Halomonas beimenensis
Average proteome isoelectric point is 6.2
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3771 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A291P556|A0A291P556_9GAMM Uncharacterized protein OS=Halomonas beimenensis OX=475662 GN=BEI_1030 PE=4 SV=1
MM1 pKa = 7.18 KK2 pKa = 10.01 TMHH5 pKa = 7.64 LITATAFAAAGLVAAQSAYY24 pKa = 10.4 AYY26 pKa = 9.67 QAGDD30 pKa = 3.01 IYY32 pKa = 11.49 VRR34 pKa = 11.84 GGIAKK39 pKa = 8.98 TEE41 pKa = 4.04 VTDD44 pKa = 5.13 DD45 pKa = 3.56 NGTLDD50 pKa = 3.42 VAGEE54 pKa = 4.15 LDD56 pKa = 3.82 ISDD59 pKa = 4.34 EE60 pKa = 4.21 SHH62 pKa = 6.56 LAYY65 pKa = 10.51 ALGYY69 pKa = 10.12 LFHH72 pKa = 7.67 DD73 pKa = 3.49 KK74 pKa = 10.4 WGMEE78 pKa = 3.97 LSGAEE83 pKa = 4.06 PVEE86 pKa = 4.3 HH87 pKa = 6.43 QLNTGNLGDD96 pKa = 3.41 IGGVDD101 pKa = 3.9 RR102 pKa = 11.84 MPVNLMVNYY111 pKa = 9.83 YY112 pKa = 9.74 PLGGTGAKK120 pKa = 8.37 VQPYY124 pKa = 9.84 VGAGLNYY131 pKa = 9.72 TRR133 pKa = 11.84 FSDD136 pKa = 4.31 EE137 pKa = 4.38 EE138 pKa = 4.58 LDD140 pKa = 4.0 GLDD143 pKa = 4.36 VDD145 pKa = 4.64 EE146 pKa = 4.82 SWGAVGQVGVDD157 pKa = 3.73 LAITDD162 pKa = 3.95 YY163 pKa = 11.57 LLAGAFARR171 pKa = 11.84 YY172 pKa = 9.93 ADD174 pKa = 3.39 VDD176 pKa = 3.78 ADD178 pKa = 3.86 VSVGGTDD185 pKa = 2.79 IGEE188 pKa = 4.41 AEE190 pKa = 4.52 VDD192 pKa = 3.44 PMTVGGVLTFRR203 pKa = 11.84 FF204 pKa = 3.96
Molecular weight: 21.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.727
IPC2_protein 3.91
IPC_protein 3.91
Toseland 3.694
ProMoST 4.075
Dawson 3.897
Bjellqvist 4.05
Wikipedia 3.834
Rodwell 3.732
Grimsley 3.605
Solomon 3.897
Lehninger 3.846
Nozaki 4.012
DTASelect 4.266
Thurlkill 3.745
EMBOSS 3.846
Sillero 4.024
Patrickios 1.138
IPC_peptide 3.884
IPC2_peptide 4.012
IPC2.peptide.svr19 3.907
Protein with the highest isoelectric point:
>tr|A0A291P291|A0A291P291_9GAMM Beta sliding clamp OS=Halomonas beimenensis OX=475662 GN=BEI_0002 PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.42 RR12 pKa = 11.84 KK13 pKa = 9.04 RR14 pKa = 11.84 AHH16 pKa = 6.12 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.3 NGRR28 pKa = 11.84 AVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.93 GRR39 pKa = 11.84 KK40 pKa = 8.85 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.257
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.705
Grimsley 13.042
Solomon 13.51
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.427
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.173
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3771
0
3771
1196699
30
1623
317.3
34.74
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.035 ± 0.055
0.947 ± 0.014
5.839 ± 0.034
6.79 ± 0.045
3.336 ± 0.026
8.675 ± 0.037
2.501 ± 0.019
4.161 ± 0.03
2.214 ± 0.03
11.781 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.348 ± 0.018
2.075 ± 0.024
5.326 ± 0.032
3.321 ± 0.025
8.225 ± 0.048
4.698 ± 0.025
4.763 ± 0.026
7.259 ± 0.031
1.501 ± 0.02
2.205 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here