Amino acid dipepetide frequency for Salinisphaera orenii MK-B5

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	19.717AlaAla: 19.717 ± 0.229	1.313AlaCys: 1.313 ± 0.039	9.338AlaAsp: 9.338 ± 0.116	8.425AlaGlu: 8.425 ± 0.114	4.337AlaPhe: 4.337 ± 0.072	12.41AlaGly: 12.41 ± 0.131	2.974AlaHis: 2.974 ± 0.07	5.884AlaIle: 5.884 ± 0.08	2.245AlaLys: 2.245 ± 0.058	14.724AlaLeu: 14.724 ± 0.166	3.243AlaMet: 3.243 ± 0.06	2.687AlaAsn: 2.687 ± 0.051	6.052AlaPro: 6.052 ± 0.089	3.691AlaGln: 3.691 ± 0.066	11.304AlaArg: 11.304 ± 0.141	5.978AlaSer: 5.978 ± 0.074	6.323AlaThr: 6.323 ± 0.079	9.507AlaVal: 9.507 ± 0.106	1.787AlaTrp: 1.787 ± 0.046	2.74AlaTyr: 2.74 ± 0.057	0.0AlaXaa: 0.0 ± 0.0
Cys	1.174CysAla: 1.174 ± 0.038	0.137CysCys: 0.137 ± 0.012	0.603CysAsp: 0.603 ± 0.027	0.503CysGlu: 0.503 ± 0.02	0.314CysPhe: 0.314 ± 0.017	0.957CysGly: 0.957 ± 0.034	0.276CysHis: 0.276 ± 0.018	0.374CysIle: 0.374 ± 0.019	0.138CysLys: 0.138 ± 0.012	0.892CysLeu: 0.892 ± 0.029	0.186CysMet: 0.186 ± 0.013	0.238CysAsn: 0.238 ± 0.013	0.474CysPro: 0.474 ± 0.025	0.229CysGln: 0.229 ± 0.015	0.76CysArg: 0.76 ± 0.03	0.468CysSer: 0.468 ± 0.024	0.411CysThr: 0.411 ± 0.019	0.804CysVal: 0.804 ± 0.031	0.112CysTrp: 0.112 ± 0.01	0.238CysTyr: 0.238 ± 0.017	0.0CysXaa: 0.0 ± 0.0
Asp	9.241AspAla: 9.241 ± 0.108	0.601AspCys: 0.601 ± 0.025	5.121AspAsp: 5.121 ± 0.09	4.103AspGlu: 4.103 ± 0.068	2.239AspPhe: 2.239 ± 0.052	5.927AspGly: 5.927 ± 0.099	1.496AspHis: 1.496 ± 0.037	3.032AspIle: 3.032 ± 0.051	1.495AspLys: 1.495 ± 0.048	5.649AspLeu: 5.649 ± 0.082	1.523AspMet: 1.523 ± 0.037	1.711AspAsn: 1.711 ± 0.047	3.291AspPro: 3.291 ± 0.058	1.671AspGln: 1.671 ± 0.037	5.013AspArg: 5.013 ± 0.077	2.996AspSer: 2.996 ± 0.066	3.883AspThr: 3.883 ± 0.068	4.652AspVal: 4.652 ± 0.07	1.177AspTrp: 1.177 ± 0.034	2.081AspTyr: 2.081 ± 0.048	0.0AspXaa: 0.0 ± 0.0
Glu	8.075GluAla: 8.075 ± 0.112	0.388GluCys: 0.388 ± 0.018	2.902GluAsp: 2.902 ± 0.052	2.408GluGlu: 2.408 ± 0.061	1.597GluPhe: 1.597 ± 0.041	3.833GluGly: 3.833 ± 0.065	1.614GluHis: 1.614 ± 0.043	3.197GluIle: 3.197 ± 0.056	1.344GluLys: 1.344 ± 0.041	5.537GluLeu: 5.537 ± 0.077	1.301GluMet: 1.301 ± 0.045	1.46GluAsn: 1.46 ± 0.043	3.033GluPro: 3.033 ± 0.057	2.361GluGln: 2.361 ± 0.05	6.38GluArg: 6.38 ± 0.093	2.997GluSer: 2.997 ± 0.054	3.568GluThr: 3.568 ± 0.071	3.506GluVal: 3.506 ± 0.074	0.621GluTrp: 0.621 ± 0.024	1.323GluTyr: 1.323 ± 0.033	0.0GluXaa: 0.0 ± 0.0
Phe	4.379PheAla: 4.379 ± 0.07	0.353PheCys: 0.353 ± 0.021	2.905PheAsp: 2.905 ± 0.055	2.245PheGlu: 2.245 ± 0.049	1.345PhePhe: 1.345 ± 0.044	3.494PheGly: 3.494 ± 0.065	0.747PheHis: 0.747 ± 0.028	1.448PheIle: 1.448 ± 0.035	0.734PheLys: 0.734 ± 0.027	2.731PheLeu: 2.731 ± 0.06	0.802PheMet: 0.802 ± 0.031	0.96PheAsn: 0.96 ± 0.032	1.284PhePro: 1.284 ± 0.032	0.797PheGln: 0.797 ± 0.029	1.85PheArg: 1.85 ± 0.043	1.943PheSer: 1.943 ± 0.043	1.73PheThr: 1.73 ± 0.047	2.865PheVal: 2.865 ± 0.058	0.49PheTrp: 0.49 ± 0.021	0.931PheTyr: 0.931 ± 0.035	0.0PheXaa: 0.0 ± 0.0
Gly	9.877GlyAla: 9.877 ± 0.121	0.933GlyCys: 0.933 ± 0.031	5.554GlyAsp: 5.554 ± 0.084	5.22GlyGlu: 5.22 ± 0.066	3.256GlyPhe: 3.256 ± 0.063	7.36GlyGly: 7.36 ± 0.117	2.122GlyHis: 2.122 ± 0.05	3.99GlyIle: 3.99 ± 0.067	2.022GlyLys: 2.022 ± 0.054	9.299GlyLeu: 9.299 ± 0.118	2.523GlyMet: 2.523 ± 0.06	1.846GlyAsn: 1.846 ± 0.044	3.4GlyPro: 3.4 ± 0.065	2.601GlyGln: 2.601 ± 0.05	6.849GlyArg: 6.849 ± 0.09	4.153GlySer: 4.153 ± 0.068	4.298GlyThr: 4.298 ± 0.057	6.906GlyVal: 6.906 ± 0.084	1.435GlyTrp: 1.435 ± 0.042	2.494GlyTyr: 2.494 ± 0.052	0.0GlyXaa: 0.0 ± 0.0
His	2.98HisAla: 2.98 ± 0.065	0.286HisCys: 0.286 ± 0.017	1.564HisAsp: 1.564 ± 0.041	1.218HisGlu: 1.218 ± 0.035	0.854HisPhe: 0.854 ± 0.031	2.325HisGly: 2.325 ± 0.055	0.673HisHis: 0.673 ± 0.027	1.011HisIle: 1.011 ± 0.033	0.421HisLys: 0.421 ± 0.02	2.092HisLeu: 2.092 ± 0.045	0.508HisMet: 0.508 ± 0.021	0.504HisAsn: 0.504 ± 0.023	1.398HisPro: 1.398 ± 0.036	0.595HisGln: 0.595 ± 0.023	1.904HisArg: 1.904 ± 0.047	0.915HisSer: 0.915 ± 0.027	1.01HisThr: 1.01 ± 0.029	1.754HisVal: 1.754 ± 0.043	0.424HisTrp: 0.424 ± 0.019	0.756HisTyr: 0.756 ± 0.03	0.0HisXaa: 0.0 ± 0.0
Ile	6.824IleAla: 6.824 ± 0.093	0.442IleCys: 0.442 ± 0.019	4.086IleAsp: 4.086 ± 0.065	3.616IleGlu: 3.616 ± 0.066	1.275IlePhe: 1.275 ± 0.04	4.67IleGly: 4.67 ± 0.078	1.024IleHis: 1.024 ± 0.031	1.59IleIle: 1.59 ± 0.043	1.035IleLys: 1.035 ± 0.033	3.228IleLeu: 3.228 ± 0.065	0.874IleMet: 0.874 ± 0.032	1.351IleAsn: 1.351 ± 0.038	1.871IlePro: 1.871 ± 0.041	1.153IleGln: 1.153 ± 0.034	3.223IleArg: 3.223 ± 0.062	2.133IleSer: 2.133 ± 0.055	2.436IleThr: 2.436 ± 0.05	3.777IleVal: 3.777 ± 0.064	0.473IleTrp: 0.473 ± 0.02	1.056IleTyr: 1.056 ± 0.035	0.0IleXaa: 0.0 ± 0.0
Lys	2.619LysAla: 2.619 ± 0.065	0.119LysCys: 0.119 ± 0.01	1.019LysAsp: 1.019 ± 0.039	0.856LysGlu: 0.856 ± 0.04	0.503LysPhe: 0.503 ± 0.02	1.503LysGly: 1.503 ± 0.046	0.486LysHis: 0.486 ± 0.022	1.005LysIle: 1.005 ± 0.038	0.784LysLys: 0.784 ± 0.036	2.081LysLeu: 2.081 ± 0.048	0.445LysMet: 0.445 ± 0.02	0.57LysAsn: 0.57 ± 0.021	1.301LysPro: 1.301 ± 0.043	0.885LysGln: 0.885 ± 0.029	2.008LysArg: 2.008 ± 0.051	1.166LysSer: 1.166 ± 0.04	1.318LysThr: 1.318 ± 0.038	1.325LysVal: 1.325 ± 0.042	0.235LysTrp: 0.235 ± 0.017	0.512LysTyr: 0.512 ± 0.024	0.0LysXaa: 0.0 ± 0.0
Leu	15.371LeuAla: 15.371 ± 0.156	1.018LeuCys: 1.018 ± 0.033	7.0LeuAsp: 7.0 ± 0.095	5.56LeuGlu: 5.56 ± 0.083	3.619LeuPhe: 3.619 ± 0.069	8.477LeuGly: 8.477 ± 0.11	2.043LeuHis: 2.043 ± 0.046	4.788LeuIle: 4.788 ± 0.08	2.154LeuLys: 2.154 ± 0.045	9.744LeuLeu: 9.744 ± 0.14	2.284LeuMet: 2.284 ± 0.055	2.176LeuAsn: 2.176 ± 0.046	5.096LeuPro: 5.096 ± 0.08	2.617LeuGln: 2.617 ± 0.046	7.245LeuArg: 7.245 ± 0.095	5.664LeuSer: 5.664 ± 0.086	5.44LeuThr: 5.44 ± 0.079	7.527LeuVal: 7.527 ± 0.092	1.258LeuTrp: 1.258 ± 0.034	2.219LeuTyr: 2.219 ± 0.051	0.0LeuXaa: 0.0 ± 0.0
Met	3.231MetAla: 3.231 ± 0.058	0.184MetCys: 0.184 ± 0.014	1.262MetAsp: 1.262 ± 0.033	0.923MetGlu: 0.923 ± 0.033	0.674MetPhe: 0.674 ± 0.028	1.738MetGly: 1.738 ± 0.047	0.578MetHis: 0.578 ± 0.025	1.096MetIle: 1.096 ± 0.032	0.654MetLys: 0.654 ± 0.022	2.522MetLeu: 2.522 ± 0.049	0.488MetMet: 0.488 ± 0.022	0.74MetAsn: 0.74 ± 0.03	1.433MetPro: 1.433 ± 0.039	0.85MetGln: 0.85 ± 0.032	1.91MetArg: 1.91 ± 0.04	1.598MetSer: 1.598 ± 0.043	1.543MetThr: 1.543 ± 0.037	1.486MetVal: 1.486 ± 0.04	0.161MetTrp: 0.161 ± 0.011	0.349MetTyr: 0.349 ± 0.017	0.0MetXaa: 0.0 ± 0.0
Asn	3.187AsnAla: 3.187 ± 0.053	0.203AsnCys: 0.203 ± 0.015	1.593AsnAsp: 1.593 ± 0.05	1.226AsnGlu: 1.226 ± 0.038	0.784AsnPhe: 0.784 ± 0.03	2.087AsnGly: 2.087 ± 0.055	0.516AsnHis: 0.516 ± 0.025	1.122AsnIle: 1.122 ± 0.039	0.545AsnLys: 0.545 ± 0.023	2.224AsnLeu: 2.224 ± 0.051	0.511AsnMet: 0.511 ± 0.023	0.626AsnAsn: 0.626 ± 0.026	1.541AsnPro: 1.541 ± 0.037	0.708AsnGln: 0.708 ± 0.028	1.869AsnArg: 1.869 ± 0.042	0.925AsnSer: 0.925 ± 0.033	1.25AsnThr: 1.25 ± 0.039	1.801AsnVal: 1.801 ± 0.045	0.352AsnTrp: 0.352 ± 0.019	0.601AsnTyr: 0.601 ± 0.025	0.0AsnXaa: 0.0 ± 0.0
Pro	6.942ProAla: 6.942 ± 0.096	0.335ProCys: 0.335 ± 0.019	3.861ProAsp: 3.861 ± 0.068	3.612ProGlu: 3.612 ± 0.063	1.583ProPhe: 1.583 ± 0.037	4.64ProGly: 4.64 ± 0.072	1.021ProHis: 1.021 ± 0.03	2.03ProIle: 2.03 ± 0.039	1.003ProLys: 1.003 ± 0.033	4.577ProLeu: 4.577 ± 0.076	1.129ProMet: 1.129 ± 0.03	1.103ProAsn: 1.103 ± 0.038	2.372ProPro: 2.372 ± 0.059	1.408ProGln: 1.408 ± 0.041	3.33ProArg: 3.33 ± 0.064	2.351ProSer: 2.351 ± 0.052	2.351ProThr: 2.351 ± 0.052	3.932ProVal: 3.932 ± 0.062	0.698ProTrp: 0.698 ± 0.029	1.162ProTyr: 1.162 ± 0.036	0.0ProXaa: 0.0 ± 0.0
Gln	4.286GlnAla: 4.286 ± 0.068	0.277GlnCys: 0.277 ± 0.018	1.305GlnAsp: 1.305 ± 0.039	1.081GlnGlu: 1.081 ± 0.036	0.933GlnPhe: 0.933 ± 0.032	2.178GlnGly: 2.178 ± 0.046	0.633GlnHis: 0.633 ± 0.024	1.522GlnIle: 1.522 ± 0.039	0.647GlnLys: 0.647 ± 0.027	2.989GlnLeu: 2.989 ± 0.051	0.738GlnMet: 0.738 ± 0.028	0.743GlnAsn: 0.743 ± 0.029	1.733GlnPro: 1.733 ± 0.045	1.207GlnGln: 1.207 ± 0.043	3.024GlnArg: 3.024 ± 0.06	1.594GlnSer: 1.594 ± 0.041	1.606GlnThr: 1.606 ± 0.044	2.066GlnVal: 2.066 ± 0.045	0.448GlnTrp: 0.448 ± 0.021	0.704GlnTyr: 0.704 ± 0.024	0.0GlnXaa: 0.0 ± 0.0
Arg	10.0ArgAla: 10.0 ± 0.145	0.739ArgCys: 0.739 ± 0.03	5.078ArgAsp: 5.078 ± 0.075	5.026ArgGlu: 5.026 ± 0.085	2.996ArgPhe: 2.996 ± 0.068	5.646ArgGly: 5.646 ± 0.077	2.192ArgHis: 2.192 ± 0.053	4.082ArgIle: 4.082 ± 0.064	1.494ArgLys: 1.494 ± 0.042	9.43ArgLeu: 9.43 ± 0.12	2.036ArgMet: 2.036 ± 0.049	1.742ArgAsn: 1.742 ± 0.04	3.716ArgPro: 3.716 ± 0.068	2.583ArgGln: 2.583 ± 0.053	7.381ArgArg: 7.381 ± 0.117	3.496ArgSer: 3.496 ± 0.059	3.396ArgThr: 3.396 ± 0.064	6.042ArgVal: 6.042 ± 0.093	1.257ArgTrp: 1.257 ± 0.037	2.4ArgTyr: 2.4 ± 0.052	0.0ArgXaa: 0.0 ± 0.0
Ser	6.302SerAla: 6.302 ± 0.088	0.328SerCys: 0.328 ± 0.02	3.069SerAsp: 3.069 ± 0.06	2.773SerGlu: 2.773 ± 0.056	1.731SerPhe: 1.731 ± 0.04	4.978SerGly: 4.978 ± 0.068	1.088SerHis: 1.088 ± 0.027	2.193SerIle: 2.193 ± 0.04	1.049SerLys: 1.049 ± 0.038	4.937SerLeu: 4.937 ± 0.075	1.269SerMet: 1.269 ± 0.037	1.238SerAsn: 1.238 ± 0.038	2.418SerPro: 2.418 ± 0.05	1.523SerGln: 1.523 ± 0.039	3.779SerArg: 3.779 ± 0.053	2.521SerSer: 2.521 ± 0.055	2.358SerThr: 2.358 ± 0.052	3.595SerVal: 3.595 ± 0.06	0.635SerTrp: 0.635 ± 0.024	1.101SerTyr: 1.101 ± 0.034	0.0SerXaa: 0.0 ± 0.0
Thr	6.385ThrAla: 6.385 ± 0.078	0.44ThrCys: 0.44 ± 0.023	3.187ThrAsp: 3.187 ± 0.058	2.523ThrGlu: 2.523 ± 0.052	1.62ThrPhe: 1.62 ± 0.04	5.062ThrGly: 5.062 ± 0.08	1.197ThrHis: 1.197 ± 0.034	2.321ThrIle: 2.321 ± 0.053	0.77ThrLys: 0.77 ± 0.032	6.431ThrLeu: 6.431 ± 0.081	0.983ThrMet: 0.983 ± 0.036	1.006ThrAsn: 1.006 ± 0.032	3.459ThrPro: 3.459 ± 0.06	1.446ThrGln: 1.446 ± 0.034	4.127ThrArg: 4.127 ± 0.066	2.18ThrSer: 2.18 ± 0.049	2.712ThrThr: 2.712 ± 0.059	4.066ThrVal: 4.066 ± 0.055	0.599ThrTrp: 0.599 ± 0.025	1.087ThrTyr: 1.087 ± 0.036	0.0ThrXaa: 0.0 ± 0.0
Val	9.86ValAla: 9.86 ± 0.103	0.757ValCys: 0.757 ± 0.028	5.251ValAsp: 5.251 ± 0.073	4.372ValGlu: 4.372 ± 0.073	2.931ValPhe: 2.931 ± 0.063	5.745ValGly: 5.745 ± 0.08	1.557ValHis: 1.557 ± 0.04	3.789ValIle: 3.789 ± 0.067	1.376ValLys: 1.376 ± 0.043	7.685ValLeu: 7.685 ± 0.115	1.743ValMet: 1.743 ± 0.047	1.973ValAsn: 1.973 ± 0.05	3.577ValPro: 3.577 ± 0.06	1.88ValGln: 1.88 ± 0.046	5.009ValArg: 5.009 ± 0.07	3.896ValSer: 3.896 ± 0.061	4.159ValThr: 4.159 ± 0.066	6.135ValVal: 6.135 ± 0.094	0.935ValTrp: 0.935 ± 0.032	1.775ValTyr: 1.775 ± 0.041	0.0ValXaa: 0.0 ± 0.0
Trp	1.376TrpAla: 1.376 ± 0.036	0.153TrpCys: 0.153 ± 0.013	0.602TrpAsp: 0.602 ± 0.025	0.447TrpGlu: 0.447 ± 0.02	0.518TrpPhe: 0.518 ± 0.022	0.864TrpGly: 0.864 ± 0.031	0.415TrpHis: 0.415 ± 0.02	0.665TrpIle: 0.665 ± 0.028	0.271TrpLys: 0.271 ± 0.018	2.04TrpLeu: 2.04 ± 0.048	0.343TrpMet: 0.343 ± 0.019	0.364TrpAsn: 0.364 ± 0.019	0.786TrpPro: 0.786 ± 0.03	0.743TrpGln: 0.743 ± 0.027	1.421TrpArg: 1.421 ± 0.041	0.7TrpSer: 0.7 ± 0.03	0.607TrpThr: 0.607 ± 0.026	0.915TrpVal: 0.915 ± 0.03	0.199TrpTrp: 0.199 ± 0.015	0.31TrpTyr: 0.31 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.855TyrAla: 2.855 ± 0.055	0.282TyrCys: 0.282 ± 0.017	1.603TyrAsp: 1.603 ± 0.038	1.309TyrGlu: 1.309 ± 0.035	0.925TyrPhe: 0.925 ± 0.032	2.244TyrGly: 2.244 ± 0.052	0.541TyrHis: 0.541 ± 0.025	1.002TyrIle: 1.002 ± 0.036	0.56TyrLys: 0.56 ± 0.026	2.488TyrLeu: 2.488 ± 0.047	0.489TyrMet: 0.489 ± 0.026	0.632TyrAsn: 0.632 ± 0.026	1.203TyrPro: 1.203 ± 0.032	0.765TyrGln: 0.765 ± 0.028	2.345TyrArg: 2.345 ± 0.05	1.208TyrSer: 1.208 ± 0.034	1.203TyrThr: 1.203 ± 0.035	1.741TyrVal: 1.741 ± 0.044	0.441TyrTrp: 0.441 ± 0.022	0.711TyrTyr: 0.711 ± 0.029	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3204 proteins (1027050 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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