Salinisphaera orenii MK-B5
Average proteome isoelectric point is 6.14
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3204 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A423PSG1|A0A423PSG1_9GAMM NAD(P) transhydrogenase subunit alpha OS=Salinisphaera orenii MK-B5 OX=856730 GN=SAOR_06020 PE=3 SV=1
MM1 pKa = 7.51 PQTLPHH7 pKa = 7.02 RR8 pKa = 11.84 LLTLLGPLALLALGGCVHH26 pKa = 6.92 SPPSDD31 pKa = 2.96 PWDD34 pKa = 4.44 PIEE37 pKa = 4.17 PVNRR41 pKa = 11.84 AVYY44 pKa = 9.96 QFNDD48 pKa = 3.26 KK49 pKa = 10.81 ADD51 pKa = 3.67 QYY53 pKa = 11.1 VLQPAARR60 pKa = 11.84 GYY62 pKa = 9.54 DD63 pKa = 3.24 TVTPDD68 pKa = 3.54 RR69 pKa = 11.84 VQTSVGNFFDD79 pKa = 3.96 NARR82 pKa = 11.84 MPITIVNSALQLKK95 pKa = 9.81 FDD97 pKa = 4.34 SFNVALGRR105 pKa = 11.84 FMINSTAGVAGLFDD119 pKa = 3.45 VASALEE125 pKa = 3.91 IDD127 pKa = 4.27 EE128 pKa = 4.87 PEE130 pKa = 4.03 EE131 pKa = 5.33 DD132 pKa = 3.49 FGQTFGYY139 pKa = 8.98 WGLGQGPYY147 pKa = 10.48 LVLPFLGPSTGRR159 pKa = 11.84 DD160 pKa = 3.54 FVGDD164 pKa = 3.8 LGDD167 pKa = 3.46 QFLYY171 pKa = 10.35 PIDD174 pKa = 4.16 YY175 pKa = 10.78 LDD177 pKa = 6.44 DD178 pKa = 4.87 IDD180 pKa = 6.53 DD181 pKa = 4.6 DD182 pKa = 3.96 YY183 pKa = 12.01 QYY185 pKa = 10.81 IIWGLRR191 pKa = 11.84 GLEE194 pKa = 3.93 LLNYY198 pKa = 9.1 RR199 pKa = 11.84 ASLLGFEE206 pKa = 5.1 DD207 pKa = 3.76 TLEE210 pKa = 4.13 QQFDD214 pKa = 3.74 PYY216 pKa = 11.51 AFLRR220 pKa = 11.84 GYY222 pKa = 10.04 YY223 pKa = 10.16 LEE225 pKa = 5.2 NRR227 pKa = 11.84 LKK229 pKa = 10.57 DD230 pKa = 3.73 VYY232 pKa = 11.21 DD233 pKa = 4.12 GDD235 pKa = 4.19 VPPEE239 pKa = 3.77 RR240 pKa = 11.84 MYY242 pKa = 11.58 GDD244 pKa = 4.13 DD245 pKa = 4.14 AADD248 pKa = 3.96 GEE250 pKa = 4.62
Molecular weight: 27.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.739
IPC2_protein 3.884
IPC_protein 3.897
Toseland 3.668
ProMoST 4.05
Dawson 3.897
Bjellqvist 4.075
Wikipedia 3.846
Rodwell 3.719
Grimsley 3.579
Solomon 3.897
Lehninger 3.846
Nozaki 4.012
DTASelect 4.291
Thurlkill 3.732
EMBOSS 3.859
Sillero 4.024
Patrickios 1.926
IPC_peptide 3.884
IPC2_peptide 3.999
IPC2.peptide.svr19 3.914
Protein with the highest isoelectric point:
>tr|A0A423PEL6|A0A423PEL6_9GAMM Histidine kinase OS=Salinisphaera orenii MK-B5 OX=856730 GN=SAOR_16370 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.44 RR3 pKa = 11.84 TYY5 pKa = 10.3 QPSNLHH11 pKa = 6.47 RR12 pKa = 11.84 KK13 pKa = 6.84 RR14 pKa = 11.84 THH16 pKa = 5.74 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 9.78 NGRR28 pKa = 11.84 RR29 pKa = 11.84 VLSARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.94 GRR39 pKa = 11.84 KK40 pKa = 8.83 RR41 pKa = 11.84 LAPP44 pKa = 3.99
Molecular weight: 5.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.431
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.34
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.076
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.079
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3204
0
3204
1027050
29
1594
320.6
34.8
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.468 ± 0.067
0.929 ± 0.014
6.69 ± 0.045
5.745 ± 0.042
3.403 ± 0.031
8.614 ± 0.036
2.319 ± 0.021
4.614 ± 0.025
2.074 ± 0.03
10.13 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.342 ± 0.02
2.367 ± 0.024
4.944 ± 0.026
2.971 ± 0.026
8.073 ± 0.052
4.888 ± 0.032
5.16 ± 0.03
7.44 ± 0.038
1.374 ± 0.018
2.455 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here