3.706
ProMoST 4.177
Dawson 3.961
Bjellqvist 4.139
Wikipedia 4.024
Rodwell 3.757
Grimsley 3.643
Solomon 3.91
Lehninger 3.872
Nozaki 4.139
DTASelect 4.418
Thurlkill 3.846
EMBOSS 4.024
Sillero 4.05
Patrickios 0.006
IPC_peptide 3.897
IPC2_peptide 4.012
IPC2.peptide.svr19 3.955
Protein with the highest isoelectric point:
>tr|A0A0P0I3H2|A0A0P0I3H2_9CAUD Uncharacterized protein OS=Lactobacillus phage iLp1308 OX=1739611 GN=iLP1308_22 PE=4 SV=1
MM1 pKa = 7.31 SKK3 pKa = 10.62 VGIGYY8 pKa = 9.45 RR9 pKa = 11.84 IQLKK13 pKa = 10.61 GMDD16 pKa = 4.07 KK17 pKa = 10.84 LVAGLLKK24 pKa = 9.97 RR25 pKa = 11.84 AKK27 pKa = 9.4 MDD29 pKa = 3.34 VVKK32 pKa = 10.79 QIVKK36 pKa = 9.63 QQTAQLQTRR45 pKa = 11.84 AQQMAGTVYY54 pKa = 10.78 AHH56 pKa = 6.42 PTGATKK62 pKa = 10.42 RR63 pKa = 11.84 GIKK66 pKa = 10.04 LAITDD71 pKa = 3.83 SGLTGIVAMTQEE83 pKa = 4.3 YY84 pKa = 10.78 NPYY87 pKa = 8.69 TEE89 pKa = 3.9 NGTRR93 pKa = 11.84 FMRR96 pKa = 11.84 ARR98 pKa = 11.84 PVLKK102 pKa = 10.0 PAFLYY107 pKa = 10.49 QKK109 pKa = 9.96 IQFINQLKK117 pKa = 9.42 QAAKK121 pKa = 10.35
Molecular weight: 13.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.259
IPC2_protein 10.028
IPC_protein 10.409
Toseland 10.789
ProMoST 10.394
Dawson 10.891
Bjellqvist 10.511
Wikipedia 11.023
Rodwell 11.433
Grimsley 10.921
Solomon 10.935
Lehninger 10.921
Nozaki 10.745
DTASelect 10.511
Thurlkill 10.774
EMBOSS 11.169
Sillero 10.804
Patrickios 11.155
IPC_peptide 10.935
IPC2_peptide 9.107
IPC2.peptide.svr19 8.485
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
50
0
50
10640
23
1029
212.8
23.67
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.919 ± 0.543
0.461 ± 0.125
6.842 ± 0.512
5.066 ± 0.403
3.731 ± 0.333
6.983 ± 0.452
1.429 ± 0.206
6.024 ± 0.299
6.88 ± 0.447
7.425 ± 0.302
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.585 ± 0.196
5.31 ± 0.235
3.205 ± 0.222
4.925 ± 0.262
4.032 ± 0.387
6.917 ± 0.442
7.096 ± 0.417
6.541 ± 0.266
1.673 ± 0.238
3.957 ± 0.358
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here