Salinisphaera orenii MK-B5

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Salinisphaerales; Salinisphaeraceae; Salinisphaera; Salinisphaera orenii

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3204 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A423PSG1|A0A423PSG1_9GAMM NAD(P) transhydrogenase subunit alpha OS=Salinisphaera orenii MK-B5 OX=856730 GN=SAOR_06020 PE=3 SV=1
MM1 pKa = 7.51PQTLPHH7 pKa = 7.02RR8 pKa = 11.84LLTLLGPLALLALGGCVHH26 pKa = 6.92SPPSDD31 pKa = 2.96PWDD34 pKa = 4.44PIEE37 pKa = 4.17PVNRR41 pKa = 11.84AVYY44 pKa = 9.96QFNDD48 pKa = 3.26KK49 pKa = 10.81ADD51 pKa = 3.67QYY53 pKa = 11.1VLQPAARR60 pKa = 11.84GYY62 pKa = 9.54DD63 pKa = 3.24TVTPDD68 pKa = 3.54RR69 pKa = 11.84VQTSVGNFFDD79 pKa = 3.96NARR82 pKa = 11.84MPITIVNSALQLKK95 pKa = 9.81FDD97 pKa = 4.34SFNVALGRR105 pKa = 11.84FMINSTAGVAGLFDD119 pKa = 3.45VASALEE125 pKa = 3.91IDD127 pKa = 4.27EE128 pKa = 4.87PEE130 pKa = 4.03EE131 pKa = 5.33DD132 pKa = 3.49FGQTFGYY139 pKa = 8.98WGLGQGPYY147 pKa = 10.48LVLPFLGPSTGRR159 pKa = 11.84DD160 pKa = 3.54FVGDD164 pKa = 3.8LGDD167 pKa = 3.46QFLYY171 pKa = 10.35PIDD174 pKa = 4.16YY175 pKa = 10.78LDD177 pKa = 6.44DD178 pKa = 4.87IDD180 pKa = 6.53DD181 pKa = 4.6DD182 pKa = 3.96YY183 pKa = 12.01QYY185 pKa = 10.81IIWGLRR191 pKa = 11.84GLEE194 pKa = 3.93LLNYY198 pKa = 9.1RR199 pKa = 11.84ASLLGFEE206 pKa = 5.1DD207 pKa = 3.76TLEE210 pKa = 4.13QQFDD214 pKa = 3.74PYY216 pKa = 11.51AFLRR220 pKa = 11.84GYY222 pKa = 10.04YY223 pKa = 10.16LEE225 pKa = 5.2NRR227 pKa = 11.84LKK229 pKa = 10.57DD230 pKa = 3.73VYY232 pKa = 11.21DD233 pKa = 4.12GDD235 pKa = 4.19VPPEE239 pKa = 3.77RR240 pKa = 11.84MYY242 pKa = 11.58GDD244 pKa = 4.13DD245 pKa = 4.14AADD248 pKa = 3.96GEE250 pKa = 4.62

Molecular weight: 27.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A423PEL6|A0A423PEL6_9GAMM Histidine kinase OS=Salinisphaera orenii MK-B5 OX=856730 GN=SAOR_16370 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.3QPSNLHH11 pKa = 6.47RR12 pKa = 11.84KK13 pKa = 6.84RR14 pKa = 11.84THH16 pKa = 5.74GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 9.78NGRR28 pKa = 11.84RR29 pKa = 11.84VLSARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.94GRR39 pKa = 11.84KK40 pKa = 8.83RR41 pKa = 11.84LAPP44 pKa = 3.99

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3204

0

3204

1027050

29

1594

320.6

34.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.468 ± 0.067

0.929 ± 0.014

6.69 ± 0.045

5.745 ± 0.042

3.403 ± 0.031

8.614 ± 0.036

2.319 ± 0.021

4.614 ± 0.025

2.074 ± 0.03

10.13 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.342 ± 0.02

2.367 ± 0.024

4.944 ± 0.026

2.971 ± 0.026

8.073 ± 0.052

4.888 ± 0.032

5.16 ± 0.03

7.44 ± 0.038

1.374 ± 0.018

2.455 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski