Amino acid dipepetide frequency for Lactobacillus phage iLp1308

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.271AlaAla: 8.271 ± 1.249
0.564AlaCys: 0.564 ± 0.238
7.426AlaAsp: 7.426 ± 0.973
4.7AlaGlu: 4.7 ± 0.72
3.572AlaPhe: 3.572 ± 0.63
6.016AlaGly: 6.016 ± 1.127
1.222AlaHis: 1.222 ± 0.366
4.888AlaIle: 4.888 ± 1.01
5.922AlaLys: 5.922 ± 0.981
6.11AlaLeu: 6.11 ± 0.658
2.914AlaMet: 2.914 ± 0.611
5.546AlaAsn: 5.546 ± 0.87
1.598AlaPro: 1.598 ± 0.444
3.572AlaGln: 3.572 ± 0.504
4.042AlaArg: 4.042 ± 0.756
5.734AlaSer: 5.734 ± 0.752
6.486AlaThr: 6.486 ± 0.788
6.016AlaVal: 6.016 ± 1.109
1.222AlaTrp: 1.222 ± 0.569
3.384AlaTyr: 3.384 ± 0.685
0.0AlaXaa: 0.0 ± 0.0
Cys
0.282CysAla: 0.282 ± 0.154
0.094CysCys: 0.094 ± 0.088
0.47CysAsp: 0.47 ± 0.181
0.376CysGlu: 0.376 ± 0.173
0.094CysPhe: 0.094 ± 0.093
0.47CysGly: 0.47 ± 0.284
0.094CysHis: 0.094 ± 0.092
0.188CysIle: 0.188 ± 0.12
0.47CysLys: 0.47 ± 0.23
0.376CysLeu: 0.376 ± 0.175
0.0CysMet: 0.0 ± 0.0
0.376CysAsn: 0.376 ± 0.193
0.188CysPro: 0.188 ± 0.125
0.188CysGln: 0.188 ± 0.135
0.282CysArg: 0.282 ± 0.164
0.094CysSer: 0.094 ± 0.091
0.094CysThr: 0.094 ± 0.083
0.282CysVal: 0.282 ± 0.151
0.188CysTrp: 0.188 ± 0.123
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
5.076AspAla: 5.076 ± 0.757
0.0AspCys: 0.0 ± 0.0
5.358AspAsp: 5.358 ± 0.997
4.794AspGlu: 4.794 ± 0.928
2.538AspPhe: 2.538 ± 0.414
7.332AspGly: 7.332 ± 1.141
1.504AspHis: 1.504 ± 0.35
3.666AspIle: 3.666 ± 0.542
4.982AspLys: 4.982 ± 1.26
4.888AspLeu: 4.888 ± 0.547
1.692AspMet: 1.692 ± 0.349
3.102AspAsn: 3.102 ± 0.656
2.538AspPro: 2.538 ± 0.505
2.726AspGln: 2.726 ± 0.664
3.008AspArg: 3.008 ± 0.509
4.136AspSer: 4.136 ± 0.643
3.478AspThr: 3.478 ± 0.667
3.76AspVal: 3.76 ± 0.542
1.222AspTrp: 1.222 ± 0.277
2.632AspTyr: 2.632 ± 0.531
0.0AspXaa: 0.0 ± 0.0
Glu
4.042GluAla: 4.042 ± 0.784
0.282GluCys: 0.282 ± 0.159
2.726GluAsp: 2.726 ± 0.645
2.068GluGlu: 2.068 ± 0.43
1.692GluPhe: 1.692 ± 0.433
3.29GluGly: 3.29 ± 0.511
1.034GluHis: 1.034 ± 0.323
3.008GluIle: 3.008 ± 0.53
3.384GluLys: 3.384 ± 0.633
4.982GluLeu: 4.982 ± 0.693
1.786GluMet: 1.786 ± 0.491
2.632GluAsn: 2.632 ± 0.417
1.316GluPro: 1.316 ± 0.409
2.632GluGln: 2.632 ± 0.529
2.256GluArg: 2.256 ± 0.415
3.008GluSer: 3.008 ± 0.542
3.76GluThr: 3.76 ± 0.637
4.042GluVal: 4.042 ± 0.609
0.752GluTrp: 0.752 ± 0.223
1.974GluTyr: 1.974 ± 0.493
0.0GluXaa: 0.0 ± 0.0
Phe
3.102PheAla: 3.102 ± 0.528
0.188PheCys: 0.188 ± 0.185
2.632PheAsp: 2.632 ± 0.395
1.786PheGlu: 1.786 ± 0.397
1.786PhePhe: 1.786 ± 0.698
2.444PheGly: 2.444 ± 0.538
0.188PheHis: 0.188 ± 0.131
1.786PheIle: 1.786 ± 0.328
2.632PheLys: 2.632 ± 0.453
2.538PheLeu: 2.538 ± 0.646
1.128PheMet: 1.128 ± 0.294
2.35PheAsn: 2.35 ± 0.401
1.034PhePro: 1.034 ± 0.396
1.222PheGln: 1.222 ± 0.343
1.128PheArg: 1.128 ± 0.373
3.666PheSer: 3.666 ± 1.028
3.29PheThr: 3.29 ± 0.566
2.82PheVal: 2.82 ± 0.451
0.47PheTrp: 0.47 ± 0.233
1.128PheTyr: 1.128 ± 0.278
0.0PheXaa: 0.0 ± 0.0
Gly
5.734GlyAla: 5.734 ± 0.885
0.094GlyCys: 0.094 ± 0.078
4.606GlyAsp: 4.606 ± 1.333
3.102GlyGlu: 3.102 ± 0.549
2.35GlyPhe: 2.35 ± 0.524
4.136GlyGly: 4.136 ± 0.559
1.128GlyHis: 1.128 ± 0.434
6.486GlyIle: 6.486 ± 1.475
5.076GlyLys: 5.076 ± 0.584
5.264GlyLeu: 5.264 ± 1.231
2.538GlyMet: 2.538 ± 0.486
3.102GlyAsn: 3.102 ± 0.574
1.41GlyPro: 1.41 ± 0.303
2.538GlyGln: 2.538 ± 0.487
2.538GlyArg: 2.538 ± 0.428
5.828GlySer: 5.828 ± 0.778
5.922GlyThr: 5.922 ± 0.768
4.23GlyVal: 4.23 ± 0.689
0.94GlyTrp: 0.94 ± 0.36
2.82GlyTyr: 2.82 ± 0.566
0.0GlyXaa: 0.0 ± 0.0
His
0.564HisAla: 0.564 ± 0.207
0.282HisCys: 0.282 ± 0.178
1.316HisAsp: 1.316 ± 0.31
0.564HisGlu: 0.564 ± 0.238
0.47HisPhe: 0.47 ± 0.167
1.034HisGly: 1.034 ± 0.279
0.282HisHis: 0.282 ± 0.145
0.752HisIle: 0.752 ± 0.271
0.94HisLys: 0.94 ± 0.339
1.222HisLeu: 1.222 ± 0.315
0.376HisMet: 0.376 ± 0.178
0.188HisAsn: 0.188 ± 0.112
0.376HisPro: 0.376 ± 0.189
0.94HisGln: 0.94 ± 0.259
0.188HisArg: 0.188 ± 0.126
0.94HisSer: 0.94 ± 0.317
1.222HisThr: 1.222 ± 0.358
1.128HisVal: 1.128 ± 0.338
0.188HisTrp: 0.188 ± 0.111
1.316HisTyr: 1.316 ± 0.408
0.0HisXaa: 0.0 ± 0.0
Ile
5.734IleAla: 5.734 ± 0.701
0.376IleCys: 0.376 ± 0.167
4.324IleAsp: 4.324 ± 0.629
3.384IleGlu: 3.384 ± 0.647
1.41IlePhe: 1.41 ± 0.367
4.23IleGly: 4.23 ± 0.676
0.658IleHis: 0.658 ± 0.286
3.008IleIle: 3.008 ± 0.581
4.042IleLys: 4.042 ± 0.643
2.82IleLeu: 2.82 ± 0.461
1.034IleMet: 1.034 ± 0.309
4.324IleAsn: 4.324 ± 0.677
2.35IlePro: 2.35 ± 0.386
2.726IleGln: 2.726 ± 0.511
1.786IleArg: 1.786 ± 0.418
4.606IleSer: 4.606 ± 0.667
5.17IleThr: 5.17 ± 0.56
4.512IleVal: 4.512 ± 0.792
1.786IleTrp: 1.786 ± 0.816
1.974IleTyr: 1.974 ± 0.551
0.0IleXaa: 0.0 ± 0.0
Lys
7.144LysAla: 7.144 ± 1.196
0.376LysCys: 0.376 ± 0.195
3.572LysAsp: 3.572 ± 0.585
3.102LysGlu: 3.102 ± 0.49
2.162LysPhe: 2.162 ± 0.486
5.358LysGly: 5.358 ± 1.331
0.658LysHis: 0.658 ± 0.264
3.478LysIle: 3.478 ± 0.426
3.854LysLys: 3.854 ± 0.776
5.734LysLeu: 5.734 ± 0.965
2.162LysMet: 2.162 ± 0.541
3.102LysAsn: 3.102 ± 0.53
3.196LysPro: 3.196 ± 0.6
5.076LysGln: 5.076 ± 0.896
3.76LysArg: 3.76 ± 0.657
3.76LysSer: 3.76 ± 0.535
4.982LysThr: 4.982 ± 0.639
3.666LysVal: 3.666 ± 0.468
0.658LysTrp: 0.658 ± 0.24
2.914LysTyr: 2.914 ± 0.451
0.0LysXaa: 0.0 ± 0.0
Leu
6.392LeuAla: 6.392 ± 0.79
0.188LeuCys: 0.188 ± 0.121
4.794LeuAsp: 4.794 ± 0.616
4.042LeuGlu: 4.042 ± 0.576
1.974LeuPhe: 1.974 ± 0.418
4.324LeuGly: 4.324 ± 0.776
0.846LeuHis: 0.846 ± 0.262
4.982LeuIle: 4.982 ± 0.719
5.828LeuLys: 5.828 ± 1.051
4.794LeuLeu: 4.794 ± 0.538
1.598LeuMet: 1.598 ± 0.362
3.76LeuAsn: 3.76 ± 0.635
2.632LeuPro: 2.632 ± 0.517
4.042LeuGln: 4.042 ± 0.449
3.384LeuArg: 3.384 ± 0.597
5.734LeuSer: 5.734 ± 0.802
5.64LeuThr: 5.64 ± 0.715
4.606LeuVal: 4.606 ± 0.687
1.598LeuTrp: 1.598 ± 0.577
3.102LeuTyr: 3.102 ± 0.568
0.0LeuXaa: 0.0 ± 0.0
Met
2.444MetAla: 2.444 ± 0.566
0.0MetCys: 0.0 ± 0.0
1.222MetAsp: 1.222 ± 0.346
1.128MetGlu: 1.128 ± 0.255
0.564MetPhe: 0.564 ± 0.192
1.786MetGly: 1.786 ± 0.373
0.282MetHis: 0.282 ± 0.139
1.692MetIle: 1.692 ± 0.464
1.504MetLys: 1.504 ± 0.391
2.162MetLeu: 2.162 ± 0.494
0.658MetMet: 0.658 ± 0.247
2.256MetAsn: 2.256 ± 0.452
1.034MetPro: 1.034 ± 0.253
1.41MetGln: 1.41 ± 0.358
1.034MetArg: 1.034 ± 0.326
1.598MetSer: 1.598 ± 0.354
2.914MetThr: 2.914 ± 0.314
1.128MetVal: 1.128 ± 0.385
0.188MetTrp: 0.188 ± 0.216
0.846MetTyr: 0.846 ± 0.262
0.0MetXaa: 0.0 ± 0.0
Asn
4.888AsnAla: 4.888 ± 0.567
0.282AsnCys: 0.282 ± 0.15
4.7AsnAsp: 4.7 ± 0.8
2.256AsnGlu: 2.256 ± 0.536
2.162AsnPhe: 2.162 ± 0.439
5.64AsnGly: 5.64 ± 0.779
0.846AsnHis: 0.846 ± 0.245
2.82AsnIle: 2.82 ± 0.503
3.008AsnLys: 3.008 ± 0.62
3.29AsnLeu: 3.29 ± 0.49
1.128AsnMet: 1.128 ± 0.313
2.35AsnAsn: 2.35 ± 0.345
2.444AsnPro: 2.444 ± 0.481
2.726AsnGln: 2.726 ± 0.559
2.632AsnArg: 2.632 ± 0.535
3.384AsnSer: 3.384 ± 0.687
2.82AsnThr: 2.82 ± 0.597
2.726AsnVal: 2.726 ± 0.442
0.94AsnTrp: 0.94 ± 0.265
2.068AsnTyr: 2.068 ± 0.521
0.0AsnXaa: 0.0 ± 0.0
Pro
2.914ProAla: 2.914 ± 0.463
0.0ProCys: 0.0 ± 0.0
1.974ProAsp: 1.974 ± 0.459
2.726ProGlu: 2.726 ± 0.608
1.128ProPhe: 1.128 ± 0.326
1.786ProGly: 1.786 ± 0.379
0.376ProHis: 0.376 ± 0.181
2.726ProIle: 2.726 ± 0.416
3.666ProLys: 3.666 ± 0.622
1.786ProLeu: 1.786 ± 0.365
0.564ProMet: 0.564 ± 0.196
1.128ProAsn: 1.128 ± 0.324
0.188ProPro: 0.188 ± 0.133
1.034ProGln: 1.034 ± 0.292
0.564ProArg: 0.564 ± 0.203
3.102ProSer: 3.102 ± 0.58
1.88ProThr: 1.88 ± 0.461
2.444ProVal: 2.444 ± 0.533
0.846ProTrp: 0.846 ± 0.216
1.222ProTyr: 1.222 ± 0.335
0.0ProXaa: 0.0 ± 0.0
Gln
5.452GlnAla: 5.452 ± 0.683
0.376GlnCys: 0.376 ± 0.183
2.35GlnAsp: 2.35 ± 0.654
2.538GlnGlu: 2.538 ± 0.485
2.35GlnPhe: 2.35 ± 0.419
2.726GlnGly: 2.726 ± 0.383
0.846GlnHis: 0.846 ± 0.343
2.35GlnIle: 2.35 ± 0.445
2.726GlnLys: 2.726 ± 0.534
3.948GlnLeu: 3.948 ± 0.644
1.316GlnMet: 1.316 ± 0.296
2.068GlnAsn: 2.068 ± 0.447
1.41GlnPro: 1.41 ± 0.322
3.102GlnGln: 3.102 ± 0.544
1.974GlnArg: 1.974 ± 0.391
2.82GlnSer: 2.82 ± 0.444
3.478GlnThr: 3.478 ± 0.709
3.948GlnVal: 3.948 ± 0.608
1.128GlnTrp: 1.128 ± 0.233
2.35GlnTyr: 2.35 ± 0.445
0.0GlnXaa: 0.0 ± 0.0
Arg
3.008ArgAla: 3.008 ± 0.546
0.376ArgCys: 0.376 ± 0.186
1.786ArgAsp: 1.786 ± 0.383
2.82ArgGlu: 2.82 ± 0.52
1.974ArgPhe: 1.974 ± 0.522
1.88ArgGly: 1.88 ± 0.397
0.47ArgHis: 0.47 ± 0.236
2.538ArgIle: 2.538 ± 0.655
3.666ArgLys: 3.666 ± 0.558
4.042ArgLeu: 4.042 ± 0.614
0.658ArgMet: 0.658 ± 0.276
2.444ArgAsn: 2.444 ± 0.384
1.128ArgPro: 1.128 ± 0.275
2.82ArgGln: 2.82 ± 0.599
2.162ArgArg: 2.162 ± 0.461
1.786ArgSer: 1.786 ± 0.355
2.068ArgThr: 2.068 ± 0.45
2.162ArgVal: 2.162 ± 0.446
0.376ArgTrp: 0.376 ± 0.156
2.162ArgTyr: 2.162 ± 0.596
0.0ArgXaa: 0.0 ± 0.0
Ser
5.64SerAla: 5.64 ± 1.092
0.0SerCys: 0.0 ± 0.0
5.546SerAsp: 5.546 ± 0.872
2.82SerGlu: 2.82 ± 0.657
3.384SerPhe: 3.384 ± 0.71
6.11SerGly: 6.11 ± 1.096
0.658SerHis: 0.658 ± 0.227
3.478SerIle: 3.478 ± 0.646
5.734SerLys: 5.734 ± 0.789
4.7SerLeu: 4.7 ± 0.633
1.504SerMet: 1.504 ± 0.359
4.136SerAsn: 4.136 ± 0.61
2.444SerPro: 2.444 ± 0.545
3.008SerGln: 3.008 ± 0.823
1.786SerArg: 1.786 ± 0.411
5.264SerSer: 5.264 ± 0.776
4.512SerThr: 4.512 ± 0.795
4.888SerVal: 4.888 ± 0.674
1.034SerTrp: 1.034 ± 0.294
2.538SerTyr: 2.538 ± 0.473
0.0SerXaa: 0.0 ± 0.0
Thr
7.144ThrAla: 7.144 ± 0.866
0.282ThrCys: 0.282 ± 0.151
5.076ThrAsp: 5.076 ± 0.584
2.726ThrGlu: 2.726 ± 0.412
3.102ThrPhe: 3.102 ± 0.546
3.76ThrGly: 3.76 ± 0.462
1.598ThrHis: 1.598 ± 0.533
4.888ThrIle: 4.888 ± 0.547
3.572ThrLys: 3.572 ± 0.421
5.452ThrLeu: 5.452 ± 0.748
1.504ThrMet: 1.504 ± 0.319
3.196ThrAsn: 3.196 ± 0.506
3.384ThrPro: 3.384 ± 0.68
3.008ThrGln: 3.008 ± 0.516
2.538ThrArg: 2.538 ± 0.5
4.606ThrSer: 4.606 ± 0.613
4.982ThrThr: 4.982 ± 0.6
6.486ThrVal: 6.486 ± 0.621
1.034ThrTrp: 1.034 ± 0.288
2.632ThrTyr: 2.632 ± 0.541
0.0ThrXaa: 0.0 ± 0.0
Val
7.144ValAla: 7.144 ± 0.939
0.282ValCys: 0.282 ± 0.145
4.606ValAsp: 4.606 ± 0.595
3.76ValGlu: 3.76 ± 0.827
1.692ValPhe: 1.692 ± 0.344
4.606ValGly: 4.606 ± 0.709
0.282ValHis: 0.282 ± 0.132
3.854ValIle: 3.854 ± 0.623
4.794ValLys: 4.794 ± 0.891
5.17ValLeu: 5.17 ± 0.725
2.068ValMet: 2.068 ± 0.426
3.478ValAsn: 3.478 ± 0.566
1.88ValPro: 1.88 ± 0.442
2.914ValGln: 2.914 ± 0.528
2.538ValArg: 2.538 ± 0.487
5.264ValSer: 5.264 ± 0.885
4.606ValThr: 4.606 ± 0.593
3.196ValVal: 3.196 ± 0.554
1.222ValTrp: 1.222 ± 0.484
2.068ValTyr: 2.068 ± 0.494
0.0ValXaa: 0.0 ± 0.0
Trp
0.846TrpAla: 0.846 ± 0.24
0.094TrpCys: 0.094 ± 0.096
0.846TrpAsp: 0.846 ± 0.278
0.47TrpGlu: 0.47 ± 0.179
0.564TrpPhe: 0.564 ± 0.231
1.128TrpGly: 1.128 ± 0.366
0.47TrpHis: 0.47 ± 0.184
1.222TrpIle: 1.222 ± 0.273
0.658TrpLys: 0.658 ± 0.242
1.786TrpLeu: 1.786 ± 0.427
0.282TrpMet: 0.282 ± 0.153
1.88TrpAsn: 1.88 ± 1.042
0.094TrpPro: 0.094 ± 0.106
1.316TrpGln: 1.316 ± 0.353
0.376TrpArg: 0.376 ± 0.177
1.598TrpSer: 1.598 ± 0.713
1.128TrpThr: 1.128 ± 0.343
1.128TrpVal: 1.128 ± 0.315
0.188TrpTrp: 0.188 ± 0.118
0.658TrpTyr: 0.658 ± 0.261
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.384TyrAla: 3.384 ± 0.415
0.47TyrCys: 0.47 ± 0.177
3.102TyrAsp: 3.102 ± 0.455
1.504TyrGlu: 1.504 ± 0.509
2.35TyrPhe: 2.35 ± 0.303
1.786TyrGly: 1.786 ± 0.384
0.846TyrHis: 0.846 ± 0.386
2.444TyrIle: 2.444 ± 0.521
2.35TyrLys: 2.35 ± 0.445
3.196TyrLeu: 3.196 ± 0.69
0.846TyrMet: 0.846 ± 0.276
1.692TyrAsn: 1.692 ± 0.386
1.41TyrPro: 1.41 ± 0.356
2.256TyrGln: 2.256 ± 0.448
2.35TyrArg: 2.35 ± 0.41
2.256TyrSer: 2.256 ± 0.517
2.538TyrThr: 2.538 ± 0.532
2.256TyrVal: 2.256 ± 0.511
0.752TyrTrp: 0.752 ± 0.293
1.786TyrTyr: 1.786 ± 0.464
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 50 proteins (10640 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski